Details of the Target

General Information of Target

Target ID LDTP13671
Target Name Peptidyl-prolyl cis-trans isomerase E (PPIE)
Gene Name PPIE
Gene ID 10450
Synonyms
CYP33; Peptidyl-prolyl cis-trans isomerase E; PPIase E; EC 5.2.1.8; Cyclophilin E; Cyclophilin-33; Rotamase E
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MRTKAAGCAERRPLQPRTEAAAAPAGRAMPSEYTYVKLRSDCSRPSLQWYTRAQSKMRRP
SLLLKDILKCTLLVFGVWILYILKLNYTTEECDMKKMHYVDPDHVKRAQKYAQQVLQKEC
RPKFAKTSMALLFEHRYSVDLLPFVQKAPKDSEAESKYDPPFGFRKFSSKVQTLLELLPE
HDLPEHLKAKTCRRCVVIGSGGILHGLELGHTLNQFDVVIRLNSAPVEGYSEHVGNKTTI
RMTYPEGAPLSDLEYYSNDLFVAVLFKSVDFNWLQAMVKKETLPFWVRLFFWKQVAEKIP
LQPKHFRILNPVIIKETAFDILQYSEPQSRFWGRDKNVPTIGVIAVVLATHLCDEVSLAG
FGYDLNQPRTPLHYFDSQCMAAMNFQTMHNVTTETKFLLKLVKEGVVKDLSGGIDREF
Target Bioclass
Enzyme
Family
Cyclophilin-type PPIase family, PPIase E subfamily
Subcellular location
Nucleus
Function
Involved in pre-mRNA splicing as component of the spliceosome. Combines RNA-binding and PPIase activities. Binds mRNA and has a preference for single-stranded RNA molecules with poly-A and poly-U stretches, suggesting it binds to the poly(A)-region in the 3'-UTR of mRNA molecules. Catalyzes the cis-trans isomerization of proline imidic peptide bonds in proteins. Inhibits KMT2A activity; this requires proline isomerase activity.
Uniprot ID
Q9UNP9
Ensemble ID
ENST00000324379.10
HGNC ID
HGNC:9258

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
LNCaP clone FGC SNV: p.E130D .
MCC13 SNV: p.R78C .
MDST8 SNV: p.P28L .
MEWO SNV: p.P38L .
RKO SNV: p.M85T .
SW1783 SNV: p.R75C .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K147(3.29); K218(4.15); K83(8.47)  LDD0277  [1]
DBIA
 Probe Info  		C198(2.06)  LDD3427  [2]
BTD
 Probe Info  		C176(1.25)  LDD2096  [3]
Acrolein
 Probe Info  		H228(0.00); C252(0.00)  LDD0223  [4]
5E-2FA
 Probe Info  		N.A.  LDD2235  [5]
m-APA
 Probe Info  		N.A.  LDD2231  [5]
IA-alkyne
 Probe Info  		C198(0.00); C174(0.00); C176(0.00); C252(0.00)  LDD0036  [6]
Lodoacetamide azide
 Probe Info  		C252(0.00); C198(0.00)  LDD0037  [6]
NAIA_5
 Probe Info  		N.A.  LDD2223  [7]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-1
 Probe Info  		N.A.  LDD0068  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 A2058 C176(2.64)  LDD2253  [2]
 LDCM0023  KB03 COLO-679 C176(2.05)  LDD2722  [2]
 LDCM0024  KB05 SK-MEL-28 C198(2.06)  LDD3427  [2]
 LDCM0109  NEM HeLa H228(0.00); C252(0.00)  LDD0223  [4]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C176(1.25)  LDD2096  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Histone-lysine N-methyltransferase 2A (KMT2A) Histone-lysine methyltransferase family Q03164
RNA helicase aquarius (AQR) CWF11 family O60306
Fibroblast growth factor receptor 3 (FGFR3) Tyr protein kinase family P22607
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Pre-mRNA-splicing factor SYF1 (XAB2) Crooked-neck family Q9HCS7
Gelsolin (GSN) Villin/gelsolin family P06396

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
6 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
7 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
8 Proteome profiling reveals potential cellular targets of staurosporine using a clickable cell-permeable probe. Chem Commun (Camb). 2011 Oct 28;47(40):11306-8. doi: 10.1039/c1cc14824a. Epub 2011 Sep 16.