Details of the Target

General Information of Target

Target ID LDTP13420
Target Name N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase (NAGPA)
Gene Name NAGPA
Gene ID 51172
Synonyms
N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase; EC 3.1.4.45; Mannose 6-phosphate-uncovering enzyme; Phosphodiester alpha-GlcNAcase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDGDGDPESVGQPEEASPEEQPEEASAEEERPEDQQEEEAAAAAAYLDELPEPLLLRVLA
ALPAAELVQACRLVCLRWKELVDGAPLWLLKCQQEGLVPEGGVEEERDHWQQFYFLSKRR
RNLLRNPCGEEDLEGWCDVEHGGDGWRVEELPGDSGVEFTHDESVKKYFASSFEWCRKAQ
VIDLQAEGYWEELLDTTQPAIVVKDWYSGRSDAGCLYELTVKLLSEHENVLAEFSSGQVA
VPQDSDGGGWMEISHTFTDYGPGVRFVRFEHGGQDSVYWKGWFGARVTNSSVWVEP
Target Bioclass
Enzyme
Subcellular location
Golgi apparatus, Golgi stack membrane
Function
Catalyzes the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides by removing GlcNAc residues from GlcNAc-alpha-P-mannose moieties, which are formed in the first step. Also hydrolyzes UDP-GlcNAc, a sugar donor for Golgi N-acetylglucosaminyltransferases.
Uniprot ID
Q9UK23
Ensemble ID
ENST00000312251.8
HGNC ID
HGNC:17378
ChEMBL ID
CHEMBL5920

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.G74D .
HT SNV: p.S31A .
ICC8 SNV: p.W450C .
JURKAT SNV: p.A239D .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C408(1.06); C412(1.06)  LDD0531  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C408(1.06); C412(1.06)  LDD0531  [1]
 LDCM0279  AC2 HCT 116 C408(1.08); C412(1.08)  LDD0596  [1]
 LDCM0285  AC25 HCT 116 C408(1.09); C412(1.09)  LDD0602  [1]
 LDCM0286  AC26 HCT 116 C408(1.05); C412(1.05)  LDD0603  [1]
 LDCM0287  AC27 HCT 116 C408(1.15); C412(1.15)  LDD0604  [1]
 LDCM0288  AC28 HCT 116 C408(1.08); C412(1.08)  LDD0605  [1]
 LDCM0289  AC29 HCT 116 C408(1.20); C412(1.20)  LDD0606  [1]
 LDCM0290  AC3 HCT 116 C408(1.06); C412(1.06)  LDD0607  [1]
 LDCM0291  AC30 HCT 116 C408(1.12); C412(1.12)  LDD0608  [1]
 LDCM0292  AC31 HCT 116 C408(1.08); C412(1.08)  LDD0609  [1]
 LDCM0293  AC32 HCT 116 C408(1.31); C412(1.31)  LDD0610  [1]
 LDCM0294  AC33 HCT 116 C408(1.28); C412(1.28)  LDD0611  [1]
 LDCM0295  AC34 HCT 116 C408(1.15); C412(1.15)  LDD0612  [1]
 LDCM0301  AC4 HCT 116 C408(1.04); C412(1.04)  LDD0618  [1]
 LDCM0312  AC5 HCT 116 C408(1.04); C412(1.04)  LDD0629  [1]
 LDCM0332  AC68 HCT 116 C412(0.95); C408(1.08)  LDD0649  [1]
 LDCM0333  AC69 HCT 116 C412(1.12); C408(1.15)  LDD0650  [1]
 LDCM0335  AC70 HCT 116 C412(1.31); C408(1.91)  LDD0652  [1]
 LDCM0336  AC71 HCT 116 C412(0.86); C408(0.97)  LDD0653  [1]
 LDCM0337  AC72 HCT 116 C408(1.12); C412(1.14)  LDD0654  [1]
 LDCM0338  AC73 HCT 116 C412(1.14); C408(1.25)  LDD0655  [1]
 LDCM0339  AC74 HCT 116 C412(0.92); C408(1.11)  LDD0656  [1]
 LDCM0340  AC75 HCT 116 C412(1.20); C408(1.39)  LDD0657  [1]
 LDCM0341  AC76 HCT 116 C412(1.07); C408(1.28)  LDD0658  [1]
 LDCM0342  AC77 HCT 116 C412(1.25); C408(1.31)  LDD0659  [1]
 LDCM0343  AC78 HCT 116 C408(1.15); C412(1.23)  LDD0660  [1]
 LDCM0344  AC79 HCT 116 C408(1.11); C412(1.18)  LDD0661  [1]
 LDCM0346  AC80 HCT 116 C408(1.18); C412(1.22)  LDD0663  [1]
 LDCM0347  AC81 HCT 116 C412(1.10); C408(1.12)  LDD0664  [1]
 LDCM0348  AC82 HCT 116 C412(0.94); C408(1.13)  LDD0665  [1]
 LDCM0349  AC83 HCT 116 C408(1.40); C412(1.40)  LDD0666  [1]
 LDCM0350  AC84 HCT 116 C408(1.12); C412(1.12)  LDD0667  [1]
 LDCM0351  AC85 HCT 116 C408(1.06); C412(1.06)  LDD0668  [1]
 LDCM0352  AC86 HCT 116 C408(1.16); C412(1.16)  LDD0669  [1]
 LDCM0353  AC87 HCT 116 C408(1.17); C412(1.17)  LDD0670  [1]
 LDCM0354  AC88 HCT 116 C408(1.24); C412(1.24)  LDD0671  [1]
 LDCM0355  AC89 HCT 116 C408(1.54); C412(1.54)  LDD0672  [1]
 LDCM0357  AC90 HCT 116 C408(1.06); C412(1.06)  LDD0674  [1]
 LDCM0358  AC91 HCT 116 C408(1.36); C412(1.36)  LDD0675  [1]
 LDCM0359  AC92 HCT 116 C408(1.28); C412(1.28)  LDD0676  [1]
 LDCM0360  AC93 HCT 116 C408(1.06); C412(1.06)  LDD0677  [1]
 LDCM0361  AC94 HCT 116 C408(0.95); C412(0.95)  LDD0678  [1]
 LDCM0362  AC95 HCT 116 C408(1.16); C412(1.16)  LDD0679  [1]
 LDCM0363  AC96 HCT 116 C408(1.36); C412(1.36)  LDD0680  [1]
 LDCM0364  AC97 HCT 116 C408(1.13); C412(1.13)  LDD0681  [1]
 LDCM0367  CL1 HEK-293T C323(0.73); C408(0.94)  LDD1571  [2]
 LDCM0369  CL100 HCT 116 C408(0.97); C412(0.97)  LDD0686  [1]
 LDCM0370  CL101 HEK-293T C323(0.75); C408(1.06)  LDD1574  [2]
 LDCM0374  CL105 HEK-293T C323(0.59); C408(1.02)  LDD1578  [2]
 LDCM0378  CL109 HEK-293T C323(0.54); C408(1.05)  LDD1582  [2]
 LDCM0382  CL112 HCT 116 C408(0.99); C412(0.99)  LDD0699  [1]
 LDCM0383  CL113 HCT 116 C408(1.02); C412(1.02)  LDD0700  [1]
 LDCM0384  CL114 HCT 116 C408(1.04); C412(1.04)  LDD0701  [1]
 LDCM0385  CL115 HCT 116 C408(1.13); C412(1.13)  LDD0702  [1]
 LDCM0386  CL116 HCT 116 C408(1.17); C412(1.17)  LDD0703  [1]
 LDCM0387  CL117 HEK-293T C323(0.55); C408(1.05)  LDD1591  [2]
 LDCM0392  CL121 HEK-293T C323(0.84); C408(1.05)  LDD1596  [2]
 LDCM0396  CL125 HEK-293T C323(0.58); C408(1.04)  LDD1600  [2]
 LDCM0400  CL13 HEK-293T C323(0.72); C408(1.03)  LDD1604  [2]
 LDCM0413  CL25 HEK-293T C323(0.70); C408(0.99)  LDD1617  [2]
 LDCM0426  CL37 HEK-293T C323(0.62); C408(0.95)  LDD1630  [2]
 LDCM0439  CL49 HEK-293T C323(0.54); C408(1.22)  LDD1643  [2]
 LDCM0453  CL61 HEK-293T C323(0.58); C408(1.09)  LDD1656  [2]
 LDCM0466  CL73 HEK-293T C323(0.81); C408(1.07)  LDD1669  [2]
 LDCM0479  CL85 HEK-293T C323(0.92); C408(0.99)  LDD1682  [2]
 LDCM0486  CL91 HCT 116 C408(1.47); C412(1.47)  LDD0803  [1]
 LDCM0487  CL92 HCT 116 C408(1.08); C412(1.08)  LDD0804  [1]
 LDCM0488  CL93 HCT 116 C408(1.04); C412(1.04)  LDD0805  [1]
 LDCM0489  CL94 HCT 116 C408(0.95); C412(0.95)  LDD0806  [1]
 LDCM0490  CL95 HCT 116 C408(1.01); C412(1.01)  LDD0807  [1]
 LDCM0491  CL96 HCT 116 C408(1.05); C412(1.05)  LDD0808  [1]
 LDCM0492  CL97 HCT 116 C408(0.99); C412(0.99)  LDD0809  [1]
 LDCM0493  CL98 HCT 116 C408(1.09); C412(1.09)  LDD0810  [1]
 LDCM0494  CL99 HCT 116 C408(1.42); C412(1.42)  LDD0811  [1]
 LDCM0022  KB02 HEK-293T C323(0.82)  LDD1492  [2]
 LDCM0023  KB03 HEK-293T C323(0.87)  LDD1497  [2]
 LDCM0024  KB05 SK-MEL-28 C323(1.34); C115(2.44)  LDD3427  [3]

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840