Details of the Target

General Information of Target

Target ID LDTP13385
Target Name Ras GTPase-activating protein nGAP (RASAL2)
Gene Name RASAL2
Gene ID 9462
Synonyms
NGAP; Ras GTPase-activating protein nGAP; RAS protein activator-like 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MFNGEPGPASSGASRNVVRSSSISGEICGSQQAGGGAGTTTAKKRRSSLGAKMVAIVGLT
QWSKSTLQLPQPEGATKKLRSNIRRSTETGIAVEMRSRVTRQGSRESTDGSTNSNSSDGT
FIFPTTRLGAESQFSDFLDGLGPAQIVGRQTLATPPMGDVHIAIMDRSGQLEVEVIEARG
LTPKPGSKSLPATYIKVYLLENGACLAKKKTKMTKKTCDPLYQQALLFDEGPQGKVLQVI
VWGDYGRMDHKCFMGMAQIMLDELDLSAAVTGWYKLFPTSSVADSTLGSLTRRLSQSSLE
SATSPSCS
Target Bioclass
Other
Function Inhibitory regulator of the Ras-cyclic AMP pathway.
Uniprot ID
Q9UJF2
Ensemble ID
ENST00000367649.8
HGNC ID
HGNC:9874

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K1029(0.77); K1107(5.51)  LDD0277  [1]
DBIA
 Probe Info  		C508(2.16); C591(3.64)  LDD3311  [2]
NAIA_5
 Probe Info  		C443(20.00)  LDD2227  [3]
AHL-Pu-1
 Probe Info  		C97(3.36)  LDD0170  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [5]
IPM
 Probe Info  		C164(0.00); C443(0.00)  LDD0147  [6]
TFBX
 Probe Info  		C164(0.00); C443(0.00)  LDD0148  [6]
AOyne
 Probe Info  		13.60  LDD0443  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA DM93 C97(3.36)  LDD0170  [4]
 LDCM0026  4SU-RNA+native RNA DM93 C97(4.10)  LDD0171  [4]
 LDCM0632  CL-Sc Hep-G2 C443(20.00)  LDD2227  [3]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C460(2.63); C415(1.61)  LDD1702  [8]
 LDCM0022  KB02 786-O C508(1.73); C591(2.23)  LDD2247  [2]
 LDCM0023  KB03 769-P C508(2.70)  LDD2663  [2]
 LDCM0024  KB05 G361 C508(2.16); C591(3.64)  LDD3311  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Melanoma inhibitory activity protein 2 (MIA2) MIA/OTOR family Q96PC5
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Colorectal mutant cancer protein (MCC) MCC family P23508
Harmonin-binding protein USHBP1 (USHBP1) MCC family Q8N6Y0
TNF receptor-associated factor 1 (TRAF1) . Q13077

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
6 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
7 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
8 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761