Details of the Target

General Information of Target

Target ID LDTP13380
Target Name DNA helicase MCM8 (MCM8)
Gene Name MCM8
Gene ID 84515
Synonyms
C20orf154; DNA helicase MCM8; EC 3.6.4.12; Minichromosome maintenance 8
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLALRVARGSWGALRGAAWAPGTRPSKRRACWALLPPVPCCLGCLAERWRLRPAALGLRL
PGIGQRNHCSGAGKAAPRPAAGAGAAAEAPGGQWGPASTPSLYENPWTIPNMLSMTRIGL
APVLGYLIIEEDFNIALGVFALAGLTDLLDGFIARNWANQRSALGSALDPLADKILISIL
YVSLTYADLIPVPLTYMIISRDVMLIAAVFYVRYRTLPTPRTLAKYFNPCYATARLKPTF
ISKVNTAVQLILVAASLAAPVFNYADSIYLQILWCFTAFTTAASAYSYYHYGRKTVQVIK
D
Target Bioclass
Enzyme
Family
MCM family
Subcellular location
Nucleus
Function
Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR). Required for DNA resection by the MRE11-RAD50-NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity. Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs. The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression. However, may play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC). Probably by regulating HR, plays a key role during gametogenesis. Stabilizes MCM9 protein.
Uniprot ID
Q9UJA3
Ensemble ID
ENST00000265187.4
HGNC ID
HGNC:16147

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
DU145 SNV: p.G271V; p.Q536H .
HT Deletion: p.K101NfsTer15 .
HT115 SNV: p.A154T .
ICC4 SNV: p.G180R .
JURKAT SNV: p.Q162Ter .
LS180 SNV: p.L221P; p.P281T; p.T488K .
MOLT4 SNV: p.E656Ter .
NALM6 SNV: p.L827H .
NCIH1944 SNV: p.D39G .
NCIN87 SNV: p.Q701E .
SKMEL30 SNV: p.G29E .
TOV21G SNV: p.G831D .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C588(5.49)  LDD3373  [1]
IA-alkyne
 Probe Info  		C155(0.00); C235(0.00)  LDD0165  [2]
IPM
 Probe Info  		C411(0.00); C235(0.00); C468(0.00)  LDD2156  [3]
TFBX
 Probe Info  		N.A.  LDD0148  [4]
AOyne
 Probe Info  		15.00  LDD0443  [5]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C153
 Probe Info  		15.35  LDD1834  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0022  KB02 A2058 C588(1.68)  LDD2253  [1]
 LDCM0023  KB03 A2058 C588(3.49)  LDD2670  [1]
 LDCM0024  KB05 OCUG-1 C588(5.49)  LDD3373  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
DNA helicase MCM9 (MCM9) MCM family Q9NXL9
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein fem-1 homolog C (FEM1C) Fem-1 family Q96JP0
Mini-chromosome maintenance complex-binding protein (MCMBP) MCMBP family Q9BTE3

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
4 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
5 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
6 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587