Details of the Target

General Information of Target

Target ID LDTP12675
Target Name Ethanolamine kinase 2 (ETNK2)
Gene Name ETNK2
Gene ID 55224
Synonyms
EKI2; Ethanolamine kinase 2; EKI 2; EC 2.7.1.82; Ethanolamine kinase-like protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAAAAEERMAEEGGGGQGDGGSSLASGSTQRQPPPPAPQHPQPGSQALPAPALAPDQLPQ
NNTLVALPIVAIENILSFMSYDEISQLRLVCKRMDLVCQRMLNQGFLKVERYHNLCQKQV
KAQLPRRESERRNHSLARHADILAAVETRLSLLNMTFMKYVDSNLCCFIPGKVIDEIYRV
LRYVNSTRAPQRAHEVLQELRDISSMAMEYFDEKIVPILKRKLPGSDVSGRLMGSPPVPG
PSAALTTMQLFSKQNPSRQEVTKLQQQVKTNGAGVTVLRREISELRTKVQEQQKQLQDQD
QKLLEQTQIIGEQNARLAELERKLREVMESAVGNSSGSGQNEESPRKRKKATEAIDSLRK
SKRLRNRK
Target Bioclass
Enzyme
Family
Choline/ethanolamine kinase family
Function Highly specific for ethanolamine phosphorylation. Does not have choline kinase activity.
Uniprot ID
Q9NVF9
Ensemble ID
ENST00000367201.7
HGNC ID
HGNC:25575

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
JVM3 SNV: p.W27L .
LS123 Deletion: p.Y311TfsTer11 .
MOLT4 SNV: p.G282D IA-alkyne    Probe Info 
SNU1 SNV: p.A2D DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		N.A.  LDD0241  [1]
DBIA
 Probe Info  		C39(1.34)  LDD3318  [2]
Backus_15
 Probe Info  		2.01  LDD0423  [3]
IA-alkyne
 Probe Info  		C99(0.00); C39(0.00); C139(0.00)  LDD0165  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C39(1.75)  LDD1702  [5]
 LDCM0167  HOIPIN 11a HEK-293T 2.01  LDD0423  [3]
 LDCM0022  KB02 769-P C39(1.58)  LDD2246  [2]
 LDCM0023  KB03 Jurkat C39(10.75)  LDD0315  [4]
 LDCM0024  KB05 MELHO C39(1.34)  LDD3318  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Syntaxin-1A (STX1A) Syntaxin family Q16623
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cysteine-rich tail protein 1 (CYSRT1) CYSRT1 family A8MQ03
Keratin-associated protein 12-2 (KRTAP12-2) KRTAP type 12 family P59991

References

1 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Fragment-Based Covalent Ligand Screening Enables Rapid Discovery of Inhibitors for the RBR E3 Ubiquitin Ligase HOIP. J Am Chem Soc. 2019 Feb 13;141(6):2703-2712. doi: 10.1021/jacs.8b13193. Epub 2019 Feb 4.
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761