Details of the Target

General Information of Target

Target ID LDTP12045
Target Name tRNA N(3)-methylcytidine methyltransferase METTL8, mitochondrial (METTL8)
Gene Name METTL8
Gene ID 79828
Synonyms
tRNA N(3)-methylcytidine methyltransferase METTL8, mitochondrial; EC 2.1.1.-; Methyltransferase-like protein 8; mRNA N(3)-methylcytidine methyltransferase METTL8; EC 2.1.1.-
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MNGVLIPHTPIAVDFWSLRRAGTARLFFLSHMHSDHTVGLSSTWARPLYCSPITAHLLHR
HLQVSKQWIQALEVGESHVLPLDEIGQETMTVTLLDANHCPGSVMFLFEGYFGTILYTGD
FRYTPSMLKEPALTLGKQIHTLYLDNTNCNPALVLPSRQEAAHQIVQLIRKHPQHNIKIG
LYSLGKESLLEQLALEFQTWVVLSPRRLELVQLLGLADVFTVEEKAGRIHAVDHMEICHS
NMLRWNQTHPTIAILPTSRKIHSSHPDIHVIPYSDHSSYSELRAFVAALKPCQVVPIVSR
RPCGGFQDSLSPRISVPLIPDSVQQYMSSSSRKPSLLWLLERRLKRPRTQGVVFESPEES
ADQSQADRDSKKAKKEKLSPWPADLEKQPSHHPLRIKKQLFPDLYSKEWNKAVPFCESQK
RVTMLTAPLGFSVHLRSTDEEFISQKTREEIGLGSPLVPMGDDDGGPEATGNQSAWMGHG
SPLSHSSKGTPLLATEFRGLALKYLLTPVNFFQAGYSSRRFDQQVEKYHKPC
Target Bioclass
Enzyme
Family
Methyltransferase superfamily, METL family
Subcellular location
Mitochondrion
Function
Mitochondrial S-adenosyl-L-methionine-dependent methyltransferase that mediates N(3)-methylcytidine modification of residue 32 of the tRNA anticodon loop of mitochondrial tRNA(Ser)(UCN) and tRNA(Thr). N(3)-methylcytidine methylation modification regulates mitochondrial translation efficiency and is required for activity of the respiratory chain. N(3)-methylcytidine methylation of mitochondrial tRNA(Ser)(UCN) requires the formation of N(6)-dimethylallyladenosine(37) (i6A37) by TRIT1 as prerequisite. May also mediate N(3)-methylcytidine modification of mRNAs. The existence of N(3)-methylcytidine modification on mRNAs is however unclear, and additional evidences are required to confirm the role of the N(3)-methylcytidine-specific mRNA methyltransferase activity of METTL8 in vivo.
Uniprot ID
Q9H825
Ensemble ID
ENST00000392604.6
HGNC ID
HGNC:25856

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		N.A.  LDD0241  [1]
DBIA
 Probe Info  		C96(2.07)  LDD3364  [2]
AHL-Pu-1
 Probe Info  		C146(2.14)  LDD0171  [3]
IA-alkyne
 Probe Info  		C146(0.44)  LDD2183  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0026  4SU-RNA+native RNA DM93 C146(2.14)  LDD0171  [3]
 LDCM0576  Fragment14 Ramos C146(0.25)  LDD2193  [4]
 LDCM0586  Fragment28 Ramos C146(0.43)  LDD2198  [4]
 LDCM0566  Fragment4 Ramos C146(1.76)  LDD2184  [4]
 LDCM0569  Fragment7 Ramos C146(0.98)  LDD2186  [4]
 LDCM0022  KB02 AGS C96(2.12)  LDD2263  [2]
 LDCM0023  KB03 Ramos C146(0.44)  LDD2183  [4]
 LDCM0024  KB05 NCI-N87 C96(2.07)  LDD3364  [2]

References

1 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
4 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578