Details of the Target

General Information of Target

Target ID LDTP11789
Target Name Cytosolic beta-glucosidase (GBA3)
Gene Name GBA3
Gene ID 57733
Synonyms
CBG; CBGL1; Cytosolic beta-glucosidase; EC 3.2.1.21; Cytosolic beta-glucosidase-like protein 1; Cytosolic galactosylceramidase; EC 3.2.1.46; Cytosolic glucosylceramidase; EC 3.2.1.45; Cytosolic glycosylceramidase; Cytosolic GCase; Glucosidase beta acid 3; Glucosylceramidase beta 3; Klotho-related protein; KLrP
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MFSWMGRQAGGRERAGGADAVQTVTGGLRSLYLRKVLPLEEAYRFHEFHSPALEDADFEN
KPMILLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMYGETEGSTPGNALVVD
PKKPFRKLSRFGNAFLNRFMCSQLPNQVLKSISVIDSPGILSGEKQRISRGYDFCQVLQW
FAERVDRIILLFDAHKLDISDEFSEAIKAFRGQDDKIRVVLNKADQVDTQQLMRVYGALM
WSLGKVINTPEVLRVYIGSFWAQPLQNTDNRRLFEAEAQDLFRDIQSLPQKAAVRKLNDL
IKRARLAKVHAYIISYLKKEMPSVFGKENKKRELISRLPEIYIQLQREYQISAGDFPEVK
AMQEQLENYDFTKFHSLKPKLIEAVDNMLSNKISPLMNLISQEETSTPTQLVQGGAFDGT
TEGPFNQGYGEGAKEGADEEEWVVAKDKPVYDELFYTLSPINGKISGVNAKKEMVTSKLP
NSVLGKIWKLADCDCDGMLDEEEFALAKHLIKIKLDGYELPSSLPPHLVPPSHRKSLPKA
D
Target Bioclass
Enzyme
Family
Glycosyl hydrolase 1 family, Klotho subfamily
Subcellular location
Cytoplasm, cytosol
Function
Neutral cytosolic beta-glycosidase with a broad substrate specificity that could play a role in the catabolism of glycosylceramides . Has a significant glucosylceramidase activity in vitro. However, that activity is relatively low and its significance in vivo is not clear. Hydrolyzes galactosylceramides/GalCers, glucosylsphingosines/GlcSphs and galactosylsphingosines/GalSphs. However, the in vivo relevance of these activities is unclear. It can also hydrolyze a broad variety of dietary glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens in vitro and could therefore play a role in the metabolism of xenobiotics. Possesses transxylosylase activity in vitro using xylosylated ceramides/XylCers (such as beta-D-xylosyl-(1<->1')-N-acylsphing-4-enine) as xylosyl donors and cholesterol as acceptor. Could also play a role in the catabolism of cytosolic sialyl free N-glycans.
Uniprot ID
Q9H227
Ensemble ID
ENST00000709220.1
HGNC ID
HGNC:19069
ChEMBL ID
CHEMBL3865

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
BTD
 Probe Info  		C30(0.34)  LDD2106  [1]
IPM
 Probe Info  		C30(1.68)  LDD1701  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C30(0.35)  LDD2112  [1]
 LDCM0023  KB03 MDA-MB-231 C30(1.68)  LDD1701  [1]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C30(0.34)  LDD2106  [1]
 LDCM0517  Nucleophilic fragment 21b MDA-MB-231 C30(0.36)  LDD2110  [1]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C30(0.37)  LDD2114  [1]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C30(0.20)  LDD2145  [1]

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761