Details of the Target

General Information of Target

Target ID LDTP11677
Target Name Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP)
Gene Name LHPP
Gene ID 64077
Synonyms
Phospholysine phosphohistidine inorganic pyrophosphate phosphatase; hLHPP; EC 3.1.3.-; EC 3.6.1.1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSTKQITCRYFMHGVCREGSQCLFSHDLANSKPSTICKYYQKGYCAYGTRCRYDHTRPSA
AAGGAVGTMAHSVPSPAFHSPHPPSEVTASIVKTNSHEPGKREKRTLVLRDRNLSGMAER
KTQPSMVSNPGSCSDPQPSPEMKPHSYLDAIRSGLDDVEASSSYSNEQQLCPYAAAGECR
FGDACVYLHGEVCEICRLQVLHPFDPEQRKAHEKICMLTFEHEMEKAFAFQASQDKVCSI
CMEVILEKASASERRFGILSNCNHTYCLSCIRQWRCAKQFENPIIKSCPECRVISEFVIP
SVYWVEDQNKKNELIEAFKQGMGKKACKYFEQGKGTCPFGSKCLYRHAYPDGRLAEPEKP
RKQLSSQGTVRFFNSVRLWDFIENRESRHVPNNEDVDMTELGDLFMHLSGVESSEP
Target Bioclass
Enzyme
Family
HAD-like hydrolase superfamily
Subcellular location
Cytoplasm
Function Phosphatase that hydrolyzes imidodiphosphate, 3-phosphohistidine and 6-phospholysine. Has broad substrate specificity and can also hydrolyze inorganic diphosphate, but with lower efficiency.
Uniprot ID
Q9H008
Ensemble ID
ENST00000368839.1
HGNC ID
HGNC:30042
ChEMBL ID
CHEMBL5169196

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A498 SNV: p.M211I .
DU145 SNV: p.G72Ter .
MFE319 SNV: p.M1? .
NCIH1944 SNV: p.L142F .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C53(2.21)  LDD3382  [1]
4-Iodoacetamidophenylacetylene
 Probe Info  		C180(0.00); C53(0.00); C88(0.00)  LDD0038  [2]
IA-alkyne
 Probe Info  		C180(0.00); C88(0.00)  LDD0036  [2]
Lodoacetamide azide
 Probe Info  		C180(0.00); C53(0.00); C88(0.00)  LDD0037  [2]
NAIA_5
 Probe Info  		N.A.  LDD2223  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C88(1.08); C53(1.01)  LDD1508  [4]
 LDCM0237  AC12 HEK-293T C88(0.93)  LDD1510  [4]
 LDCM0277  AC18 HEK-293T C88(1.07); C53(0.88)  LDD1516  [4]
 LDCM0279  AC2 HEK-293T C88(1.09); C53(0.89)  LDD1518  [4]
 LDCM0280  AC20 HEK-293T C88(0.82)  LDD1519  [4]
 LDCM0286  AC26 HEK-293T C88(1.07); C53(0.83)  LDD1525  [4]
 LDCM0288  AC28 HEK-293T C88(0.78)  LDD1527  [4]
 LDCM0295  AC34 HEK-293T C88(0.97); C53(0.99)  LDD1534  [4]
 LDCM0297  AC36 HEK-293T C88(0.95)  LDD1536  [4]
 LDCM0301  AC4 HEK-293T C88(0.92)  LDD1540  [4]
 LDCM0304  AC42 HEK-293T C88(1.13); C53(0.90)  LDD1543  [4]
 LDCM0306  AC44 HEK-293T C88(1.02)  LDD1545  [4]
 LDCM0313  AC50 HEK-293T C88(1.07); C53(1.04)  LDD1552  [4]
 LDCM0315  AC52 HEK-293T C88(0.95)  LDD1554  [4]
 LDCM0321  AC58 HEK-293T C88(1.18); C53(1.06)  LDD1560  [4]
 LDCM0324  AC60 HEK-293T C88(0.79)  LDD1563  [4]
 LDCM0405  CL18 HEK-293T C88(1.16); C53(1.11)  LDD1609  [4]
 LDCM0408  CL20 HEK-293T C88(0.91)  LDD1612  [4]
 LDCM0419  CL30 HEK-293T C88(1.05); C53(1.05)  LDD1623  [4]
 LDCM0421  CL32 HEK-293T C88(1.02)  LDD1625  [4]
 LDCM0432  CL42 HEK-293T C88(1.01); C53(0.93)  LDD1636  [4]
 LDCM0434  CL44 HEK-293T C88(0.75)  LDD1638  [4]
 LDCM0445  CL54 HEK-293T C88(1.30); C53(1.09)  LDD1648  [4]
 LDCM0447  CL56 HEK-293T C88(0.87)  LDD1650  [4]
 LDCM0451  CL6 HEK-293T C88(1.15); C53(0.93)  LDD1654  [4]
 LDCM0458  CL66 HEK-293T C88(1.35); C53(1.17)  LDD1661  [4]
 LDCM0460  CL68 HEK-293T C88(1.06)  LDD1663  [4]
 LDCM0471  CL78 HEK-293T C88(1.12); C53(1.00)  LDD1674  [4]
 LDCM0473  CL8 HEK-293T C88(0.73)  LDD1676  [4]
 LDCM0474  CL80 HEK-293T C88(1.11)  LDD1677  [4]
 LDCM0485  CL90 HEK-293T C88(0.97); C53(0.92)  LDD1688  [4]
 LDCM0487  CL92 HEK-293T C88(0.82)  LDD1690  [4]
 LDCM0022  KB02 HEK-293T C88(0.98)  LDD1492  [4]
 LDCM0023  KB03 HEK-293T C88(0.98)  LDD1497  [4]
 LDCM0024  KB05 OVCAR-4 C53(2.21)  LDD3382  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger and SCAN domain-containing protein 23 (ZSCAN23) Krueppel C2H2-type zinc-finger protein family Q3MJ62

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402