Details of the Target

General Information of Target

Target ID LDTP11653
Target Name Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 (CTDSP1)
Gene Name CTDSP1
Gene ID 58190
Synonyms
NIF3; NLIIF; SCP1; Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1; EC 3.1.3.16; Nuclear LIM interactor-interacting factor 3; NLI-IF; NLI-interacting factor 3; Small C-terminal domain phosphatase 1; SCP1; Small CTD phosphatase 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLVLLLHAVVLGLPSAWAVGACARACPAACACSTVERGCSVRCDRAGLLRVPAELPCEAV
SIDLDRNGLRFLGERAFGTLPSLRRLSLRHNNLSFITPGAFKGLPRLAELRLAHNGDLRY
LHARTFAALSRLRRLDLAACRLFSVPERLLAELPALRELAAFDNLFRRVPGALRGLANLT
HAHLERGRIEAVASSSLQGLRRLRSLSLQANRVRAVHAGAFGDCGVLEHLLLNDNLLAEL
PADAFRGLRRLRTLNLGGNALDRVARAWFADLAELELLYLDRNSIAFVEEGAFQNLSGLL
ALHLNGNRLTVLAWVAFQPGFFLGRLFLFRNPWCCDCRLEWLRDWMEGSGRVTDVPCASP
GSVAGLDLSQVTFGRSSDGLCVDPEELNLTTSSPGPSPEPAATTVSRFSSLLSKLLAPRV
PVEEAANTTGGLANASLSDSLSSRGVGGAGRQPWFLLASCLLPSVAQHVVFGLQMD
Target Bioclass
Enzyme
Subcellular location
Nucleus
Function
Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells.
Uniprot ID
Q9GZU7
Ensemble ID
ENST00000273062.7
HGNC ID
HGNC:21614
ChEMBL ID
CHEMBL1795098

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCSW1 SNV: p.E242D .
HCT15 SNV: p.Y78C .
HEC1B SNV: p.R175Q .
JURKAT SNV: p.V127I .
LNCaP clone FGC SNV: p.S193C .
NCIH1793 SNV: p.F241V .
NCIH2286 SNV: p.F114L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		N.A.  LDD0241  [1]
DBIA
 Probe Info  		C45(0.98)  LDD1509  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0226  AC11 HEK-293T C45(0.98)  LDD1509  [2]
 LDCM0270  AC15 HEK-293T C45(0.91)  LDD1513  [2]
 LDCM0278  AC19 HEK-293T C45(0.93)  LDD1517  [2]
 LDCM0281  AC21 HEK-293T C45(1.07)  LDD1520  [2]
 LDCM0283  AC23 HEK-293T C45(0.99)  LDD1522  [2]
 LDCM0287  AC27 HEK-293T C45(1.02)  LDD1526  [2]
 LDCM0289  AC29 HEK-293T C45(0.90)  LDD1528  [2]
 LDCM0290  AC3 HEK-293T C45(0.94)  LDD1529  [2]
 LDCM0292  AC31 HEK-293T C45(0.95)  LDD1531  [2]
 LDCM0296  AC35 HEK-293T C45(0.91)  LDD1535  [2]
 LDCM0298  AC37 HEK-293T C45(0.92)  LDD1537  [2]
 LDCM0300  AC39 HEK-293T C45(0.95)  LDD1539  [2]
 LDCM0305  AC43 HEK-293T C45(0.97)  LDD1544  [2]
 LDCM0307  AC45 HEK-293T C45(0.97)  LDD1546  [2]
 LDCM0309  AC47 HEK-293T C45(0.85)  LDD1548  [2]
 LDCM0312  AC5 HEK-293T C45(0.99)  LDD1551  [2]
 LDCM0314  AC51 HEK-293T C45(0.97)  LDD1553  [2]
 LDCM0316  AC53 HEK-293T C45(0.96)  LDD1555  [2]
 LDCM0318  AC55 HEK-293T C45(0.88)  LDD1557  [2]
 LDCM0322  AC59 HEK-293T C45(0.96)  LDD1561  [2]
 LDCM0325  AC61 HEK-293T C45(0.85)  LDD1564  [2]
 LDCM0327  AC63 HEK-293T C45(0.98)  LDD1566  [2]
 LDCM0334  AC7 HEK-293T C45(0.96)  LDD1568  [2]
 LDCM0248  AKOS034007472 HEK-293T C45(0.90)  LDD1511  [2]
 LDCM0367  CL1 HEK-293T C45(0.82)  LDD1571  [2]
 LDCM0370  CL101 HEK-293T C45(1.09)  LDD1574  [2]
 LDCM0374  CL105 HEK-293T C45(0.89)  LDD1578  [2]
 LDCM0378  CL109 HEK-293T C45(0.89)  LDD1582  [2]
 LDCM0379  CL11 HEK-293T C45(0.98)  LDD1583  [2]
 LDCM0383  CL113 HEK-293T C45(0.87)  LDD1587  [2]
 LDCM0387  CL117 HEK-293T C45(0.94)  LDD1591  [2]
 LDCM0392  CL121 HEK-293T C45(0.91)  LDD1596  [2]
 LDCM0396  CL125 HEK-293T C45(0.83)  LDD1600  [2]
 LDCM0400  CL13 HEK-293T C45(1.05)  LDD1604  [2]
 LDCM0406  CL19 HEK-293T C45(1.06)  LDD1610  [2]
 LDCM0409  CL21 HEK-293T C45(0.93)  LDD1613  [2]
 LDCM0411  CL23 HEK-293T C45(0.97)  LDD1615  [2]
 LDCM0413  CL25 HEK-293T C45(1.16)  LDD1617  [2]
 LDCM0420  CL31 HEK-293T C45(0.96)  LDD1624  [2]
 LDCM0422  CL33 HEK-293T C45(0.51)  LDD1626  [2]
 LDCM0424  CL35 HEK-293T C45(0.87)  LDD1628  [2]
 LDCM0426  CL37 HEK-293T C45(0.99)  LDD1630  [2]
 LDCM0433  CL43 HEK-293T C45(0.94)  LDD1637  [2]
 LDCM0435  CL45 HEK-293T C45(0.87)  LDD1639  [2]
 LDCM0437  CL47 HEK-293T C45(0.92)  LDD1641  [2]
 LDCM0439  CL49 HEK-293T C45(0.90)  LDD1643  [2]
 LDCM0446  CL55 HEK-293T C45(0.92)  LDD1649  [2]
 LDCM0448  CL57 HEK-293T C45(0.56)  LDD1651  [2]
 LDCM0450  CL59 HEK-293T C45(1.00)  LDD1653  [2]
 LDCM0453  CL61 HEK-293T C45(0.81)  LDD1656  [2]
 LDCM0459  CL67 HEK-293T C45(1.17)  LDD1662  [2]
 LDCM0461  CL69 HEK-293T C45(1.01)  LDD1664  [2]
 LDCM0462  CL7 HEK-293T C45(1.05)  LDD1665  [2]
 LDCM0464  CL71 HEK-293T C45(0.74)  LDD1667  [2]
 LDCM0466  CL73 HEK-293T C45(0.94)  LDD1669  [2]
 LDCM0472  CL79 HEK-293T C45(0.93)  LDD1675  [2]
 LDCM0475  CL81 HEK-293T C45(0.90)  LDD1678  [2]
 LDCM0477  CL83 HEK-293T C45(0.88)  LDD1680  [2]
 LDCM0479  CL85 HEK-293T C45(0.96)  LDD1682  [2]
 LDCM0484  CL9 HEK-293T C45(0.89)  LDD1687  [2]
 LDCM0486  CL91 HEK-293T C45(0.89)  LDD1689  [2]
 LDCM0488  CL93 HEK-293T C45(0.89)  LDD1691  [2]
 LDCM0490  CL95 HEK-293T C45(1.00)  LDD1693  [2]
 LDCM0492  CL97 HEK-293T C45(0.94)  LDD1695  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Inositol-trisphosphate 3-kinase B (ITPKB) Inositol phosphokinase (IPK) family P27987
Protein yippee-like 5 (YPEL5) Yippee family P62699
E3 ubiquitin-protein ligase FANCL (FANCL) . Q9NW38
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Sodium channel modifier 1 (SCNM1) . Q9BWG6
Syntenin-1 (SDCBP) . O00560
Transcription factor
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mothers against decapentaplegic homolog 1 (SMAD1) Dwarfin/SMAD family Q15797
Zinc finger and BTB domain-containing protein 16 (ZBTB16) Krueppel C2H2-type zinc-finger protein family Q05516
Other
Click To Hide/Show 14 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Alpha-actinin-3 (ACTN3) Alpha-actinin family Q08043
Beta-crystallin A2 (CRYBA2) Beta/gamma-crystallin family P53672
Cyclin-dependent kinase 4 inhibitor C (CDKN2C) CDKN2 cyclin-dependent kinase inhibitor family P42773
Protein FAM110A (FAM110A) FAM110 family Q9BQ89
Myelin basic protein (MBP) Myelin basic protein family P02686
Paraneoplastic antigen Ma1 (PNMA1) PNMA family Q8ND90
Guanine nucleotide exchange factor for Rab-3A (RAB3IL1) SEC2 family Q8TBN0
Tektin-4 (TEKT4) Tektin family Q8WW24
BAG family molecular chaperone regulator 3 (BAG3) . O95817
Cell division cycle-associated protein 3 (CDCA3) . Q99618
Cleavage stimulation factor subunit 2 tau variant (CSTF2T) . Q9H0L4
Mitogen-activated protein kinase-binding protein 1 (MAPKBP1) . O60336
MORN repeat-containing protein 3 (MORN3) . Q6PF18
Stimulated by retinoic acid gene 8 protein homolog (STRA8) . Q7Z7C7

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Citric Acid Small molecular drug DB04272
Investigative
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Aspartate Beryllium Trifluoride . DB04156

References

1 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402