Details of the Target

General Information of Target

Target ID LDTP11404
Target Name Collagen alpha-1(XXV) chain (COL25A1)
Gene Name COL25A1
Gene ID 84570
Synonyms
Collagen alpha-1(XXV) chain; Alzheimer disease amyloid-associated protein; AMY; CLAC-P) [Cleaved into: Collagen-like Alzheimer amyloid plaque component; CLAC)]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLLLFSVILISWVSTVGGEGTLCDFPKIHHGFLYDEEDYNPFSQVPTGEVFYYSCEYNFV
SPSKSFWTRITCTEEGWSPTPKCLRMCSFPFVKNGHSESSGLIHLEGDTVQIICNTGYSL
QNNEKNISCVERGWSTPPICSFTKGECHVPILEANVDAQPKKESYKVGDVLKFSCRKNLI
RVGSDSVQCYQFGWSPNFPTCKGQVRSCGPPPQLSNGEVKEIRKEEYGHNEVVEYDCNPN
FIINGPKKIQCVDGEWTTLPTCVEQVKTCGYIPELEYGYVQPSVPPYQHGVSVEVNCRNE
YAMIGNNMITCINGIWTELPMCVATHQLKRCKIAGVNIKTLLKLSGKEFNHNSRIRYRCS
DIFRYRHSVCINGKWNPEVDCTEKREQFCPPPPQIPNAQNMTTTVNYQDGEKVAVLCKEN
YLLPEAKEIVCKDGRWQSLPRCVESTAYCGPPPSINNGDTTSFPLSVYPPGSTVTYRCQS
FYKLQGSVTVTCRNKQWSEPPRCLDPCVVSEENMNKNNIQLKWRNDGKLYAKTGDAVEFQ
CKFPHKAMISSPPFRAICQEGKFEYPICE
Target Bioclass
Other
Subcellular location
Membrane
Function Inhibits fibrillization of amyloid-beta peptide during the elongation phase. Has also been shown to assemble amyloid fibrils into protease-resistant aggregates. Binds heparin.
Uniprot ID
Q9BXS0
Ensemble ID
ENST00000399126.1
HGNC ID
HGNC:18603

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.L40F; p.R95Ter .
DU145 SNV: p.G515V .
HCC15 SNV: p.P22T .
HCT15 SNV: p.I429M .
HEC1 SNV: p.G371Ter .
HT115 SNV: p.A637V .
MDAMB468 SNV: p.V89G .
NB1 SNV: p.G479Ter .
OVCAR8 SNV: p.G464V .
SW1271 SNV: p.L50Q .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C27(0.87)  LDD1492  [1]
TFBX
 Probe Info  		N.A.  LDD0027  [2]
IPM
 Probe Info  		N.A.  LDD2156  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0237  AC12 HEK-293T C27(1.47)  LDD1510  [1]
 LDCM0280  AC20 HEK-293T C27(1.62)  LDD1519  [1]
 LDCM0288  AC28 HEK-293T C27(1.50)  LDD1527  [1]
 LDCM0297  AC36 HEK-293T C27(1.39)  LDD1536  [1]
 LDCM0301  AC4 HEK-293T C27(1.15)  LDD1540  [1]
 LDCM0306  AC44 HEK-293T C27(1.37)  LDD1545  [1]
 LDCM0315  AC52 HEK-293T C27(1.22)  LDD1554  [1]
 LDCM0324  AC60 HEK-293T C27(1.49)  LDD1563  [1]
 LDCM0408  CL20 HEK-293T C27(1.28)  LDD1612  [1]
 LDCM0421  CL32 HEK-293T C27(1.42)  LDD1625  [1]
 LDCM0434  CL44 HEK-293T C27(1.46)  LDD1638  [1]
 LDCM0447  CL56 HEK-293T C27(1.41)  LDD1650  [1]
 LDCM0460  CL68 HEK-293T C27(1.79)  LDD1663  [1]
 LDCM0473  CL8 HEK-293T C27(2.38)  LDD1676  [1]
 LDCM0474  CL80 HEK-293T C27(1.15)  LDD1677  [1]
 LDCM0487  CL92 HEK-293T C27(1.47)  LDD1690  [1]
 LDCM0022  KB02 HEK-293T C27(0.87)  LDD1492  [1]
 LDCM0023  KB03 HEK-293T C27(0.98)  LDD1497  [1]
 LDCM0024  KB05 HEK-293T C27(0.87)  LDD1502  [1]

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
2 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
3 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019