Details of the Target

General Information of Target

Target ID LDTP11208
Target Name Mediator of RNA polymerase II transcription subunit 10 (MED10)
Gene Name MED10
Gene ID 84246
Synonyms
Mediator of RNA polymerase II transcription subunit 10; Mediator complex subunit 10; Transformation-related gene 17 protein; TRG-17; Transformation-related gene 20 protein; TRG-20
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEMKKKINLELRNRSPEEVTELVLDNCLCVNGEIEGLNDTFKELEFLSMANVELSSLARL
PSLNKLRKLELSDNIISGGLEVLAEKCPNLTYLNLSGNKIKDLSTVEALQNLKNLKSLDL
FNCEITNLEDYRESIFELLQQITYLDGFDQEDNEAPDSEEEDDEDGDEDDEEEEENEAGP
PEGYEEEEEEEEEEDEDEDEDEDEAGSELGEGEEEVGLSYLMKEEIQDEEDDDDYVEEGE
EEEEEEEGGLRGEKRKRDAEDDGEEEDD
Target Bioclass
Other
Family
Mediator complex subunit 10 family
Subcellular location
Nucleus
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Uniprot ID
Q9BTT4
Ensemble ID
ENST00000255764.4
HGNC ID
HGNC:28760

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K106(7.08)  LDD0277  [1]
m-APA
 Probe Info  		N.A.  LDD2233  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [3]
NAIA_4
 Probe Info  		N.A.  LDD2226  [4]
IPM
 Probe Info  		N.A.  LDD0147  [5]
Acrolein
 Probe Info  		N.A.  LDD0217  [6]
NAIA_5
 Probe Info  		N.A.  LDD2223  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [6]
 LDCM0632  CL-Sc Hep-G2 C54(1.20)  LDD2227  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Tissue alpha-L-fucosidase (FUCA1) Glycosyl hydrolase 29 family P04066
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mediator of RNA polymerase II transcription subunit 7 (MED7) Mediator complex subunit 7 family O43513
Rho GDP-dissociation inhibitor 3 (ARHGDIG) Rho GDI family Q99819

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
6 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.