Details of the Target

General Information of Target

Target ID LDTP11128
Target Name Rho GTPase-activating protein 9 (ARHGAP9)
Gene Name ARHGAP9
Gene ID 64333
Synonyms
Rho GTPase-activating protein 9; Rho-type GTPase-activating protein 9
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MALWRGSAYAGFLALAVGCVFLLEPELPGSALRSLWSSLCLGPAPAPPGPVSPEGRLAAA
WDALIVRPVRRWRRVAVGVNACVDVVLSGVKLLQALGLSPGNGKDHSILHSRNDLEEAFI
HFMGKGAAAERFFSDKETFHDIAQVASEFPGAQHYVGGNAALIGQKFAANSDLKVLLCGP
VGPKLHELLDDNVFVPPESLQEVDEFHLILEYQAGEEWGQLKAPHANRFIFSHDLSNGAM
NMLEVFVSSLEEFQPDLVVLSGLHMMEGQSKELQRKRLLEVVTSISDIPTGIPVHLELAS
MTNRELMSSIVHQQVFPAVTSLGLNEQELLFLTQSASGPHSSLSSWNGVPDVGMVSDILF
WILKEHGRSKSRASDLTRIHFHTLVYHILATVDGHWANQLAAVAAGARVAGTQACATETI
DTSRVSLRAPQEFMTSHSEAGSRIVLNPNKPVVEWHREGISFHFTPVLVCKDPIRTVGLG
DAISAEGLFYSEVHPHY
Target Bioclass
Other
Function
GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has a substantial GAP activity toward CDC42 and RAC1 and less toward RHOA. Has a role in regulating adhesion of hematopoietic cells to the extracellular matrix. Binds phosphoinositides, and has the highest affinity for phosphatidylinositol 3,4,5-trisphosphate, followed by phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 4,5-bisphosphate.
Uniprot ID
Q9BRR9
Ensemble ID
ENST00000393791.8
HGNC ID
HGNC:14130

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C562(4.40)  LDD0204  [1]
4-Iodoacetamidophenylacetylene
 Probe Info  		C666(0.00); C562(0.00); C693(0.00)  LDD0038  [2]
IA-alkyne
 Probe Info  		C666(0.00); C693(0.00); C562(0.00)  LDD0036  [2]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [3]
Lodoacetamide azide
 Probe Info  		C562(0.00); C693(0.00); C666(0.00)  LDD0037  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0102  BDHI 8 Jurkat C562(4.40)  LDD0204  [1]
 LDCM0625  F8 Ramos C693(0.67)  LDD2187  [4]
 LDCM0572  Fragment10 Ramos C693(0.23)  LDD2189  [4]
 LDCM0573  Fragment11 Ramos C693(20.00)  LDD2190  [4]
 LDCM0574  Fragment12 Ramos C693(0.43)  LDD2191  [4]
 LDCM0576  Fragment14 Ramos C693(2.00)  LDD2193  [4]
 LDCM0579  Fragment20 Ramos C693(0.60)  LDD2194  [4]
 LDCM0586  Fragment28 Ramos C693(0.75)  LDD2198  [4]
 LDCM0588  Fragment30 Ramos C693(0.31)  LDD2199  [4]
 LDCM0589  Fragment31 Ramos C693(0.60)  LDD2200  [4]
 LDCM0590  Fragment32 Ramos C693(0.19)  LDD2201  [4]
 LDCM0468  Fragment33 Ramos C693(0.41)  LDD2202  [4]
 LDCM0566  Fragment4 Ramos C693(1.19)  LDD2184  [4]
 LDCM0614  Fragment56 Ramos C693(0.49)  LDD2205  [4]
 LDCM0569  Fragment7 Ramos C693(2.91)  LDD2186  [4]
 LDCM0022  KB02 T cell C230(7.30); C239(0.00)  LDD1703  [5]
 LDCM0023  KB03 Ramos C693(1.55)  LDD2183  [4]
 LDCM0024  KB05 NALM-6 C562(2.36)  LDD3339  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Nucleoside diphosphate kinase homolog 7 (NME7) NDK family Q9Y5B8
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
TSC22 domain family protein 4 (TSC22D4) TSC-22/Dip/Bun family Q9Y3Q8
Other
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
ABI gene family member 3 (ABI3) ABI family Q9P2A4
Cysteine-rich tail protein 1 (CYSRT1) CYSRT1 family A8MQ03
Golgin subfamily A member 6-like protein 9 (GOLGA6L9) GOLGA6 family A6NEM1
Golgin subfamily A member 6A (GOLGA6A) GOLGA6 family Q9NYA3
Growth factor receptor-bound protein 2 (GRB2) GRB2/sem-5/DRK family P62993
Large ribosomal subunit protein mL38 (MRPL38) Phosphatidylethanolamine-binding protein family Q96DV4
U1 small nuclear ribonucleoprotein C (SNRPC) U1 small nuclear ribonucleoprotein C family P09234
Four and a half LIM domains protein 2 (FHL2) . Q14192

References

1 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
4 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
5 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
6 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840