Details of the Target

General Information of Target

Target ID LDTP10881
Target Name Eyes absent homolog 1 (EYA1)
Gene Name EYA1
Gene ID 2138
Synonyms
Eyes absent homolog 1; EC 3.1.3.16; EC 3.1.3.48
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MADPVAGIAGSAAKSVRPFRSSEAYVEAMKEDLAEWLNALYGLGLPGGGDGFLTGLATGT
TLCQHANAVTEAARALAAARPARGVAFQAHSVVPGSFMARDNVATFIGWCRVELGVPEVL
MFETEDLVLRKNEKSVVLCLLEVARRGARLGLLAPRLVQFEQEIERELRAAPPAPNAPAA
GEDTTETAPAPGTPARGPRMTPSDLRNLDELVREILGRCTCPDQFPMIKVSEGKYRVGDS
SLLIFVRVLRSHVMVRVGGGWDTLEHYLDKHDPCRCSSTAHRPPQPRVCTFSPQRVSPTT
SPRPASPVPGSERRGSRPEMTPVSLRSTKEGPETPPRPRDQLPPHPRSRRYSGDSDSSAS
SAQSGPLGTRSDDTGTGPRRERPSRRLTTGTPASPRRPPALRSQSRDRLDRGRPRGAPGG
RGAQLSVPSPARRARSQSREEQAVLLVRRDRDGQHSWVPRGRGSGGSGRSTPQTPRARSP
AAPRLSRVSSPSPELGTTPASIFRTPLQLDPQQEQQLFRRLEEEFLANARALEAVASVTP
TGPVPDPARAPDPPAPDSAYCSSSSSSSSLSVLGGKCGQPGDSGRTANGLPGPRSQALSS
SSDEGSPCPGMGGPLDAPGSPLACTEPSRTWARGRMDTQPDRKPSRIPTPRGPRRPSGPA
ELGTWHALHSVTPRAEPDSWM
Target Bioclass
Enzyme
Family
HAD-like hydrolase superfamily, EYA family
Subcellular location
Cytoplasm
Function
Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5. Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Has also phosphatase activity with proteins phosphorylated on Ser and Thr residues (in vitro). Required for normal embryonic development of the craniofacial and trunk skeleton, kidneys and ears. Together with SIX1, it plays an important role in hypaxial muscle development; in this it is functionally redundant with EYA2.
Uniprot ID
Q99502
Ensemble ID
ENST00000340726.8
HGNC ID
HGNC:3519

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
FTC133 SNV: p.P353H .
HCC15 SNV: p.R297G .
HCT15 SNV: p.W327Ter .
IM95 SNV: p.D394V .
JHH1 SNV: p.G70V .
LNCaP clone FGC SNV: p.A237V .
MEWO SNV: p.P315L .
NCIH2286 SNV: p.S278T; p.D579V .
OCILY3 SNV: p.A416T .
OV90 SNV: p.S292C .
OVK18 SNV: p.S181N .
SNGM Deletion: p.L499Ter .
SUPT1 Insertion: p.N377Ter .
SW1271 SNV: p.P93T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C495(0.96)  LDD1507  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C495(0.96)  LDD1507  [1]
 LDCM0226  AC11 HEK-293T C495(1.39)  LDD1509  [1]
 LDCM0270  AC15 HEK-293T C495(1.12)  LDD1513  [1]
 LDCM0276  AC17 HEK-293T C495(1.14)  LDD1515  [1]
 LDCM0278  AC19 HEK-293T C495(1.30)  LDD1517  [1]
 LDCM0283  AC23 HEK-293T C495(0.89)  LDD1522  [1]
 LDCM0285  AC25 HEK-293T C495(1.06)  LDD1524  [1]
 LDCM0287  AC27 HEK-293T C495(1.16)  LDD1526  [1]
 LDCM0290  AC3 HEK-293T C495(1.26)  LDD1529  [1]
 LDCM0292  AC31 HEK-293T C495(0.88)  LDD1531  [1]
 LDCM0294  AC33 HEK-293T C495(0.90)  LDD1533  [1]
 LDCM0296  AC35 HEK-293T C495(1.28)  LDD1535  [1]
 LDCM0300  AC39 HEK-293T C495(1.06)  LDD1539  [1]
 LDCM0303  AC41 HEK-293T C495(1.27)  LDD1542  [1]
 LDCM0305  AC43 HEK-293T C495(1.06)  LDD1544  [1]
 LDCM0309  AC47 HEK-293T C495(1.20)  LDD1548  [1]
 LDCM0311  AC49 HEK-293T C495(1.27)  LDD1550  [1]
 LDCM0314  AC51 HEK-293T C495(1.06)  LDD1553  [1]
 LDCM0318  AC55 HEK-293T C495(1.21)  LDD1557  [1]
 LDCM0320  AC57 HEK-293T C495(1.22)  LDD1559  [1]
 LDCM0322  AC59 HEK-293T C495(1.07)  LDD1561  [1]
 LDCM0327  AC63 HEK-293T C495(1.12)  LDD1566  [1]
 LDCM0334  AC7 HEK-293T C495(0.96)  LDD1568  [1]
 LDCM0356  AKOS034007680 HEK-293T C495(1.00)  LDD1570  [1]
 LDCM0367  CL1 HEK-293T C495(0.82)  LDD1571  [1]
 LDCM0370  CL101 HEK-293T C495(1.05)  LDD1574  [1]
 LDCM0371  CL102 HEK-293T C495(0.90)  LDD1575  [1]
 LDCM0372  CL103 HEK-293T C495(1.15)  LDD1576  [1]
 LDCM0374  CL105 HEK-293T C495(0.93)  LDD1578  [1]
 LDCM0375  CL106 HEK-293T C495(1.13)  LDD1579  [1]
 LDCM0376  CL107 HEK-293T C495(1.22)  LDD1580  [1]
 LDCM0378  CL109 HEK-293T C495(0.95)  LDD1582  [1]
 LDCM0379  CL11 HEK-293T C495(0.86)  LDD1583  [1]
 LDCM0380  CL110 HEK-293T C495(0.86)  LDD1584  [1]
 LDCM0381  CL111 HEK-293T C495(1.01)  LDD1585  [1]
 LDCM0383  CL113 HEK-293T C495(0.99)  LDD1587  [1]
 LDCM0384  CL114 HEK-293T C495(0.94)  LDD1588  [1]
 LDCM0385  CL115 HEK-293T C495(0.90)  LDD1589  [1]
 LDCM0387  CL117 HEK-293T C495(1.08)  LDD1591  [1]
 LDCM0388  CL118 HEK-293T C495(1.30)  LDD1592  [1]
 LDCM0389  CL119 HEK-293T C495(1.27)  LDD1593  [1]
 LDCM0392  CL121 HEK-293T C495(1.15)  LDD1596  [1]
 LDCM0393  CL122 HEK-293T C495(1.12)  LDD1597  [1]
 LDCM0394  CL123 HEK-293T C495(0.93)  LDD1598  [1]
 LDCM0396  CL125 HEK-293T C495(1.16)  LDD1600  [1]
 LDCM0397  CL126 HEK-293T C495(1.03)  LDD1601  [1]
 LDCM0398  CL127 HEK-293T C495(1.30)  LDD1602  [1]
 LDCM0400  CL13 HEK-293T C495(1.12)  LDD1604  [1]
 LDCM0401  CL14 HEK-293T C495(1.02)  LDD1605  [1]
 LDCM0402  CL15 HEK-293T C495(1.34)  LDD1606  [1]
 LDCM0404  CL17 HEK-293T C495(0.99)  LDD1608  [1]
 LDCM0406  CL19 HEK-293T C495(1.27)  LDD1610  [1]
 LDCM0407  CL2 HEK-293T C495(1.04)  LDD1611  [1]
 LDCM0411  CL23 HEK-293T C495(0.92)  LDD1615  [1]
 LDCM0413  CL25 HEK-293T C495(1.34)  LDD1617  [1]
 LDCM0414  CL26 HEK-293T C495(0.98)  LDD1618  [1]
 LDCM0415  CL27 HEK-293T C495(1.24)  LDD1619  [1]
 LDCM0417  CL29 HEK-293T C495(1.12)  LDD1621  [1]
 LDCM0418  CL3 HEK-293T C495(1.26)  LDD1622  [1]
 LDCM0420  CL31 HEK-293T C495(0.99)  LDD1624  [1]
 LDCM0424  CL35 HEK-293T C495(0.99)  LDD1628  [1]
 LDCM0426  CL37 HEK-293T C495(1.17)  LDD1630  [1]
 LDCM0428  CL39 HEK-293T C495(1.16)  LDD1632  [1]
 LDCM0431  CL41 HEK-293T C495(0.97)  LDD1635  [1]
 LDCM0433  CL43 HEK-293T C495(1.53)  LDD1637  [1]
 LDCM0437  CL47 HEK-293T C495(0.88)  LDD1641  [1]
 LDCM0439  CL49 HEK-293T C495(1.13)  LDD1643  [1]
 LDCM0440  CL5 HEK-293T C495(1.13)  LDD1644  [1]
 LDCM0441  CL50 HEK-293T C495(1.08)  LDD1645  [1]
 LDCM0444  CL53 HEK-293T C495(1.39)  LDD1647  [1]
 LDCM0446  CL55 HEK-293T C495(1.17)  LDD1649  [1]
 LDCM0450  CL59 HEK-293T C495(0.86)  LDD1653  [1]
 LDCM0453  CL61 HEK-293T C495(1.16)  LDD1656  [1]
 LDCM0454  CL62 HEK-293T C495(1.27)  LDD1657  [1]
 LDCM0455  CL63 HEK-293T C495(1.23)  LDD1658  [1]
 LDCM0457  CL65 HEK-293T C495(1.36)  LDD1660  [1]
 LDCM0459  CL67 HEK-293T C495(1.38)  LDD1662  [1]
 LDCM0462  CL7 HEK-293T C495(1.25)  LDD1665  [1]
 LDCM0464  CL71 HEK-293T C495(1.09)  LDD1667  [1]
 LDCM0466  CL73 HEK-293T C495(1.16)  LDD1669  [1]
 LDCM0467  CL74 HEK-293T C495(1.01)  LDD1670  [1]
 LDCM0470  CL77 HEK-293T C495(1.20)  LDD1673  [1]
 LDCM0472  CL79 HEK-293T C495(1.15)  LDD1675  [1]
 LDCM0477  CL83 HEK-293T C495(1.09)  LDD1680  [1]
 LDCM0479  CL85 HEK-293T C495(1.18)  LDD1682  [1]
 LDCM0480  CL86 HEK-293T C495(1.08)  LDD1683  [1]
 LDCM0481  CL87 HEK-293T C495(1.22)  LDD1684  [1]
 LDCM0483  CL89 HEK-293T C495(1.18)  LDD1686  [1]
 LDCM0486  CL91 HEK-293T C495(1.40)  LDD1689  [1]
 LDCM0490  CL95 HEK-293T C495(0.96)  LDD1693  [1]
 LDCM0492  CL97 HEK-293T C495(1.10)  LDD1695  [1]
 LDCM0493  CL98 HEK-293T C495(0.92)  LDD1696  [1]
 LDCM0494  CL99 HEK-293T C495(1.05)  LDD1697  [1]
 LDCM0495  E2913 HEK-293T C495(1.08)  LDD1698  [1]
 LDCM0468  Fragment33 HEK-293T C495(1.20)  LDD1671  [1]
 LDCM0427  Fragment51 HEK-293T C495(1.10)  LDD1631  [1]

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402