Details of the Target

General Information of Target

Target ID LDTP10875
Target Name D-aspartate oxidase (DDO)
Gene Name DDO
Gene ID 8528
Synonyms
D-aspartate oxidase; DASOX; DASPO; DDO; EC 1.4.3.1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGVNQSVGFPPVTGPHLVGCGDVMEGQNLQGSFFRLFYPCQKAEETMEQPLWIPRYEYCT
GLAEYLQFNKRCGGLLFNLAVGSCRLPVSWNGPFKTKDSGYPLIIFSHGLGAFRTLYSAF
CMELASRGFVVAVPEHRDRSAATTYFCKQAPEENQPTNESLQEEWIPFRRVEEGEKEFHV
RNPQVHQRVSECLRVLKILQEVTAGQTVFNILPGGLDLMTLKGNIDMSRVAVMGHSFGGA
TAILALAKETQFRCAVALDAWMFPLERDFYPKARGPVFFINTEKFQTMESVNLMKKICAQ
HEQSRIITVLGSVHRSQTDFAFVTGNLIGKFFSTETRGSLDPYEGQEVMVRAMLAFLQKH
LDLKEDYNQWNNLIEGIGPSLTPGAPHHLSSL
Target Bioclass
Enzyme
Family
DAMOX/DASOX family
Subcellular location
Peroxisome
Function
Selectively catalyzes the oxidative deamination of acidic amino acids. Suppresses the level of D-aspartate in the brain, an amino acid that can act as an agonist for glutamate receptors. Protects the organism from the toxicity of D-amino acids. May also function in the intestine.
Uniprot ID
Q99489
Ensemble ID
ENST00000368923.8
HGNC ID
HGNC:2727
ChEMBL ID
CHEMBL5887

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
MFE319 SNV: p.Y130H .
MOLT4 SNV: p.A48V; p.Y223Ter .
OV90 SNV: p.S35A .
RCC10RGB SNV: p.R164P .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C356(2.51)  LDD3439  [1]
BTD
 Probe Info  		C269(1.93)  LDD1700  [2]
IPM
 Probe Info  		C269(28.63)  LDD1701  [2]
Acrolein
 Probe Info  		C328(0.00); H330(0.00)  LDD0221  [3]
TFBX
 Probe Info  		N.A.  LDD0148  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C269(0.95)  LDD2112  [2]
 LDCM0502  1-(Cyanoacetyl)piperidine MDA-MB-231 C269(0.65)  LDD2095  [2]
 LDCM0537  2-Cyano-N,N-dimethylacetamide MDA-MB-231 C269(0.95)  LDD2130  [2]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C269(1.16)  LDD2117  [2]
 LDCM0539  3-(4-Isopropylpiperazin-1-yl)-3-oxopropanenitrile MDA-MB-231 C269(0.50)  LDD2132  [2]
 LDCM0545  Acetamide MDA-MB-231 C269(0.61)  LDD2138  [2]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C269(7.40)  LDD1702  [2]
 LDCM0107  IAA HeLa C328(0.00); H330(0.00)  LDD0221  [3]
 LDCM0022  KB02 EGI-1 C356(1.22)  LDD2323  [1]
 LDCM0023  KB03 MDA-MB-231 C269(28.63)  LDD1701  [2]
 LDCM0024  KB05 SNU-1196 C356(2.51)  LDD3439  [1]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C269(0.86)  LDD2121  [2]
 LDCM0109  NEM HeLa C328(0.00); H330(0.00)  LDD0224  [3]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C269(0.97)  LDD2099  [2]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C269(0.81)  LDD2104  [2]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C269(0.65)  LDD2106  [2]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C269(0.97)  LDD2109  [2]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C269(1.02)  LDD2114  [2]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C269(0.66)  LDD2120  [2]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C269(0.76)  LDD2125  [2]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C269(1.05)  LDD2127  [2]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C269(1.54)  LDD2136  [2]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C269(1.93)  LDD1700  [2]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C269(0.68)  LDD2140  [2]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C269(2.67)  LDD2144  [2]
 LDCM0559  Nucleophilic fragment 9b MDA-MB-231 C269(2.64)  LDD2153  [2]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
4 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255