Details of the Target

General Information of Target

Target ID LDTP10821
Target Name Cytochrome P450 2S1 (CYP2S1)
Gene Name CYP2S1
Gene ID 29785
Synonyms
Cytochrome P450 2S1; EC 1.14.14.-; CYPIIS1; Hydroperoxy icosatetraenoate dehydratase; EC 4.2.1.152; Thromboxane-A synthase; EC 5.3.99.5
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MKHIPVLEDGPWKTVCVKELNGLKKLKRKGKEPARRANGYKTFRLDLEAPEPRAVATNGL
RDRTHRLQPVPVPVPVPVPVAPAVPPRGGTDTAGERGGSRAPEVSDARKRCFALGAVGPG
LPTPPPPPPPAPQSQAPGGPEAQPFREPGLRPRILLCAPPARPAPSAPPAPPAPPESTVR
PAPPTRPGESSYSSISHVIYNNHQDSSASPRKRPGEATAASSEIKALQQTRRLLANARER
TRVHTISAAFEALRKQVPCYSYGQKLSKLAILRIACNYILSLARLADLDYSADHSNLSFS
ECVQRCTRTLQAEGRAKKRKE
Target Bioclass
Enzyme
Family
Cytochrome P450 family
Subcellular location
Endoplasmic reticulum membrane
Function
A cytochrome P450 monooxygenase involved in the metabolism of retinoids and eicosanoids. In epidermis, may contribute to the oxidative metabolism of all-trans-retinoic acid. For this activity, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Additionally, displays peroxidase and isomerase activities toward various oxygenated eicosanoids such as prostaglandin H2 (PGH2) and hydroperoxyeicosatetraenoates (HPETEs). Independently of cytochrome P450 reductase, NADPH, and O2, catalyzes the breakdown of PGH2 to hydroxyheptadecatrienoic acid (HHT) and malondialdehyde (MDA), which is known to act as a mediator of DNA damage.
Uniprot ID
Q96SQ9
Ensemble ID
ENST00000310054.9
HGNC ID
HGNC:15654
ChEMBL ID
CHEMBL4523986

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
BXPC3 SNV: p.P342Q DBIA    Probe Info 
CAL27 SNV: p.K320Ter DBIA    Probe Info 
HEC1B SNV: p.A95G .
L428 SNV: p.R334W .
OVK18 SNV: p.R59W .
RL952 SNV: p.F430S .
SAOS2 SNV: p.L299F .
TCCSUP SNV: p.P322S .
TOV21G SNV: p.E96Ter .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C440(1.79)  LDD3314  [1]
WYneN
 Probe Info  		N.A.  LDD0021  [2]
IPM
 Probe Info  		N.A.  LDD2156  [3]
AOyne
 Probe Info  		13.20  LDD0443  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C440(0.95)  LDD1507  [5]
 LDCM0259  AC14 HEK-293T C440(0.92)  LDD1512  [5]
 LDCM0276  AC17 HEK-293T C440(0.95)  LDD1515  [5]
 LDCM0281  AC21 HEK-293T C440(1.07)  LDD1520  [5]
 LDCM0282  AC22 HEK-293T C440(0.81)  LDD1521  [5]
 LDCM0285  AC25 HEK-293T C440(1.02)  LDD1524  [5]
 LDCM0289  AC29 HEK-293T C440(0.96)  LDD1528  [5]
 LDCM0291  AC30 HEK-293T C440(1.01)  LDD1530  [5]
 LDCM0294  AC33 HEK-293T C440(0.95)  LDD1533  [5]
 LDCM0298  AC37 HEK-293T C440(0.87)  LDD1537  [5]
 LDCM0299  AC38 HEK-293T C440(1.08)  LDD1538  [5]
 LDCM0303  AC41 HEK-293T C440(1.02)  LDD1542  [5]
 LDCM0307  AC45 HEK-293T C440(1.01)  LDD1546  [5]
 LDCM0308  AC46 HEK-293T C440(1.06)  LDD1547  [5]
 LDCM0311  AC49 HEK-293T C440(0.87)  LDD1550  [5]
 LDCM0312  AC5 HEK-293T C440(1.05)  LDD1551  [5]
 LDCM0316  AC53 HEK-293T C440(0.85)  LDD1555  [5]
 LDCM0317  AC54 HEK-293T C440(1.02)  LDD1556  [5]
 LDCM0320  AC57 HEK-293T C440(0.99)  LDD1559  [5]
 LDCM0323  AC6 HEK-293T C440(1.02)  LDD1562  [5]
 LDCM0325  AC61 HEK-293T C440(1.08)  LDD1564  [5]
 LDCM0326  AC62 HEK-293T C440(1.11)  LDD1565  [5]
 LDCM0248  AKOS034007472 HEK-293T C440(0.96)  LDD1511  [5]
 LDCM0356  AKOS034007680 HEK-293T C440(1.07)  LDD1570  [5]
 LDCM0367  CL1 HEK-293T C440(1.30)  LDD1571  [5]
 LDCM0368  CL10 HEK-293T C440(1.83)  LDD1572  [5]
 LDCM0370  CL101 HEK-293T C440(1.01)  LDD1574  [5]
 LDCM0371  CL102 HEK-293T C440(1.18)  LDD1575  [5]
 LDCM0374  CL105 HEK-293T C440(0.95)  LDD1578  [5]
 LDCM0375  CL106 HEK-293T C440(0.97)  LDD1579  [5]
 LDCM0378  CL109 HEK-293T C440(1.40)  LDD1582  [5]
 LDCM0380  CL110 HEK-293T C440(1.31)  LDD1584  [5]
 LDCM0383  CL113 HEK-293T C440(0.91)  LDD1587  [5]
 LDCM0384  CL114 HEK-293T C440(1.22)  LDD1588  [5]
 LDCM0387  CL117 HEK-293T C440(0.91)  LDD1591  [5]
 LDCM0388  CL118 HEK-293T C440(1.02)  LDD1592  [5]
 LDCM0392  CL121 HEK-293T C440(1.03)  LDD1596  [5]
 LDCM0393  CL122 HEK-293T C440(1.04)  LDD1597  [5]
 LDCM0396  CL125 HEK-293T C440(0.93)  LDD1600  [5]
 LDCM0397  CL126 HEK-293T C440(1.12)  LDD1601  [5]
 LDCM0400  CL13 HEK-293T C440(0.97)  LDD1604  [5]
 LDCM0401  CL14 HEK-293T C440(0.92)  LDD1605  [5]
 LDCM0404  CL17 HEK-293T C440(1.44)  LDD1608  [5]
 LDCM0407  CL2 HEK-293T C440(0.92)  LDD1611  [5]
 LDCM0409  CL21 HEK-293T C440(1.89)  LDD1613  [5]
 LDCM0410  CL22 HEK-293T C440(1.21)  LDD1614  [5]
 LDCM0413  CL25 HEK-293T C440(1.76)  LDD1617  [5]
 LDCM0414  CL26 HEK-293T C440(0.92)  LDD1618  [5]
 LDCM0417  CL29 HEK-293T C440(1.10)  LDD1621  [5]
 LDCM0422  CL33 HEK-293T C440(2.17)  LDD1626  [5]
 LDCM0423  CL34 HEK-293T C440(1.39)  LDD1627  [5]
 LDCM0426  CL37 HEK-293T C440(1.22)  LDD1630  [5]
 LDCM0431  CL41 HEK-293T C440(0.96)  LDD1635  [5]
 LDCM0435  CL45 HEK-293T C440(1.68)  LDD1639  [5]
 LDCM0436  CL46 HEK-293T C440(1.38)  LDD1640  [5]
 LDCM0439  CL49 HEK-293T C440(1.06)  LDD1643  [5]
 LDCM0440  CL5 HEK-293T C440(1.04)  LDD1644  [5]
 LDCM0441  CL50 HEK-293T C440(1.10)  LDD1645  [5]
 LDCM0444  CL53 HEK-293T C440(1.23)  LDD1647  [5]
 LDCM0448  CL57 HEK-293T C440(1.51)  LDD1651  [5]
 LDCM0449  CL58 HEK-293T C440(1.44)  LDD1652  [5]
 LDCM0453  CL61 HEK-293T C440(1.09)  LDD1656  [5]
 LDCM0454  CL62 HEK-293T C440(0.86)  LDD1657  [5]
 LDCM0457  CL65 HEK-293T C440(0.98)  LDD1660  [5]
 LDCM0461  CL69 HEK-293T C440(1.40)  LDD1664  [5]
 LDCM0463  CL70 HEK-293T C440(1.17)  LDD1666  [5]
 LDCM0466  CL73 HEK-293T C440(1.31)  LDD1669  [5]
 LDCM0467  CL74 HEK-293T C440(0.95)  LDD1670  [5]
 LDCM0470  CL77 HEK-293T C440(1.41)  LDD1673  [5]
 LDCM0475  CL81 HEK-293T C440(1.20)  LDD1678  [5]
 LDCM0476  CL82 HEK-293T C440(1.04)  LDD1679  [5]
 LDCM0479  CL85 HEK-293T C440(1.15)  LDD1682  [5]
 LDCM0480  CL86 HEK-293T C440(1.04)  LDD1683  [5]
 LDCM0483  CL89 HEK-293T C440(1.11)  LDD1686  [5]
 LDCM0484  CL9 HEK-293T C440(1.65)  LDD1687  [5]
 LDCM0488  CL93 HEK-293T C440(1.87)  LDD1691  [5]
 LDCM0489  CL94 HEK-293T C440(1.17)  LDD1692  [5]
 LDCM0492  CL97 HEK-293T C440(1.39)  LDD1695  [5]
 LDCM0493  CL98 HEK-293T C440(1.07)  LDD1696  [5]
 LDCM0427  Fragment51 HEK-293T C440(1.00)  LDD1631  [5]
 LDCM0022  KB02 A2780 C440(2.07)  LDD2254  [1]
 LDCM0023  KB03 A2780 C440(6.49)  LDD2671  [1]
 LDCM0024  KB05 IGR37 C440(1.79)  LDD3314  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Keratin-associated protein 10-8 (KRTAP10-8) KRTAP type 10 family P60410

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
3 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
4 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402