Details of the Target

General Information of Target

Target ID LDTP10726
Target Name Kinesin-like protein KIF20B (KIF20B)
Gene Name KIF20B
Gene ID 9585
Synonyms
KRMP1; MPHOSPH1; Kinesin-like protein KIF20B; Cancer/testis antigen 90; CT90; Kinesin family member 20B; Kinesin-related motor interacting with PIN1; M-phase phosphoprotein 1; MPP1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEEKRRRARVQGAWAAPVKSQAIAQPATTAKSHLHQKPGQTWKNKEHHLSDREFVFKEPQ
QVVRRAPEPRVIDREGVYEISLSPTGVSRVCLYPGFVDVKEADWILEQLCQDVPWKQRTG
IREDITYQQPRLTAWYGELPYTYSRITMEPNPHWHPVLRTLKNRIEENTGHTFNSLLCNL
YRNEKDSVDWHSDDEPSLGRCPIIASLSFGATRTFEMRKKPPPEENGDYTYVERVKIPLD
HGTLLIMEGATQADWQHRVPKEYHSREPRVNLTFRTVYPDPRGAPW
Target Type
Literature-reported
Target Bioclass
Enzyme
Family
TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family
Subcellular location
Nucleus
Function
Plus-end-directed motor enzyme that is required for completion of cytokinesis. Required for proper midbody organization and abscission in polarized cortical stem cells. Plays a role in the regulation of neuronal polarization by mediating the transport of specific cargos. Participates in the mobilization of SHTN1 and in the accumulation of PIP3 in the growth cone of primary hippocampal neurons in a tubulin and actin-dependent manner. In the developing telencephalon, cooperates with SHTN1 to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex. Involved in cerebral cortex growth. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression.
TTD ID
T92865
Uniprot ID
Q96Q89
DrugMap ID
TTQECT2
Ensemble ID
ENST00000260753.8
HGNC ID
HGNC:7212
ChEMBL ID
CHEMBL2021752

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL72 Deletion: p.N1328TfsTer7 .
CALU6 SNV: p.Q955K DBIA    Probe Info 
DU145 Deletion: p.V1353WfsTer15 .
FUOV1 Substitution: p.N756V DBIA    Probe Info 
HCT15 SNV: p.V1763A .
HDQP1 SNV: p.E1432Q .
HEC1 SNV: p.E277K .
HEC1B SNV: p.E277K .
HSC3 SNV: p.T1407I .
HT115 SNV: p.R574I .
HUH1 Substitution: p.N756V .
ICC9 Deletion: p.S930RfsTer10 .
JVM3 SNV: p.K1073R .
KASUMI2 Substitution: p.N756V .
MCC13 SNV: p.R1778P .
MDAMB453 SNV: p.D860H .
MDST8 SNV: p.S42F DBIA    Probe Info 
MFE296 Deletion: p.S1075VfsTer18 .
MFE319 Deletion: p.K592RfsTer4 .
MOLT4 SNV: p.L1225P .
NOMO1 SNV: p.T154S .
REC1 SNV: p.E590D DBIA    Probe Info 
REH SNV: p.R328K .
RT112 SNV: p.E225Q .
SHP77 SNV: p.M1467I .
SKHEP1 SNV: p.K296E .
SNU1 Insertion: p.S1075KfsTer5 DBIA    Probe Info 
SW1271 SNV: p.R1677I .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 12 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
Jackson_1
 Probe Info  		20.00  LDD0122  [2]
DBIA
 Probe Info  		C480(3.87); C1210(1.24)  LDD3342  [3]
NAIA_5
 Probe Info  		C1158(1.06)  LDD2227  [4]
CY-1
 Probe Info  		N.A.  LDD0246  [5]
IA-alkyne
 Probe Info  		N.A.  LDD0163  [6]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [7]
ENE
 Probe Info  		N.A.  LDD0006  [8]
IPM
 Probe Info  		N.A.  LDD0005  [8]
NPM
 Probe Info  		N.A.  LDD0016  [8]
VSF
 Probe Info  		N.A.  LDD0007  [8]
AOyne
 Probe Info  		14.20  LDD0443  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0632  CL-Sc Hep-G2 C1158(1.06)  LDD2227  [4]
 LDCM0634  CY-0357 Hep-G2 C1642(50.00)  LDD2228  [4]
 LDCM0022  KB02 HEK-293T C1642(0.98); C96(0.87); C480(0.89)  LDD1492  [10]
 LDCM0023  KB03 HEK-293T C1642(0.97); C96(1.01); C480(1.03)  LDD1497  [10]
 LDCM0024  KB05 NCI-H1155 C480(3.87); C1210(1.24)  LDD3342  [3]

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Appendage and Scaffold Diverse Fully Functionalized Small-Molecule Probes via a Minimalist Terminal Alkyne-Aliphatic Diazirine Isocyanide. J Org Chem. 2018 Sep 21;83(18):11245-11253. doi: 10.1021/acs.joc.8b01831. Epub 2018 Aug 31.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
6 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
7 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
8 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
9 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
10 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402