Details of the Target

General Information of Target

Target ID LDTP10060
Target Name E3 ubiquitin-protein ligase RNF166 (RNF166)
Gene Name RNF166
Gene ID 115992
Synonyms
E3 ubiquitin-protein ligase RNF166; EC 2.3.2.27; RING finger protein 166; RING-type E3 ubiquitin transferase RNF166
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MKAFHTFCVVLLVFGSVSEAKFDDFEDEEDIVEYDDNDFAEFEDVMEDSVTESPQRVIIT
EDDEDETTVELEGQDENQEGDFEDADTQEGDTESEPYDDEEFEGYEDKPDTSSSKNKDPI
TIVDVPAHLQNSWESYYLEILMVTGLLAYIMNYIIGKNKNSRLAQAWFNTHRELLESNFT
LVGDDGTNKEATSTGKLNQENEHIYNLWCSGRVCCEGMLIQLRFLKRQDLLNVLARMMRP
VSDQVQIKVTMNDEDMDTYVFAVGTRKALVRLQKEMQDLSEFCSDKPKSGAKYGLPDSLA
ILSEMGEVTDGMMDTKMVHFLTHYADKIESVHFSDQFSGPKIMQEEGQPLKLPDTKRTLL
FTFNVPGSGNTYPKDMEALLPLMNMVIYSIDKAKKFRLNREGKQKADKNRARVEENFLKL
THVQRQEAAQSRREEKKRAEKERIMNEEDPEKQRRLEEAALRREQKKLEKKQMKMKQIKV
KAM
Target Bioclass
Enzyme
Subcellular location
Cytoplasm
Function
E3 ubiquitin-protein ligase that promotes the ubiquitination of different substrates. In turn, participates in different biological processes including interferon production or autophagy. Plays a role in the activation of RNA virus-induced interferon-beta production by promoting the ubiquitination of TRAF3 and TRAF6. Also plays a role in the early recruitment of autophagy adapters to bacteria. Mediates 'Lys-29' and 'Lys-33'-linked ubiquitination of SQSTM1 leading to xenophagic targeting of bacteria and inhibition of their replication.
Uniprot ID
Q96A37
Ensemble ID
ENST00000312838.9
HGNC ID
HGNC:28856

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C127(0.96)  LDD1513  [1]
IPM
 Probe Info  		N.A.  LDD2156  [2]
TFBX
 Probe Info  		N.A.  LDD0148  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0270  AC15 HEK-293T C127(0.96)  LDD1513  [1]
 LDCM0283  AC23 HEK-293T C127(1.14)  LDD1522  [1]
 LDCM0292  AC31 HEK-293T C127(1.06)  LDD1531  [1]
 LDCM0300  AC39 HEK-293T C127(0.92)  LDD1539  [1]
 LDCM0309  AC47 HEK-293T C127(1.07)  LDD1548  [1]
 LDCM0318  AC55 HEK-293T C127(0.92)  LDD1557  [1]
 LDCM0327  AC63 HEK-293T C127(0.93)  LDD1566  [1]
 LDCM0334  AC7 HEK-293T C127(0.91)  LDD1568  [1]
 LDCM0371  CL102 HEK-293T C127(1.01)  LDD1575  [1]
 LDCM0372  CL103 HEK-293T C127(0.96)  LDD1576  [1]
 LDCM0375  CL106 HEK-293T C127(0.89)  LDD1579  [1]
 LDCM0376  CL107 HEK-293T C127(0.99)  LDD1580  [1]
 LDCM0379  CL11 HEK-293T C127(1.13)  LDD1583  [1]
 LDCM0380  CL110 HEK-293T C127(0.92)  LDD1584  [1]
 LDCM0381  CL111 HEK-293T C127(0.97)  LDD1585  [1]
 LDCM0384  CL114 HEK-293T C127(1.06)  LDD1588  [1]
 LDCM0385  CL115 HEK-293T C127(0.87)  LDD1589  [1]
 LDCM0388  CL118 HEK-293T C127(1.04)  LDD1592  [1]
 LDCM0389  CL119 HEK-293T C127(0.93)  LDD1593  [1]
 LDCM0393  CL122 HEK-293T C127(1.04)  LDD1597  [1]
 LDCM0394  CL123 HEK-293T C127(1.03)  LDD1598  [1]
 LDCM0397  CL126 HEK-293T C127(1.15)  LDD1601  [1]
 LDCM0398  CL127 HEK-293T C127(0.99)  LDD1602  [1]
 LDCM0401  CL14 HEK-293T C127(1.10)  LDD1605  [1]
 LDCM0402  CL15 HEK-293T C127(1.03)  LDD1606  [1]
 LDCM0407  CL2 HEK-293T C127(1.13)  LDD1611  [1]
 LDCM0411  CL23 HEK-293T C127(1.03)  LDD1615  [1]
 LDCM0414  CL26 HEK-293T C127(1.12)  LDD1618  [1]
 LDCM0415  CL27 HEK-293T C127(0.95)  LDD1619  [1]
 LDCM0418  CL3 HEK-293T C127(0.97)  LDD1622  [1]
 LDCM0424  CL35 HEK-293T C127(1.01)  LDD1628  [1]
 LDCM0428  CL39 HEK-293T C127(0.94)  LDD1632  [1]
 LDCM0437  CL47 HEK-293T C127(0.97)  LDD1641  [1]
 LDCM0441  CL50 HEK-293T C127(1.04)  LDD1645  [1]
 LDCM0450  CL59 HEK-293T C127(0.90)  LDD1653  [1]
 LDCM0454  CL62 HEK-293T C127(1.08)  LDD1657  [1]
 LDCM0455  CL63 HEK-293T C127(0.99)  LDD1658  [1]
 LDCM0464  CL71 HEK-293T C127(0.84)  LDD1667  [1]
 LDCM0467  CL74 HEK-293T C127(1.04)  LDD1670  [1]
 LDCM0477  CL83 HEK-293T C127(1.05)  LDD1680  [1]
 LDCM0480  CL86 HEK-293T C127(1.16)  LDD1683  [1]
 LDCM0481  CL87 HEK-293T C127(0.90)  LDD1684  [1]
 LDCM0490  CL95 HEK-293T C127(1.31)  LDD1693  [1]
 LDCM0493  CL98 HEK-293T C127(0.92)  LDD1696  [1]
 LDCM0494  CL99 HEK-293T C127(1.08)  LDD1697  [1]
 LDCM0495  E2913 HEK-293T C127(0.95)  LDD1698  [1]
 LDCM0468  Fragment33 HEK-293T C127(0.98)  LDD1671  [1]
 LDCM0427  Fragment51 HEK-293T C127(1.04)  LDD1631  [1]
 LDCM0022  KB02 COLO-679 C88(1.00)  LDD2305  [4]
 LDCM0023  KB03 COLO-679 C88(1.17)  LDD2722  [4]
 LDCM0024  KB05 COLO-792 C88(0.81)  LDD3140  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ubiquitin-conjugating enzyme E2 D4 (UBE2D4) Ubiquitin-conjugating enzyme family Q9Y2X8
Ubiquitin-conjugating enzyme E2 K (UBE2K) Ubiquitin-conjugating enzyme family P61086
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
TAR DNA-binding protein 43 (TARDBP) . Q13148
XIAP-associated factor 1 (XAF1) . Q6GPH4

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
2 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840