Details of the Target

General Information of Target

Target ID LDTP10042
Target Name Protein TMEPAI (PMEPA1)
Gene Name PMEPA1
Gene ID 56937
Synonyms
STAG1; TMEPAI; Protein TMEPAI; Prostate transmembrane protein androgen induced 1; Solid tumor-associated 1 protein; Transmembrane prostate androgen-induced protein
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MRGQRSLLLGPARLCLRLLLLLGYRRRCPPLLRGLVQRWRYGKVCLRSLLYNSFGGSDTA
VDAAFEPVYWLVDNVIRWFGVVFVVLVIVLTGSIVAIAYLCVLPLILRTYSVPRLCWHFF
YSHWNLILIVFHYYQAITTPPGYPPQGRNDIATVSICKKCIYPKPARTHHCSICNRCVLK
MDHHCPWLNNCVGHYNHRYFFSFCFFMTLGCVYCSYGSWDLFREAYAAIEKMKQLDKNKL
QAVANQTYHQTPPPTFSFRERMTHKSLVYLWFLCSSVALALGALTVWHAVLISRGETSIE
RHINKKERRRLQAKGRVFRNPYNYGCLDNWKVFLGVDTGRHWLTRVLLPSSHLPHGNGMS
WEPPPWVTAHSASVMAV
Target Bioclass
Other
Family
PMEPA1 family
Subcellular location
Early endosome membrane
Function
Functions as a negative regulator of TGF-beta signaling and thereby probably plays a role in cell proliferation, differentiation, apoptosis, motility, extracellular matrix production and immunosuppression. In the canonical TGF-beta pathway, ZFYVE9/SARA recruits the intracellular signal transducer and transcriptional modulators SMAD2 and SMAD3 to the TGF-beta receptor. Phosphorylated by the receptor, SMAD2 and SMAD3 then form a heteromeric complex with SMAD4 that translocates to the nucleus to regulate transcription. Through interaction with SMAD2 and SMAD3, LDLRAD4 may compete with ZFYVE9 and SMAD4 and prevent propagation of the intracellular signal. Also involved in down-regulation of the androgen receptor (AR), enhancing ubiquitination and proteasome-mediated degradation of AR, probably by recruiting NEDD4.
Uniprot ID
Q969W9
Ensemble ID
ENST00000265626.8
HGNC ID
HGNC:14107

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HKGZCC SNV: p.P158T .
JURKAT Deletion: p.S209AfsTer61 .
MCAS SNV: p.P94T .
ONS76 SNV: p.P105L .
RL952 SNV: p.G101C .
SNGM Insertion: p.I266TfsTer5 .
SW403 SNV: p.P123T .
TOV21G SNV: p.R81S .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C206(0.95); C218(0.95)  LDD0078  [1]
W1
 Probe Info  		Q126(0.62); R127(0.65)  LDD0239  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0020  ARS-1620 HCC44 C206(0.95); C218(0.95)  LDD0078  [1]
 LDCM0112  W16 Hep-G2 Q126(0.62); R127(0.65)  LDD0239  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ubiquitin thioesterase OTUB2 (OTUB2) Peptidase C65 family Q96DC9
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Small glutamine-rich tetratricopeptide repeat-containing protein alpha (SGTA) SGT family O43765
Ubiquilin-2 (UBQLN2) . Q9UHD9

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.