Details of the Target

General Information of Target

Target ID LDTP09703
Target Name Junctophilin-3 (JPH3)
Gene Name JPH3
Gene ID 57338
Synonyms
JP3; TNRC22; Junctophilin-3; JP-3; Junctophilin type 3; Trinucleotide repeat-containing gene 22 protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSSGGRFNFDDGGSYCGGWEDGKAHGHGVCTGPKGQGEYTGSWSHGFEVLGVYTWPSGNT
YQGTWAQGKRHGIGLESKGKWVYKGEWTHGFKGRYGVRECAGNGAKYEGTWSNGLQDGYG
TETYSDGGTYQGQWVGGMRQGYGVRQSVPYGMAAVIRSPLRTSINSLRSEHTNGTALHPD
ASPAVAGSPAVSRGGFVLVAHSDSEILKSKKKGLFRRSLLSGLKLRKSESKSSLASQRSK
QSSFRSEAGMSTVSSTASDIHSTISLGEAEAELAVIEDDIDATTTETYVGEWKNDKRSGF
GVSQRSDGLKYEGEWASNRRHGYGCMTFPDGTKEEGKYKQNILVGGKRKNLIPLRASKIR
EKVDRAVEAAERAATIAKQKAEIAASRTSHSRAKAEAALTAAQKAQEEARIARITAKEFS
PSFQHRENGLEYQRPKRQTSCDDIEVLSTGTPLQQESPELYRKGTTPSDLTPDDSPLQSF
PTSPAATPPPAPAARNKVAHFSRQVSVDEERGGDIQMLLEGRAGDCARSSWGEEQAGGSR
GVRSGALRGGLLVDDFRTRGSGRKQPGNPKPRERRTESPPVFTWTSHHRASNHSPGGSRL
LELQEEKLSNYRMEMKPLLRMETHPQKRRYSKGGACRGLGDDHRPEDRGFGVQRLRSKAQ
NKENFRPASSAEPAVQKLASLRLGGAEPRLLRWDLTFSPPQKSLPVALESDEENGDELKS
STGSAPILVVMVILLNIGVAILFINFFI
Target Bioclass
Transporter and channel
Family
Junctophilin family
Subcellular location
Cell membrane
Function
Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels. JPH3 is brain-specific and appears to have an active role in certain neurons involved in motor coordination and memory.
Uniprot ID
Q8WXH2
Ensemble ID
ENST00000284262.3
HGNC ID
HGNC:14203

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO678 Substitution: p.S721K .
HT115 SNV: p.S228L .
HT29 SNV: p.G308E .
IM95 Deletion: p.L354CfsTer117 .
Ishikawa (Heraklio) 02 ER Deletion: p.L354CfsTer117 .
JURKAT SNV: p.T60M .
MCC26 SNV: p.D307N .
MFE319 Deletion: p.V581CfsTer28
SNV: p.S239N		 .
OVCAR5 SNV: p.G46S .
SNU1 SNV: p.D473N .
SW948 SNV: p.C636Y .
TE1 SNV: p.D641E .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		N.A.  LDD0241  [1]
DBIA
 Probe Info  		C30(1.04)  LDD3331  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C30(1.06)  LDD1508  [3]
 LDCM0277  AC18 HEK-293T C30(1.03)  LDD1516  [3]
 LDCM0279  AC2 HEK-293T C30(1.02)  LDD1518  [3]
 LDCM0286  AC26 HEK-293T C30(0.95)  LDD1525  [3]
 LDCM0295  AC34 HEK-293T C30(1.06)  LDD1534  [3]
 LDCM0304  AC42 HEK-293T C30(1.07)  LDD1543  [3]
 LDCM0313  AC50 HEK-293T C30(1.12)  LDD1552  [3]
 LDCM0321  AC58 HEK-293T C30(1.08)  LDD1560  [3]
 LDCM0369  CL100 HEK-293T C30(0.88)  LDD1573  [3]
 LDCM0371  CL102 HEK-293T C30(0.99)  LDD1575  [3]
 LDCM0373  CL104 HEK-293T C30(0.95)  LDD1577  [3]
 LDCM0375  CL106 HEK-293T C30(0.92)  LDD1579  [3]
 LDCM0377  CL108 HEK-293T C30(0.94)  LDD1581  [3]
 LDCM0380  CL110 HEK-293T C30(0.88)  LDD1584  [3]
 LDCM0382  CL112 HEK-293T C30(0.90)  LDD1586  [3]
 LDCM0384  CL114 HEK-293T C30(0.78)  LDD1588  [3]
 LDCM0386  CL116 HEK-293T C30(0.97)  LDD1590  [3]
 LDCM0388  CL118 HEK-293T C30(1.00)  LDD1592  [3]
 LDCM0391  CL120 HEK-293T C30(0.92)  LDD1595  [3]
 LDCM0393  CL122 HEK-293T C30(0.95)  LDD1597  [3]
 LDCM0395  CL124 HEK-293T C30(0.95)  LDD1599  [3]
 LDCM0397  CL126 HEK-293T C30(1.02)  LDD1601  [3]
 LDCM0399  CL128 HEK-293T C30(1.00)  LDD1603  [3]
 LDCM0401  CL14 HEK-293T C30(0.86)  LDD1605  [3]
 LDCM0403  CL16 HEK-293T C30(0.76)  LDD1607  [3]
 LDCM0405  CL18 HEK-293T C30(0.97)  LDD1609  [3]
 LDCM0407  CL2 HEK-293T C30(0.97)  LDD1611  [3]
 LDCM0414  CL26 HEK-293T C30(0.95)  LDD1618  [3]
 LDCM0416  CL28 HEK-293T C30(0.94)  LDD1620  [3]
 LDCM0419  CL30 HEK-293T C30(1.08)  LDD1623  [3]
 LDCM0429  CL4 HEK-293T C30(0.91)  LDD1633  [3]
 LDCM0430  CL40 HEK-293T C30(1.05)  LDD1634  [3]
 LDCM0432  CL42 HEK-293T C30(1.17)  LDD1636  [3]
 LDCM0441  CL50 HEK-293T C30(1.00)  LDD1645  [3]
 LDCM0443  CL52 HEK-293T C30(0.91)  LDD1646  [3]
 LDCM0445  CL54 HEK-293T C30(0.94)  LDD1648  [3]
 LDCM0451  CL6 HEK-293T C30(0.90)  LDD1654  [3]
 LDCM0454  CL62 HEK-293T C30(0.93)  LDD1657  [3]
 LDCM0456  CL64 HEK-293T C30(0.84)  LDD1659  [3]
 LDCM0458  CL66 HEK-293T C30(1.00)  LDD1661  [3]
 LDCM0467  CL74 HEK-293T C30(1.00)  LDD1670  [3]
 LDCM0469  CL76 HEK-293T C30(0.87)  LDD1672  [3]
 LDCM0471  CL78 HEK-293T C30(1.02)  LDD1674  [3]
 LDCM0480  CL86 HEK-293T C30(0.95)  LDD1683  [3]
 LDCM0482  CL88 HEK-293T C30(0.96)  LDD1685  [3]
 LDCM0485  CL90 HEK-293T C30(1.02)  LDD1688  [3]
 LDCM0493  CL98 HEK-293T C30(0.99)  LDD1696  [3]
 LDCM0427  Fragment51 HEK-293T C30(1.00)  LDD1631  [3]
 LDCM0022  KB02 MKN-1 C30(1.39)  LDD2497  [2]
 LDCM0023  KB03 MKN-1 C30(1.22)  LDD2914  [2]
 LDCM0024  KB05 MKN-1 C30(1.04)  LDD3331  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 40 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
GTP:AMP phosphotransferase AK3, mitochondrial (AK3) Adenylate kinase family Q9UIJ7
Aldehyde dehydrogenase 1A1 (ALDH1A1) Aldehyde dehydrogenase family P00352
Cell division cycle protein 23 homolog (CDC23) APC8/CDC23 family Q9UJX2
Piwi-like protein 1 (PIWIL1) Argonaute family Q96J94
Creatine kinase U-type, mitochondrial (CKMT1A; CKMT1B) ATP:guanido phosphotransferase family P12532
NADH dehydrogenase iron-sulfur protein 7, mitochondrial (NDUFS7) Complex I 20 kDa subunit family O75251
Cytochrome P450 26B1 (CYP26B1) Cytochrome P450 family Q9NR63
Eukaryotic translation initiation factor 3 subunit F (EIF3F) EIF-3 subunit F family O00303
FAST kinase domain-containing protein 1, mitochondrial (FASTKD1) FAST kinase family Q53R41
Exostosin-like 2 (EXTL2) Glycosyltransferase 47 family Q9UBQ6
Pyridoxal phosphate phosphatase PHOSPHO2 (PHOSPHO2) PHOSPHO family Q8TCD6
Baculoviral IAP repeat-containing protein 2 (BIRC2) IAP family Q13490
Phosphatidate phosphatase LPIN1 (LPIN1) Lipin family Q14693
Protein-L-isoaspartate O-methyltransferase domain-containing protein 2 (PCMTD2) L-isoaspartyl/D-aspartyl protein methyltransferase family Q9NV79
[F-actin]-monooxygenase MICAL2 (MICAL2) Mical family O94851
Methionine-R-sulfoxide reductase B2, mitochondrial (MSRB2) MsrB Met sulfoxide reductase family Q9Y3D2
Prostaglandin reductase 1 (PTGR1) NADP-dependent oxidoreductase L4BD family Q14914
Nucleoside diphosphate kinase homolog 5 (NME5) NDK family P56597
Ubiquitin carboxyl-terminal hydrolase 10 (USP10) Peptidase C19 family Q14694
Ubiquitin thioesterase OTUB1 (OTUB1) Peptidase C65 family Q96FW1
MPN domain-containing protein (MPND) Peptidase M67 family Q8N594
STAM-binding protein (STAMBP) Peptidase M67C family O95630
ATP-dependent Clp protease proteolytic subunit, mitochondrial (CLPP) Peptidase S14 family Q16740
Proprotein convertase subtilisin/kexin type 7 (PCSK7) Peptidase S8 family Q16549
RNA-binding protein PNO1 (PNO1) PNO1 family Q9NRX1
Serine/threonine-protein kinase 3 (STK3) STE Ser/Thr protein kinase family Q13188
Dual specificity protein phosphatase 18 (DUSP18) Protein-tyrosine phosphatase family Q8NEJ0
Ras-related protein Rab-35 (RAB35) Rab family Q15286
Ras-related protein Rab-38 (RAB38) Rab family P57729
Thioredoxin (TXN) Thioredoxin family P10599
Atlastin-1 (ATL1) GB1/RHD3 GTPase family Q8WXF7
Developmentally-regulated GTP-binding protein 2 (DRG2) OBG GTPase family P55039
E3 ubiquitin-protein ligase TRAIP (TRAIP) TRAIP family Q9BWF2
E3 ubiquitin-protein ligase LNX (LNX1) . Q8TBB1
E3 ubiquitin-protein ligase RNF138 (RNF138) . Q8WVD3
Germ cell-less protein-like 2 (GMCL2) . Q8NEA9
LON peptidase N-terminal domain and RING finger protein 2 (LONRF2) . Q1L5Z9
NEDD4-like E3 ubiquitin-protein ligase WWP2 (WWP2) . O00308
Probable E3 ubiquitin-protein ligase MARCHF10 (MARCHF10) . Q8NA82
Rho-related BTB domain-containing protein 3 (RHOBTB3) . O94955
Transporter and channel
Click To Hide/Show 12 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
V-type proton ATPase subunit B, brain isoform (ATP6V1B2) ATPase alpha/beta chains family P21281
Bcl-2 homologous antagonist/killer (BAK1) Bcl-2 family Q16611
Gap junction alpha-5 protein (GJA5) Connexin family P36382
Choline transporter-like protein 5 (SLC44A5) CTL (choline transporter-like) family Q8NCS7
Transmembrane emp24 domain-containing protein 10 (TMED10) EMP24/GP25L family P49755
Peroxisome proliferator-activated receptor alpha (PPARA) Nuclear hormone receptor family Q07869
Peripheral myelin protein 22 (PMP22) PMP-22/EMP/MP20 family Q01453
Transmembrane channel-like protein 6 (TMC6) TMC family Q7Z403
p53 apoptosis effector related to PMP-22 (PERP) TMEM47 family Q96FX8
Protein YIPF1 (YIPF1) YIP1 family Q9Y548
Membrane protein MLC1 (MLC1) . Q15049
Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1) . Q9BVT8
Transcription factor
Click To Hide/Show 16 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
DNA damage-inducible transcript 3 protein (DDIT3) BZIP family P35638
Mothers against decapentaplegic homolog 3 (SMAD3) Dwarfin/SMAD family P84022
Heat shock transcription factor, Y-linked (HSFY1; HSFY2) HSF family Q96LI6
Myoneurin (MYNN) Krueppel C2H2-type zinc-finger protein family Q9NPC7
Zinc finger and SCAN domain-containing protein 9 (ZSCAN9) Krueppel C2H2-type zinc-finger protein family O15535
Zinc finger protein 19 (ZNF19) Krueppel C2H2-type zinc-finger protein family P17023
Zinc finger protein 20 (ZNF20) Krueppel C2H2-type zinc-finger protein family P17024
Zinc finger protein 232 (ZNF232) Krueppel C2H2-type zinc-finger protein family Q9UNY5
Zinc finger protein 436 (ZNF436) Krueppel C2H2-type zinc-finger protein family Q9C0F3
Zinc finger protein 500 (ZNF500) Krueppel C2H2-type zinc-finger protein family O60304
Zinc finger protein 774 (ZNF774) Krueppel C2H2-type zinc-finger protein family Q6NX45
Zinc finger protein with KRAB and SCAN domains 3 (ZKSCAN3) Krueppel C2H2-type zinc-finger protein family Q9BRR0
Visual system homeobox 2 (VSX2) Paired homeobox family P58304
Homeobox protein OTX1 (OTX1) Paired homeobox family P32242
Zinc finger and BTB domain-containing protein 25 (ZBTB25) . P24278
Zinc finger protein 581 (ZNF581) . Q9P0T4
Immunoglobulin
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Myosin-binding protein H-like (MYBPHL) MyBP family A2RUH7
T-cell surface glycoprotein CD1c (CD1C) . P29017
Cytokine and receptor
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cytokine receptor-like factor 3 (CRLF3) Cytokine receptor-like factor 3 family Q8IUI8
C-C motif chemokine 21 (CCL21) Intercrine beta (chemokine CC) family O00585
Interleukin-22 receptor subunit alpha-1 (IL22RA1) Type II cytokine receptor family Q8N6P7
Other
Click To Hide/Show 69 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ankyrin repeat and SOCS box protein 9 (ASB9) Ankyrin SOCS box (ASB) family Q96DX5
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 (TNFAIP1) BACURD family Q13829
Catenin beta-1 (CTNNB1) Beta-catenin family P35222
Protein BRICK1 (BRK1) BRK1 family Q8WUW1
Electron transfer flavoprotein regulatory factor 1 (ETFRF1) Complex I LYR family Q6IPR1
Protein LZIC (LZIC) CTNNBIP1 family Q8WZA0
CWF19-like protein 2 (CWF19L2) CWF19 family Q2TBE0
Segment polarity protein dishevelled homolog DVL-3 (DVL3) DSH family Q92997
26S proteasome complex subunit SEM1 (SEM1) DSS1/SEM1 family P60896
Splicing factor ESS-2 homolog (ESS2) ESS2 family Q96DF8
Transcobalamin-1 (TCN1) Eukaryotic cobalamin transport proteins family P20061
Centromere protein V (CENPV) Gfa family Q7Z7K6
Histone H3.2 (H3C15; H3C14; H3C13) Histone H3 family Q71DI3
Desmin (DES) Intermediate filament family P17661
Glial fibrillary acidic protein (GFAP) Intermediate filament family P14136
Keratin, type I cytoskeletal 19 (KRT19) Intermediate filament family P08727
Keratin-associated protein 10-8 (KRTAP10-8) KRTAP type 10 family P60410
Protein limb expression 1 homolog (LIX1) LIX1 family Q8N485
Large ribosomal subunit protein mL49 (MRPL49) Mitochondrion-specific ribosomal protein mL49 family Q13405
Nucleolar protein 6 (NOL6) NRAP family Q9H6R4
Nucleosome assembly protein 1-like 3 (NAP1L3) Nucleosome assembly protein (NAP) family Q99457
Protein CIMAP1D (CIMAP1D) ODF3 family Q3SX64
Proteasome activator complex subunit 1 (PSME1) PA28 family Q06323
Polyadenylate-binding protein 3 (PABPC3) Polyadenylate-binding protein type-1 family Q9H361
Secreted frizzled-related protein 4 (SFRP4) Secreted frizzled-related protein (sFRP) family Q6FHJ7
Splicing factor 3A subunit 3 (SF3A3) SF3A3 family Q12874
Pre-mRNA-splicing factor SLU7 (SLU7) SLU7 family O95391
Biglycan (BGN) Small leucine-rich proteoglycan (SLRP) family P21810
Nyctalopin (NYX) Small leucine-rich proteoglycan (SLRP) family Q9GZU5
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 (SMARCD1) SMARCD family Q96GM5
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 (SMARCB1) SNF5 family Q12824
SNW domain-containing protein 1 (SNW1) SNW family Q13573
Spermatogenesis-associated protein 2-like protein (SPATA2L) SPATA2 family Q8IUW3
Pre-mRNA-splicing factor SPF27 (BCAS2) SPF27 family O75934
Serine/arginine-rich splicing factor 4 (SRSF4) Splicing factor SR family Q08170
Tachykinin-3 (TAC3) Tachykinin family Q9UHF0
Protein TASOR 2 (TASOR2) TASOR family Q5VWN6
Transmembrane protein 141 (TMEM141) TMEM141 family Q96I45
Transmembrane protein 186 (TMEM186) TMEM186 family Q96B77
Tetratricopeptide repeat protein 19, mitochondrial (TTC19) TTC19 family Q6DKK2
Small ribosomal subunit protein uS7 (RPS5) Universal ribosomal protein uS7 family P46782
U3 small nucleolar RNA-associated protein 14 homolog A (UTP14A) UTP14 family Q9BVJ6
Vacuolar protein sorting-associated protein 37A (VPS37A) VPS37 family Q8NEZ2
WD repeat domain-containing protein 83 (WDR83) WD repeat MORG1 family Q9BRX9
Epidermal growth factor-like protein 8 (EGFL8) . Q99944
F-box only protein 15 (FBXO15) . Q8NCQ5
F-box/WD repeat-containing protein 1A (BTRC) . Q9Y297
Fanconi anemia group G protein (FANCG) . O15287
Galectin-9C (LGALS9C) . Q6DKI2
Heterogeneous nuclear ribonucleoprotein K (HNRNPK) . P61978
IQ motif and ubiquitin-like domain-containing protein (IQUB) . Q8NA54
Leukocyte receptor cluster member 8 (LENG8) . Q96PV6
Melanoma-associated antigen B3 (MAGEB3) . O15480
mRNA decay activator protein ZFP36 (ZFP36) . P26651
Myosin regulatory light chain 11 (MYL11) . Q96A32
PDZ and LIM domain protein 5 (PDLIM5) . Q96HC4
Period circadian protein homolog 1 (PER1) . O15534
Protein phosphatase 1 regulatory subunit 16A (PPP1R16A) . Q96I34
Ras association domain-containing protein 2 (RASSF2) . P50749
Spermatogenesis-associated protein 22 (SPATA22) . Q8NHS9
Splicing regulator RBM11 (RBM11) . P57052
Sprouty-related, EVH1 domain-containing protein 1 (SPRED1) . Q7Z699
Superkiller complex protein 8 (SKIC8) . Q9GZS3
Suppressor of cytokine signaling 4 (SOCS4) . Q8WXH5
Telethonin (TCAP) . O15273
Ubiquilin-like protein (UBQLNL) . Q8IYU4
Uncharacterized protein C20orf141 (C20orf141) . Q9NUB4
Uncharacterized protein C6orf141 (C6orf141) . Q5SZD1
Uncharacterized protein KIAA0408 (KIAA0408) . Q6ZU52

References

1 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402