Details of the Target

General Information of Target

Target ID LDTP09633
Target Name PEST proteolytic signal-containing nuclear protein (PCNP)
Gene Name PCNP
Gene ID 57092
Synonyms
PEST proteolytic signal-containing nuclear protein; PCNP; PEST-containing nuclear protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MADGKAGDEKPEKSQRAGAAGGPEEEAEKPVKTKTVSSSNGGESSSRSAEKRSAEEEAAD
LPTKPTKISKFGFAIGSQTTKKASAISIKLGSSKPKETVPTLAPKTLSVAAAFNEDEDSE
PEEMPPEAKMRMKNIGRDTPTSAGPNSFNKGKHGFSDNQKLWERNIKSHLGNVHDQDN
Target Bioclass
Other
Subcellular location
Nucleus
Function May be involved in cell cycle regulation.
Uniprot ID
Q8WW12
Ensemble ID
ENST00000265260.8
HGNC ID
HGNC:30023

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
KMS12BM SNV: p.D138V .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
AZ-9
 Probe Info  		10.00  LDD0393  [1]
5E-2FA
 Probe Info  		N.A.  LDD2235  [2]
ATP probe
 Probe Info  		K167(0.00); K150(0.00); K152(0.00); K160(0.00)  LDD0199  [3]
m-APA
 Probe Info  		N.A.  LDD2231  [2]
ATP probe
 Probe Info  		N.A.  LDD0035  [4]
NHS
 Probe Info  		K152(0.00); K67(0.00); K150(0.00); K70(0.00)  LDD0010  [5]
STPyne
 Probe Info  		K150(0.00); K67(0.00)  LDD0009  [5]
1c-yne
 Probe Info  		N.A.  LDD0228  [6]
Acrolein
 Probe Info  		H169(0.00); H174(0.00)  LDD0217  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa H169(0.00); H153(0.00); H174(0.00)  LDD0222  [7]
 LDCM0107  IAA HeLa H169(0.00); H174(0.00)  LDD0221  [7]
 LDCM0109  NEM HeLa H169(0.00); H153(0.00); H174(0.00)  LDD0223  [7]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Kallikrein-6 (KLK6) Peptidase S1 family Q92876
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Telomeric repeat-binding factor 2-interacting protein 1 (TERF2IP) RAP1 family Q9NYB0

References

1 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
2 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
3 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
4 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
5 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
6 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
7 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.