Details of the Target

General Information of Target

Target ID LDTP09481
Target Name Epidermal growth factor receptor kinase substrate 8-like protein 1 (EPS8L1)
Gene Name EPS8L1
Gene ID 54869
Synonyms
DRC3; EPS8R1; Epidermal growth factor receptor kinase substrate 8-like protein 1; EPS8-like protein 1; Epidermal growth factor receptor pathway substrate 8-related protein 1; EPS8-related protein 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSTATGPEAAPKPSAKSIYEQRKRYSTVVMADVSQYPVNHLVTFCLGEDDGVHTVEDASR
KLAVMDSQGRVWAQEMLLRVSPDHVTLLDPASKEELESYPLGAIVRCDAVMPPGRSRSLL
LLVCQEPERAQPDVHFFQGLRLGAELIREDIQGALHNYRSGRGERRAAALRATQEELQRD
RSPAAETPPLQRRPSVRAVISTVERGAGRGRPQAKPIPEAEEAQRPEPVGTSSNADSASP
DLGPRGPDLAVLQAEREVDILNHVFDDVESFVSRLQKSAEAARVLEHRERGRRSRRRAAG
EGLLTLRAKPPSEAEYTDVLQKIKYAFSLLARLRGNIADPSSPELLHFLFGPLQMIVNTS
GGPEFASSVRRPHLTSDAVALLRDNVTPRENELWTSLGDSWTRPGLELSPEEGPPYRPEF
FSGWEPPVTDPQSRAWEDPVEKQLQHERRRRQQSAPQVAVNGHRDLEPESEPQLESETAG
KWVLCNYDFQARNSSELSVKQRDVLEVLDDSRKWWKVRDPAGQEGYVPYNILTPYPGPRL
HHSQSPARSLNSTPPPPPAPAPAPPPALARPRWDRPRWDSCDSLNGLDPSEKEKFSQMLI
VNEELQARLAQGRSGPSRAVPGPRAPEPQLSPGSDASEVRAWLQAKGFSSGTVDALGVLT
GAQLFSLQKEELRAVSPEEGARVYSQVTVQRSLLEDKEKVSELEAVMEKQKKKVEGEVEM
EVI
Target Bioclass
Other
Family
EPS8 family
Subcellular location
Cytoplasm
Function Stimulates guanine exchange activity of SOS1. May play a role in membrane ruffling and remodeling of the actin cytoskeleton.
Uniprot ID
Q8TE68
Ensemble ID
ENST00000201647.11
HGNC ID
HGNC:21295

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.P311S; p.R618H .
HEC1 SNV: p.R290C; p.E411D .
HEC1B SNV: p.E411D .
HT SNV: p.Y99C; p.A705T .
JURKAT Insertion: p.N602KfsTer48 .
MEWO SNV: .
Substitution: p.E176K		 .
NCIH1048 Deletion: p.K709SfsTer25
SNV: p.K709Q		 .
OCIAML2 SNV: p.P456T .
SKNSH SNV: p.P240Q .
SNGM SNV: p.P427L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C-Sul
 Probe Info  		3.96  LDD0066  [1]
DBIA
 Probe Info  		C124(4.48)  LDD3364  [2]
NAIA_5
 Probe Info  		C581(0.98); C581(0.16)  LDD2227  [3]
ATP probe
 Probe Info  		K709(0.00); K711(0.00)  LDD0199  [4]
IA-alkyne
 Probe Info  		C581(0.00); C124(0.00)  LDD0162  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0632  CL-Sc Hep-G2 C581(0.98); C581(0.16)  LDD2227  [3]
 LDCM0634  CY-0357 Hep-G2 C581(0.38)  LDD2228  [3]
 LDCM0022  KB02 AGS C124(2.08)  LDD2263  [2]
 LDCM0023  KB03 AGS C124(2.96)  LDD2680  [2]
 LDCM0024  KB05 NCI-N87 C124(4.48)  LDD3364  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Peroxisomal ATPase PEX1 (PEX1) AAA ATPase family O43933
Disintegrin and metalloproteinase domain-containing protein 19 (ADAM19) . Q9H013
Transporter and channel
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Huntingtin (HTT) Huntingtin family P42858
T-cell surface glycoprotein CD3 epsilon chain (CD3E) . P07766
Wolframin (WFS1) . O76024
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Telomeric repeat-binding factor 2-interacting protein 1 (TERF2IP) RAP1 family Q9NYB0
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cysteine-rich tail protein 1 (CYSRT1) CYSRT1 family A8MQ03
Progranulin (GRN) Granulin family P28799

References

1 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060