Details of the Target

General Information of Target

Target ID LDTP09369
Target Name E3 ubiquitin-protein ligase RNF217 (RNF217)
Gene Name RNF217
Gene ID 154214
Synonyms
C6orf172; IBRDC1; OSTL; E3 ubiquitin-protein ligase RNF217; EC 2.3.2.31; IBR domain-containing protein 1; Opposite STL; RING finger protein 217
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGEEQSTVSGGGGPQESQTLASGTAGHPEPPRPQGDSARAPPLRAASAEPSGGGCGSDWG
CADTSAPEPARSLGPPGWSKSRAPAQPAGLALTGPLNPQTLPLQLELEEEEEEAGDRKEG
GDEQQEAPPGEELEPRTRVGAADGLVLDVLGQRRPSLAKRQVFCSVYCVESDLPEAPASE
QLSPPASPPGAPPVLNPPSTRSSFPSPRLSLPTDSLSPDGGSIELEFYLAPEPFSMPSLL
GAPPYSGLGGVGDPYVPLMVLMCRVCLEDKPIKPLPCCKKAVCEECLKVYLSAQVQLGQV
EIKCPITECFEFLEETTVVYNLTHEDSIKYKYFLELGRIDSSTKPCPQCKHFTTFKKKGH
IPTPSRSESKYKIQCPTCQFVWCFKCHSPWHEGVNCKEYKKGDKLLRHWASEIEHGQRNA
QKCPKCKIHIQRTEGCDHMTCSQCNTNFCYRCGERYRQLRFFGDHTSNLSIFGCKYRYLP
ERPHLRRLVRGSVCAGKLFIAPLIMVLGLALGAIAVVIGLFVFPIYCLCKKQRKRSRTGM
HW
Target Bioclass
Enzyme
Family
RBR family, RNF217 subfamily
Subcellular location
Membrane
Function
E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates the degradation of the iron exporter ferroportin/SLC40A1 and thus regulates iron homeostasis.
Uniprot ID
Q8TC41
Ensemble ID
ENST00000359704.2
HGNC ID
HGNC:21487

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CORL23 SNV: p.P42T .
HS936T Substitution: p.P129F .
KNS42 SNV: p.S411R .
L428 SNV: p.K530R .
MFE319 SNV: p.E325D .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C474(1.04)  LDD1573  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0369  CL100 HEK-293T C474(1.04)  LDD1573  [1]
 LDCM0373  CL104 HEK-293T C474(1.15)  LDD1577  [1]
 LDCM0377  CL108 HEK-293T C474(1.23)  LDD1581  [1]
 LDCM0382  CL112 HEK-293T C474(1.14)  LDD1586  [1]
 LDCM0386  CL116 HEK-293T C474(1.17)  LDD1590  [1]
 LDCM0391  CL120 HEK-293T C474(1.15)  LDD1595  [1]
 LDCM0395  CL124 HEK-293T C474(1.16)  LDD1599  [1]
 LDCM0399  CL128 HEK-293T C474(0.97)  LDD1603  [1]
 LDCM0403  CL16 HEK-293T C474(1.07)  LDD1607  [1]
 LDCM0416  CL28 HEK-293T C474(1.36)  LDD1620  [1]
 LDCM0429  CL4 HEK-293T C474(1.12)  LDD1633  [1]
 LDCM0430  CL40 HEK-293T C474(1.20)  LDD1634  [1]
 LDCM0443  CL52 HEK-293T C474(1.02)  LDD1646  [1]
 LDCM0456  CL64 HEK-293T C474(1.34)  LDD1659  [1]
 LDCM0469  CL76 HEK-293T C474(0.95)  LDD1672  [1]
 LDCM0482  CL88 HEK-293T C474(1.13)  LDD1685  [1]

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402