Details of the Target

General Information of Target

Target ID LDTP09219
Target Name Protein DBF4 homolog B (DBF4B)
Gene Name DBF4B
Gene ID 80174
Synonyms
ASKL1; DRF1; Protein DBF4 homolog B; Activator of S phase kinase-like protein 1; ASK-like protein 1; Chiffon homolog B; Dbf4-related factor 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSEPGKGDDCLELESSMAESRLRAPDLGVSRCLGKCQKNSPGARKHPFSGKSFYLDLPAG
KNLQFLTGAIQQLGGVIEGFLSKEVSYIVSSRREVKAESSGKSHRGCPSPSPSEVRVETS
AMVDPKGSHPRPSRKPVDSVPLSRGKELLQKAIRNQGSISGGGSGGSSSLLTNARSWGVR
ILHVDEMMMHVQQLSLASLCVKKQQPKKPEGTCPAAESRTRKVARLKAPFLKIEDESRKF
RPFHHQFKSFPEISFLGPKDASPFEAPTTLGSMHHTRESKDGEPSPRSAAHTMPRRKKGY
CECCQEAFEELHVHLQSAQHRSFALEAHLYAEVDRIIAQLSHSFADIPFQAGLPRWSGSP
ASDCDPLCPETLHPHQPSHPRAASPRIRKEDSCQASVTQGRAAGQQRWTESLDGVMGPPA
SHTCVSATTLLPALPKGSREQGCLCPCPASFTQSHLVTSLALLPGEWSPAEDMPLHPSQE
NSFAPADIPVKGPLLFPEARPWLMSARCWVRPFPFVTWGCLIPHDTTPLHEEVSPCPCLR
LGYLYLLLTQSLWCRVRVPSLSTAGPIPRTSHPCTLAFPSYLNDHDLGHLCQAKPQGWNT
PQPFLHCGFLAVDSG
Target Bioclass
Other
Subcellular location
Nucleus
Function
Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle.
Uniprot ID
Q8NFT6
Ensemble ID
ENST00000315005.8
HGNC ID
HGNC:17883

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
ICC10 SNV: p.S30Y .
ICC106 SNV: p.S30Y .
ICC108 SNV: p.S30Y .
IGROV1 SNV: p.K6N .
MCC13 SNV: p.P579L .
SW756 SNV: p.E14Ter .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C213(4.06)  LDD2236  [1]
TPP-AC
 Probe Info  		N.A.  LDD0427  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
GTP-binding protein GEM (GEM) RGK family P55040
Zinc finger protein RFP (TRIM27) TRIM/RBCC family P14373
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
MyoD family inhibitor (MDFI) MDFI family Q99750
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger protein 655 (ZNF655) Krueppel C2H2-type zinc-finger protein family Q8N720
Other
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cysteine-rich tail protein 1 (CYSRT1) CYSRT1 family A8MQ03
Protein HEXIM2 (HEXIM2) HEXIM family Q96MH2
Ropporin-1A (ROPN1) Ropporin family Q9HAT0
SAGA-associated factor 29 (SGF29) SGF29 family Q96ES7
Rhombotin-2 (LMO2) . P25791
Sprouty-related, EVH1 domain-containing protein 1 (SPRED1) . Q7Z699

References

1 Global profiling of phosphorylation-dependent changes in cysteine reactivity. Nat Methods. 2022 Mar;19(3):341-352. doi: 10.1038/s41592-022-01398-2. Epub 2022 Feb 28.
Mass spectrometry data entry: PXD026730
2 Differently Tagged Probes for Protein Profiling of Mitochondria. Chembiochem. 2019 May 2;20(9):1155-1160. doi: 10.1002/cbic.201800735. Epub 2019 Mar 26.