Details of the Target

General Information of Target

Target ID LDTP08851
Target Name Polypeptide N-acetylgalactosaminyltransferase 16 (GALNT16)
Gene Name GALNT16
Gene ID 57452
Synonyms
GALNTL1; KIAA1130; Polypeptide N-acetylgalactosaminyltransferase 16; EC 2.4.1.41; Polypeptide GalNAc transferase 16; GalNAc-T16; Polypeptide GalNAc transferase-like protein 1; GalNAc-T-like protein 1; pp-GaNTase-like protein 1; Polypeptide N-acetylgalactosaminyltransferase-like protein 1; Protein-UDP acetylgalactosaminyltransferase-like protein 1; UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-like protein 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRKIRANAIAILTVAWILGTFYYLWQDNRAHAASSGGRGAQRAGRRSEQLREDRTIPLIV
TGTPSKGFDEKAYLSAKQLKAGEDPYRQHAFNQLESDKLSPDRPIRDTRHYSCPSVSYSS
DLPATSVIITFHNEARSTLLRTVKSVLNRTPANLIQEIILVDDFSSDPEDCLLLTRIPKV
KCLRNDRREGLIRSRVRGADVAAATVLTFLDSHCEVNTEWLPPMLQRVKEDHTRVVSPII
DVISLDNFAYLAASADLRGGFDWSLHFKWEQIPLEQKMTRTDPTRPIRTPVIAGGIFVID
KSWFNHLGKYDAQMDIWGGENFELSFRVWMCGGSLEIVPCSRVGHVFRKRHPYNFPEGNA
LTYIRNTKRTAEVWMDEYKQYYYEARPSAIGKAFGSVATRIEQRKKMNCKSFRWYLENVY
PELTVPVKEALPGIIKQGVNCLESQGQNTAGDFLLGMGICRGSAKNPQPAQAWLFSDHLI
QQQGKCLAATSTLMSSPGSPVILQMCNPREGKQKWRRKGSFIQHSVSGLCLETKPAQLVT
SKCQADAQAQQWQLLPHT
Target Bioclass
Enzyme
Family
Glycosyltransferase 2 family, GalNAc-T subfamily
Subcellular location
Golgi apparatus membrane
Function Catalyzes the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.
Uniprot ID
Q8N428
Ensemble ID
ENST00000337827.8
HGNC ID
HGNC:23233

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO678 SNV: p.L173I .
COLO679 SNV: p.F91S .
LNCaP clone FGC SNV: p.V127I .
MFE319 SNV: p.R327G .
MOLM16 SNV: p.N359I .
NCIH1048 Deletion: p.G319EfsTer83 .
NUGC3 Deletion: p.G319EfsTer83 .
OCUM1 SNV: p.R386W .
OVCAR8 SNV: p.V228G .
RKO SNV: p.S137F .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Acrolein
 Probe Info  		N.A.  LDD0223  [1]
DBIA
 Probe Info  		C331(0.92); C340(0.92)  LDD0078  [2]
NAIA_4
 Probe Info  		N.A.  LDD2226  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0237  AC12 HEK-293T C214(1.27)  LDD1510  [4]
 LDCM0280  AC20 HEK-293T C214(1.13)  LDD1519  [4]
 LDCM0288  AC28 HEK-293T C214(1.10)  LDD1527  [4]
 LDCM0297  AC36 HEK-293T C214(1.10)  LDD1536  [4]
 LDCM0301  AC4 HEK-293T C214(1.05)  LDD1540  [4]
 LDCM0306  AC44 HEK-293T C214(1.03)  LDD1545  [4]
 LDCM0315  AC52 HEK-293T C214(1.10)  LDD1554  [4]
 LDCM0324  AC60 HEK-293T C214(1.16)  LDD1563  [4]
 LDCM0020  ARS-1620 HCC44 C331(0.92); C340(0.92)  LDD0078  [2]
 LDCM0408  CL20 HEK-293T C214(1.37)  LDD1612  [4]
 LDCM0421  CL32 HEK-293T C214(1.13)  LDD1625  [4]
 LDCM0434  CL44 HEK-293T C214(1.16)  LDD1638  [4]
 LDCM0447  CL56 HEK-293T C214(1.28)  LDD1650  [4]
 LDCM0460  CL68 HEK-293T C214(1.21)  LDD1663  [4]
 LDCM0473  CL8 HEK-293T C214(1.02)  LDD1676  [4]
 LDCM0474  CL80 HEK-293T C214(1.26)  LDD1677  [4]
 LDCM0487  CL92 HEK-293T C214(1.11)  LDD1690  [4]
 LDCM0109  NEM HeLa N.A.  LDD0223  [1]

References

1 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402