Details of the Target

General Information of Target

Target ID LDTP08763
Target Name Homeobox protein SIX5 (SIX5)
Gene Name SIX5
Gene ID 147912
Synonyms
DMAHP; Homeobox protein SIX5; DM locus-associated homeodomain protein; Sine oculis homeobox homolog 5
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MATLPAEPSAGPAAGGEAVAAAAATEEEEEEARQLLQTLQAAEGEAAAAAGAGAGAAAAG
AEGPGSPGVPGSPPEAASEPPTGLRFSPEQVACVCEALLQAGHAGRLSRFLGALPPAERL
RGSDPVLRARALVAFQRGEYAELYRLLESRPFPAAHHAFLQDLYLRARYHEAERARGRAL
GAVDKYRLRKKFPLPKTIWDGEETVYCFKERSRAALKACYRGNRYPTPDEKRRLATLTGL
SLTQVSNWFKNRRQRDRTGAGGGAPCKSESDGNPTTEDESSRSPEDLERGAAPVSAEAAA
QGSIFLAGTGPPAPCPASSSILVNGSFLAASGSPAVLLNGGPVIINGLALGEASSLGPLL
LTGGGGAPPPQPSPQGASETKTSLVLDPQTGEVRLEEAQSEAPETKGAQVAAPGPALGEE
VLGPLAQVVPGPPTAATFPLPPGPVPAVAAPQVVPLSPPPGYPTGLSPTSPLLNLPQVVP
TSQVVTLPQAVGPLQLLAAGPGSPVKVAAAAGPANVHLINSGVGVTALQLPSATAPGNFL
LANPVSGSPIVTGVALQQGKIILTATFPTSMLVSQVLPPAPGLALPLKPETAISVPEGGL
PVAPSPALPEAHALGTLSAQQPPPAAATTSSTSLPFSPDSPGLLPNFPAPPPEGLMLSPA
AVPVWSAGLELSAGTEGLLEAEKGLGTQAPHTVLRLPDPDPEGLLLGATAGGEVDEGLEA
EAKVLTQLQSVPVEEPLEL
Target Bioclass
Transcription factor
Family
SIX/Sine oculis homeobox family
Subcellular location
Cytoplasm
Function
Transcription factor that is thought to be involved in regulation of organogenesis. May be involved in determination and maintenance of retina formation. Binds a 5'-GGTGTCAG-3' motif present in the ARE regulatory element of ATP1A1. Binds a 5'-TCA[AG][AG]TTNC-3' motif present in the MEF3 element in the myogenin promoter, and in the IGFBP5 promoter. Thought to be regulated by association with Dach and Eya proteins, and seems to be coactivated by EYA1, EYA2 and EYA3.
Uniprot ID
Q8N196
Ensemble ID
ENST00000317578.7
HGNC ID
HGNC:10891

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
AZ-9
 Probe Info  		E285(0.73); E288(1.03); D286(1.18)  LDD2208  [1]
AHL-Pu-1
 Probe Info  		C207(2.04)  LDD0170  [2]
DBIA
 Probe Info  		C93(2.10); C95(2.10)  LDD0080  [3]
NAIA_5
 Probe Info  		C207(0.00); C229(0.00)  LDD2224  [4]
IPM
 Probe Info  		N.A.  LDD0147  [5]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA DM93 C207(2.04)  LDD0170  [2]
 LDCM0226  AC11 HEK-293T C266(1.10)  LDD1509  [7]
 LDCM0278  AC19 HEK-293T C266(1.55)  LDD1517  [7]
 LDCM0287  AC27 HEK-293T C266(1.12)  LDD1526  [7]
 LDCM0290  AC3 HEK-293T C266(1.04)  LDD1529  [7]
 LDCM0296  AC35 HEK-293T C266(1.17)  LDD1535  [7]
 LDCM0305  AC43 HEK-293T C266(1.12)  LDD1544  [7]
 LDCM0314  AC51 HEK-293T C266(1.03)  LDD1553  [7]
 LDCM0322  AC59 HEK-293T C266(1.03)  LDD1561  [7]
 LDCM0020  ARS-1620 HCC44 C93(1.25); C95(1.25)  LDD2171  [3]
 LDCM0406  CL19 HEK-293T C266(1.18)  LDD1610  [7]
 LDCM0420  CL31 HEK-293T C266(1.09)  LDD1624  [7]
 LDCM0433  CL43 HEK-293T C266(1.07)  LDD1637  [7]
 LDCM0446  CL55 HEK-293T C266(1.14)  LDD1649  [7]
 LDCM0459  CL67 HEK-293T C266(1.15)  LDD1662  [7]
 LDCM0462  CL7 HEK-293T C266(1.08)  LDD1665  [7]
 LDCM0472  CL79 HEK-293T C266(1.17)  LDD1675  [7]
 LDCM0486  CL91 HEK-293T C266(1.13)  LDD1689  [7]
 LDCM0022  KB02 HCT 116 C93(2.10); C95(2.10)  LDD0080  [3]
 LDCM0023  KB03 HCT 116 C93(9.76); C95(9.76)  LDD0081  [3]
 LDCM0024  KB05 HCT 116 C93(3.92); C95(3.92)  LDD0082  [3]
 LDCM0021  THZ1 HCT 116 C93(1.25); C95(1.25)  LDD2173  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ataxin-1 (ATXN1) ATXN1 family P54253

References

1 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
2 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
3 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
6 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402