Details of the Target

General Information of Target

Target ID LDTP08753
Target Name Adenylosuccinate synthetase isozyme 1 (ADSS1)
Gene Name ADSS1
Gene ID 122622
Synonyms
ADSSL1; Adenylosuccinate synthetase isozyme 1; AMPSase 1; AdSS 1; EC 6.3.4.4; Adenylosuccinate synthetase, basic isozyme; Adenylosuccinate synthetase, muscle isozyme; M-type adenylosuccinate synthetase; Adenylosuccinate synthetase-like 1; AdSSL1; IMP--aspartate ligase 1
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MSGTRASNDRPPGAGGVKRGRLQQEAAATGSRVTVVLGAQWGDEGKGKVVDLLATDADII
SRCQGGNNAGHTVVVDGKEYDFHLLPSGIINTKAVSFIGNGVVIHLPGLFEEAEKNEKKG
LKDWEKRLIISDRAHLVFDFHQAVDGLQEVQRQAQEGKNIGTTKKGIGPTYSSKAARTGL
RICDLLSDFDEFSSRFKNLAHQHQSMFPTLEIDIEGQLKRLKGFAERIRPMVRDGVYFMY
EALHGPPKKILVEGANAALLDIDFGTYPFVTSSNCTVGGVCTGLGIPPQNIGDVYGVVKA
YTTRVGIGAFPTEQINEIGGLLQTRGHEWGVTTGRKRRCGWLDLMILRYAHMVNGFTALA
LTKLDILDVLGEVKVGVSYKLNGKRIPYFPANQEMLQKVEVEYETLPGWKADTTGARRWE
DLPPQAQNYIRFVENHVGVAVKWVGVGKSRESMIQLF
Target Bioclass
Enzyme
Family
Adenylosuccinate synthetase family
Subcellular location
Cytoplasm
Function
Component of the purine nucleotide cycle (PNC), which interconverts IMP and AMP to regulate the nucleotide levels in various tissues, and which contributes to glycolysis and ammoniagenesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.
Uniprot ID
Q8N142
Ensemble ID
ENST00000330877.7
HGNC ID
HGNC:20093

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
SKMEL30 SNV: p.G20E .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C63(2.48)  LDD3319  [1]
Acrolein
 Probe Info  		N.A.  LDD0233  [2]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [3]
IPM
 Probe Info  		N.A.  LDD2156  [4]
NAIA_5
 Probe Info  		C63(0.00); C183(0.00)  LDD2223  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0270  AC15 HEK-293T C339(1.23)  LDD1513  [6]
 LDCM0283  AC23 HEK-293T C339(1.79)  LDD1522  [6]
 LDCM0292  AC31 HEK-293T C339(1.37)  LDD1531  [6]
 LDCM0300  AC39 HEK-293T C339(1.15)  LDD1539  [6]
 LDCM0309  AC47 HEK-293T C339(1.32)  LDD1548  [6]
 LDCM0318  AC55 HEK-293T C339(1.25)  LDD1557  [6]
 LDCM0327  AC63 HEK-293T C339(1.29)  LDD1566  [6]
 LDCM0334  AC7 HEK-293T C339(1.07)  LDD1568  [6]
 LDCM0632  CL-Sc Hep-G2 C63(1.60)  LDD2227  [5]
 LDCM0379  CL11 HEK-293T C339(1.28)  LDD1583  [6]
 LDCM0411  CL23 HEK-293T C339(1.69)  LDD1615  [6]
 LDCM0424  CL35 HEK-293T C339(1.47)  LDD1628  [6]
 LDCM0437  CL47 HEK-293T C339(1.74)  LDD1641  [6]
 LDCM0450  CL59 HEK-293T C339(1.43)  LDD1653  [6]
 LDCM0464  CL71 HEK-293T C339(1.59)  LDD1667  [6]
 LDCM0477  CL83 HEK-293T C339(1.53)  LDD1680  [6]
 LDCM0490  CL95 HEK-293T C339(1.14)  LDD1693  [6]
 LDCM0022  KB02 22RV1 C63(1.36)  LDD2243  [1]
 LDCM0023  KB03 22RV1 C63(1.81)  LDD2660  [1]
 LDCM0024  KB05 MEWO C63(2.48)  LDD3319  [1]
 LDCM0109  NEM HeLa N.A.  LDD0233  [2]

The Interaction Atlas With This Target

The Drug(s) Related To This Target

Approved
Click To Hide/Show 2 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Acyclovir Small molecular drug DB00787
Aspartic Acid Small molecular drug DB00128
Investigative
Click To Hide/Show 7 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
6-o-phosphoryl Inosine Monophosphate Small molecular drug DB03510
Guanosine-5'-diphosphate Small molecular drug DB04315
Guanosine-5'-triphosphate Small molecular drug DB04137
Hadacidin Small molecular drug DB02109
Inosinic Acid Small molecular drug DB04566
Adenylosuccinic Acid . DB04418
Alanosine . DB05540

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
5 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402