Details of the Target | Chem(Pro)²

General Information of Target

Target ID	LDTP08685
Target Name	Ras and EF-hand domain-containing protein (RASEF)
Gene Name	RASEF
Gene ID	158158
Synonyms	RAB45; Ras and EF-hand domain-containing protein; Ras-related protein Rab-45
3D Structure	Download 2D Sequence (FASTA) Download 3D Structure (PDB) Download
Sequence	MEADGDGEELARLRSVFAACDANRSGRLEREEFRALCTELRVRPADAEAVFQRLDADRDG AITFQEFARGFLGSLRGGRRRDWGPLDPAPAVSEAGPETHDSEEDEGDEDAAAALATSCG PASPGRAWQDFQARLGDEAKFIPREEQVSTLYQNINLVEPRLIQPYEHVIKNFIREIRLQ STEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEHKTRKDEKRKAEEALSDLRRQYE TEVGDLQVTIKKLRKLEEQSKRVSQKEDVAALKKQIYDLSMENQKVKKDLLEAQTNIAFL QSELDALKSDYADQSLNTERDLEIIRAYTEDRNSLERQIEILQTANRKLHDSNDGLRSAL ENSYSKFNRSLHINNISPGNTISRSSPKFIGHSPQPLGYDRSSRSSYVDEDCDSLALCDP LQRTNCEVDSLPESCFDSGLSTLRDPNEYDSEVEYKHQRGFQRSHGVQESFGGDASDTDV PDIRDEETFGLEDVASVLDWKPQGSVSEGSIVSSSRKPISALSPQTDLVDDNAKSFSSQK AYKIVLAGDAAVGKSSFLMRLCKNEFRENISATLGVDFQMKTLIVDGERTVLQLWDTAGQ ERFRSIAKSYFRKADGVLLLYDVTCEKSFLNIREWVDMIEDAAHETVPIMLVGNKADIRD TAATEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSI TNLTGTNSKKSPQMKNCCNG
Target Bioclass	Enzyme
Family	Small GTPase superfamily, Rab family
Subcellular location	Cytoplasm, perinuclear region
Function	Binds predominantly GDP, and also GTP. Acts as a dynein adapter protein that activates dynein-mediated transport and dynein-dynactin motility on microtubules.
Uniprot ID	Q8IZ41
Ensemble ID	ENST00000340717.4
HGNC ID	HGNC:26464

Target Site Mutations in Different Cell Lines

Cell line	Mutation details			Probe for labeling this protein in this cell
AU565	SNV: p.S25L			.
KP4	SNV: p.D531H			.
KYSE180	SNV: p.M209I			.
LNCaP clone FGC	SNV: p.F686L			.
MCC13	SNV: p.E601K			.
MEWO	SNV: p.H372Q; p.K709I			.
NALM6	SNV: p.A194V			.
SNU869	SNV: p.H644Q			.
SSP25	SNV: p.Q247P			.
T98G	SNV: p.L202S			.

Probe(s) Labeling This Target

ABPP Probe

Click To Hide/Show 3 Probe Related to This Target

Probe name		Structure	Binding Site(Ratio)	Interaction ID	Ref
DBIA Probe Info			C426(1.16); C435(1.16)	LDD0078	[1]
NAIA_5 Probe Info			N.A.	LDD2224	[2]
YN-1 Probe Info			N346(0.00); R347(0.00)	LDD0446	[3]

Competitor(s) Related to This Target

Competitor ID	Name	Cell line	Binding Site(Ratio)	Interaction ID	Ref
LDCM0020	ARS-1620	HCC44	C426(1.16); C435(1.16)	LDD0078	[1]

References

1	Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2	N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w. Mass spectrometry data entry: PXD041264
3	Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.