Details of the Target

General Information of Target

Target ID LDTP08661
Target Name E3 ubiquitin-protein ligase TRIM22 (TRIM22)
Gene Name TRIM22
Gene ID 10346
Synonyms
RNF94; STAF50; E3 ubiquitin-protein ligase TRIM22; EC 2.3.2.27; 50 kDa-stimulated trans-acting factor; RING finger protein 94; RING-type E3 ubiquitin transferase TRIM22; Staf-50; Tripartite motif-containing protein 22
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDFSVKVDIEKEVTCPICLELLTEPLSLDCGHSFCQACITAKIKESVIISRGESSCPVCQ
TRFQPGNLRPNRHLANIVERVKEVKMSPQEGQKRDVCEHHGKKLQIFCKEDGKVICWVCE
LSQEHQGHQTFRINEVVKECQEKLQVALQRLIKEDQEAEKLEDDIRQERTAWKNYIQIER
QKILKGFNEMRVILDNEEQRELQKLEEGEVNVLDNLAAATDQLVQQRQDASTLISDLQRR
LRGSSVEMLQDVIDVMKRSESWTLKKPKSVSKKLKSVFRVPDLSGMLQVLKELTDVQYYW
VDVMLNPGSATSNVAISVDQRQVKTVRTCTFKNSNPCDFSAFGVFGCQYFSSGKYYWEVD
VSGKIAWILGVHSKISSLNKRKSSGFAFDPSVNYSKVYSRYRPQYGYWVIGLQNTCEYNA
FEDSSSSDPKVLTLFMAVPPCRIGVFLDYEAGIVSFFNVTNHGALIYKFSGCRFSRPAYP
YFNPWNCLVPMTVCPPSS
Target Bioclass
Enzyme
Family
TRIM/RBCC family
Subcellular location
Cytoplasm
Function
Interferon-induced E3 ubiquitin ligase that plays important roles in innate and adaptive immunity. Restricts the replication of many viruses including HIV-1, encephalomyocarditis virus (EMCV), hepatitis B virus (HBV), hepatitis C virus (HCV) or Zika virus (ZIKV). Mechanistically, negatively regulates HCV replication by promoting ubiquitination and subsequent degradation of viral NS5A. Acts also by promoting the degradation of Zika virus NS1 and NS3 proteins through proteasomal degradation. Acts as a suppressor of basal HIV-1 LTR-driven transcription by preventing Sp1 binding to the HIV-1 promoter. Plays also a role in antiviral immunity by co-regulating together with NT5C2 the RIGI/NF-kappa-B pathway by promoting 'Lys-63'-linked ubiquitination of RIGI, while NT5C2 is responsible for 'Lys-48'-linked ubiquitination of RIGI. Participates in adaptive immunity by suppressing the amount of MHC class II protein in a negative feedback manner in order to limit the extent of MHC class II induction.
Uniprot ID
Q8IYM9
Ensemble ID
ENST00000379965.8
HGNC ID
HGNC:16379

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A549 SNV: p.W357Ter .
AN3CA SNV: p.D338N .
CAL148 SNV: p.D302H .
HCT15 SNV: p.G112E .
MCC26 SNV: p.N306S .
MFE296 SNV: p.C35Y .
PEO1 Deletion: p.K354RfsTer4 .
PF382 SNV: p.W367Ter .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C441(2.09)  LDD3339  [1]
IA-alkyne
 Probe Info  		C416(0.00); C97(0.00)  LDD0167  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C140(1.39); C441(0.63)  LDD2187  [3]
 LDCM0572  Fragment10 Ramos C140(0.70)  LDD2189  [3]
 LDCM0573  Fragment11 Ramos C140(4.97); C441(0.42)  LDD2190  [3]
 LDCM0574  Fragment12 Ramos C140(0.58)  LDD2191  [3]
 LDCM0576  Fragment14 Ramos C140(2.06); C441(0.78)  LDD2193  [3]
 LDCM0579  Fragment20 Ramos C140(0.51)  LDD2194  [3]
 LDCM0582  Fragment23 Ramos C140(1.06)  LDD2196  [3]
 LDCM0586  Fragment28 Ramos C140(0.24)  LDD2198  [3]
 LDCM0588  Fragment30 Ramos C140(1.89)  LDD2199  [3]
 LDCM0589  Fragment31 Ramos C140(1.50)  LDD2200  [3]
 LDCM0590  Fragment32 Ramos C140(0.92)  LDD2201  [3]
 LDCM0468  Fragment33 Ramos C140(1.43)  LDD2202  [3]
 LDCM0596  Fragment38 Ramos C140(1.22)  LDD2203  [3]
 LDCM0566  Fragment4 Ramos C140(0.83); C441(0.47)  LDD2184  [3]
 LDCM0614  Fragment56 Ramos C140(1.02)  LDD2205  [3]
 LDCM0569  Fragment7 Ramos C140(0.73); C441(0.41)  LDD2186  [3]
 LDCM0571  Fragment9 Ramos C140(0.55)  LDD2188  [3]
 LDCM0022  KB02 T cell-activated C337, C347(5.09)  LDD1708  [4]
 LDCM0023  KB03 Ramos C140(1.09); C441(0.40)  LDD2183  [3]
 LDCM0024  KB05 NALM-6 C441(2.09)  LDD3339  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Pleckstrin homology domain-containing family F member 2 (PLEKHF2) . Q9H8W4

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
4 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.