Details of the Target

General Information of Target

Target ID LDTP08531
Target Name Choline transporter-like protein 2 (SLC44A2)
Gene Name SLC44A2
Gene ID 57153
Synonyms
CTL2; Choline transporter-like protein 2; Solute carrier family 44 member 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGDERPHYYGKHGTPQKYDPTFKGPIYNRGCTDIICCVFLLLAIVGYVAVGIIAWTHGDP
RKVIYPTDSRGEFCGQKGTKNENKPYLFYFNIVKCASPLVLLEFQCPTPQICVEKCPDRY
LTYLNARSSRDFEYYKQFCVPGFKNNKGVAEVLQDGDCPAVLIPSKPLARRCFPAIHAYK
GVLMVGNETTYEDGHGSRKNITDLVEGAKKANGVLEARQLAMRIFEDYTVSWYWIIIGLV
IAMAMSLLFIILLRFLAGIMVWVMIIMVILVLGYGIFHCYMEYSRLRGEAGSDVSLVDLG
FQTDFRVYLHLRQTWLAFMIILSILEVIIILLLIFLRKRILIAIALIKEASRAVGYVMCS
LLYPLVTFFLLCLCIAYWASTAVFLSTSNEAVYKIFDDSPCPFTAKTCNPETFPSSNESR
QCPNARCQFAFYGGESGYHRALLGLQIFNAFMFFWLANFVLALGQVTLAGAFASYYWALR
KPDDLPAFPLFSAFGRALRYHTGSLAFGALILAIVQIIRVILEYLDQRLKAAENKFAKCL
MTCLKCCFWCLEKFIKFLNRNAYIMIAIYGTNFCTSARNAFFLLMRNIIRVAVLDKVTDF
LFLLGKLLIVGSVGILAFFFFTHRIRIVQDTAPPLNYYWVPILTVIVGSYLIAHGFFSVY
GMCVDTLFLCFLEDLERNDGSAERPYFMSSTLKKLLNKTNKKAAES
Target Bioclass
Transporter and channel
Family
CTL (choline transporter-like) family
Subcellular location
Membrane
Function
[Isoform 1]: Choline/H+ antiporter, mainly in mitochodria. Also acts as a low-affinity ethanolamine/H+ antiporter, regulating the supply of extracellular ethanolamine (Etn) for the CDP-Etn pathway, redistribute intracellular Etn and balance the CDP-Cho and CDP-Etn arms of the Kennedy pathway.; [Isoform 3]: Does not exhibit choline transporter activity.
Uniprot ID
Q8IWA5
Ensemble ID
ENST00000335757.10
HGNC ID
HGNC:17292

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
JURKAT SNV: p.T14M; p.Q16Ter .
MOLT4 Insertion: p.G24RfsTer6
SNV: p.A425V;p.G570D		 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
OPA-S-S-alkyne
 Probe Info  		K62(2.64); K210(2.80)  LDD3494  [1]
Probe 1
 Probe Info  		Y65(148.86)  LDD3495  [2]
DBIA
 Probe Info  		C401(1.81)  LDD3310  [3]
Acrolein
 Probe Info  		N.A.  LDD0222  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [5]
STPyne
 Probe Info  		N.A.  LDD0009  [6]
NAIA_5
 Probe Info  		C427(0.00); C172(0.00)  LDD2223  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C401(0.82)  LDD1508  [8]
 LDCM0277  AC18 HEK-293T C401(1.25)  LDD1516  [8]
 LDCM0279  AC2 HEK-293T C401(0.91)  LDD1518  [8]
 LDCM0286  AC26 HEK-293T C401(1.24)  LDD1525  [8]
 LDCM0295  AC34 HEK-293T C401(0.88)  LDD1534  [8]
 LDCM0304  AC42 HEK-293T C401(0.90)  LDD1543  [8]
 LDCM0313  AC50 HEK-293T C401(0.86)  LDD1552  [8]
 LDCM0321  AC58 HEK-293T C401(0.93)  LDD1560  [8]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [4]
 LDCM0369  CL100 HEK-293T C401(1.16)  LDD1573  [8]
 LDCM0371  CL102 HEK-293T C401(0.76)  LDD1575  [8]
 LDCM0373  CL104 HEK-293T C401(0.93)  LDD1577  [8]
 LDCM0375  CL106 HEK-293T C401(0.72)  LDD1579  [8]
 LDCM0377  CL108 HEK-293T C401(0.96)  LDD1581  [8]
 LDCM0380  CL110 HEK-293T C401(0.77)  LDD1584  [8]
 LDCM0382  CL112 HEK-293T C401(1.05)  LDD1586  [8]
 LDCM0384  CL114 HEK-293T C401(0.78)  LDD1588  [8]
 LDCM0386  CL116 HEK-293T C401(1.02)  LDD1590  [8]
 LDCM0388  CL118 HEK-293T C401(0.84)  LDD1592  [8]
 LDCM0391  CL120 HEK-293T C401(1.07)  LDD1595  [8]
 LDCM0393  CL122 HEK-293T C401(0.87)  LDD1597  [8]
 LDCM0395  CL124 HEK-293T C401(1.24)  LDD1599  [8]
 LDCM0397  CL126 HEK-293T C401(0.86)  LDD1601  [8]
 LDCM0399  CL128 HEK-293T C401(1.04)  LDD1603  [8]
 LDCM0401  CL14 HEK-293T C401(1.02)  LDD1605  [8]
 LDCM0403  CL16 HEK-293T C401(1.13)  LDD1607  [8]
 LDCM0405  CL18 HEK-293T C401(0.99)  LDD1609  [8]
 LDCM0407  CL2 HEK-293T C401(0.83)  LDD1611  [8]
 LDCM0414  CL26 HEK-293T C401(0.83)  LDD1618  [8]
 LDCM0416  CL28 HEK-293T C401(1.39)  LDD1620  [8]
 LDCM0419  CL30 HEK-293T C401(0.94)  LDD1623  [8]
 LDCM0429  CL4 HEK-293T C401(1.03)  LDD1633  [8]
 LDCM0430  CL40 HEK-293T C401(1.05)  LDD1634  [8]
 LDCM0432  CL42 HEK-293T C401(0.88)  LDD1636  [8]
 LDCM0441  CL50 HEK-293T C401(0.73)  LDD1645  [8]
 LDCM0443  CL52 HEK-293T C401(1.14)  LDD1646  [8]
 LDCM0445  CL54 HEK-293T C401(0.89)  LDD1648  [8]
 LDCM0451  CL6 HEK-293T C401(0.91)  LDD1654  [8]
 LDCM0454  CL62 HEK-293T C401(0.87)  LDD1657  [8]
 LDCM0456  CL64 HEK-293T C401(1.16)  LDD1659  [8]
 LDCM0458  CL66 HEK-293T C401(1.08)  LDD1661  [8]
 LDCM0467  CL74 HEK-293T C401(0.82)  LDD1670  [8]
 LDCM0469  CL76 HEK-293T C401(1.05)  LDD1672  [8]
 LDCM0471  CL78 HEK-293T C401(0.99)  LDD1674  [8]
 LDCM0480  CL86 HEK-293T C401(1.05)  LDD1683  [8]
 LDCM0482  CL88 HEK-293T C401(1.15)  LDD1685  [8]
 LDCM0485  CL90 HEK-293T C401(0.95)  LDD1688  [8]
 LDCM0493  CL98 HEK-293T C401(0.82)  LDD1696  [8]
 LDCM0427  Fragment51 HEK-293T C401(0.81)  LDD1631  [8]
 LDCM0022  KB02 HEK-293T C401(0.91)  LDD1492  [8]
 LDCM0023  KB03 HEK-293T C401(0.86)  LDD1497  [8]
 LDCM0024  KB05 COLO792 C401(1.81)  LDD3310  [3]

The Interaction Atlas With This Target

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Choline Small molecular drug DB00122

References

1 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
2 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
6 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
7 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
8 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402