Details of the Target

General Information of Target

Target ID LDTP08484
Target Name Ras guanyl-releasing protein 3 (RASGRP3)
Gene Name RASGRP3
Gene ID 25780
Synonyms
GRP3; KIAA0846; Ras guanyl-releasing protein 3; Calcium and DAG-regulated guanine nucleotide exchange factor III; Guanine nucleotide exchange factor for Rap1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGSSGLGKAATLDELLCTCIEMFDDNGELDNSYLPRIVLLMHRWYLSSTELAEKLLCMYR
NATGESCNEFRLKICYFMRYWILKFPAEFNLDLGLIRMTEEFREVASQLGYEKHVSLIDI
SSIPSYDWMRRVTQRKKVSKKGKACLLFDHLEPIELAEHLTFLEHKSFRRISFTDYQSYV
IHGCLENNPTLERSIALFNGISKWVQLMVLSKPTPQQRAEVITKFINVAKKLLQLKNFNT
LMAVVGGLSHSSISRLKETHSHLSSEVTKNWNEMTELVSSNGNYCNYRKAFADCDGFKIP
ILGVHLKDLIAVHVIFPDWTEENKVNIVKMHQLSVTLSELVSLQNASHHLEPNMDLINLL
TLSLDLYHTEDDIYKLSLVLEPRNSKSQPTSPTTPNKPVVPLEWALGVMPKPDPTVINKH
IRKLVESVFRNYDHDHDGYISQEDFESIAANFPFLDSFCVLDKDQDGLISKDEMMAYFLR
AKSQLHCKMGPGFIHNFQEMTYLKPTFCEHCAGFLWGIIKQGYKCKDCGANCHKQCKDLL
VLACRRFARAPSLSSGHGSLPGSPSLPPAQDEVFEFPGVTAGHRDLDSRAITLVTGSSRK
ISVRLQRATTSQATQTEPVWSEAGWGDSGSHTFPKMKSKFHDKAAKDKGFAKWENEKPRV
HAGVDVVDRGTEFELDQDEGEETRQDGEDG
Target Bioclass
Other
Family
RASGRP family
Function Guanine nucleotide exchange factor (GEF) for Ras and Rap1.
Uniprot ID
Q8IV61
Ensemble ID
ENST00000402538.7
HGNC ID
HGNC:14545
ChEMBL ID
CHEMBL3638

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
C32 SNV: p.M636I .
DOTC24510 SNV: p.A548T .
DU145 SNV: p.S202F .
EGI1 SNV: p.K166N .
HEC1 SNV: p.Q177K .
HEC1B SNV: p.Q177K .
HT115 SNV: p.R60Q; p.R60Ter; p.N496D .
KYSE30 SNV: p.L15P .
MCC13 SNV: p.E69K; p.M636I .
NCIH1703 SNV: p.R422S .
NCIH2286 Insertion: p.F225VfsTer15 .
OVK18 Deletion: p.Q216SfsTer26 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info  		C544(0.61)  LDD2182  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C544(0.80); C67(0.56)  LDD2187  [1]
 LDCM0572  Fragment10 Ramos C544(0.67); C67(3.47)  LDD2189  [1]
 LDCM0573  Fragment11 Ramos C544(0.28); C67(5.02)  LDD2190  [1]
 LDCM0574  Fragment12 Ramos C544(0.91)  LDD2191  [1]
 LDCM0575  Fragment13 Ramos C544(0.75); C67(1.08)  LDD2192  [1]
 LDCM0576  Fragment14 Ramos C544(1.23)  LDD2193  [1]
 LDCM0579  Fragment20 Ramos C544(0.97)  LDD2194  [1]
 LDCM0580  Fragment21 Ramos C544(0.95); C67(1.79)  LDD2195  [1]
 LDCM0582  Fragment23 Ramos C544(1.16); C67(0.82)  LDD2196  [1]
 LDCM0578  Fragment27 Ramos C544(1.12); C67(0.91)  LDD2197  [1]
 LDCM0586  Fragment28 Ramos C544(0.65); C67(1.05)  LDD2198  [1]
 LDCM0588  Fragment30 Ramos C544(0.85); C67(1.39)  LDD2199  [1]
 LDCM0589  Fragment31 Ramos C544(1.15); C67(1.53)  LDD2200  [1]
 LDCM0590  Fragment32 Ramos C544(0.54); C67(5.25)  LDD2201  [1]
 LDCM0468  Fragment33 Ramos C544(0.64); C67(1.79)  LDD2202  [1]
 LDCM0596  Fragment38 Ramos C544(0.69); C67(1.62)  LDD2203  [1]
 LDCM0566  Fragment4 Ramos C544(1.62)  LDD2184  [1]
 LDCM0610  Fragment52 Ramos C544(0.85); C67(1.76)  LDD2204  [1]
 LDCM0614  Fragment56 Ramos C544(0.86); C67(1.06)  LDD2205  [1]
 LDCM0569  Fragment7 Ramos C544(0.87)  LDD2186  [1]
 LDCM0022  KB02 Ramos C544(0.61)  LDD2182  [1]
 LDCM0023  KB03 Ramos C544(0.95)  LDD2183  [1]
 LDCM0024  KB05 Ramos C544(1.14)  LDD2185  [1]

References

1 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578