Details of the Target

General Information of Target

Target ID LDTP08377
Target Name E3 ubiquitin-protein ligase DTX1 (DTX1)
Gene Name DTX1
Gene ID 1840
Synonyms
E3 ubiquitin-protein ligase DTX1; EC 2.3.2.27; Protein deltex-1; Deltex1; hDTX1; RING-type E3 ubiquitin transferase DTX1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSRPGHGGLMPVNGLGFPPQNVARVVVWEWLNEHSRWRPYTATVCHHIENVLKEDARGSV
VLGQVDAQLVPYIIDLQSMHQFRQDTGTMRPVRRNFYDPSSAPGKGIVWEWENDGGAWTA
YDMDICITIQNAYEKQHPWLDLSSLGFCYLIYFNSMSQMNRQTRRRRRLRRRLDLAYPLT
VGSIPKSQSWPVGASSGQPCSCQQCLLVNSTRAASNAILASQRRKAPPAPPLPPPPPPGG
PPGALAVRPSATFTGAALWAAPAAGPAEPAPPPGAPPRSPGAPGGARTPGQNNLNRPGPQ
RTTSVSARASIPPGVPALPVKNLNGTGPVHPALAGMTGILLCAAGLPVCLTRAPKPILHP
PPVSKSDVKPVPGVPGVCRKTKKKHLKKSKNPEDVVRRYMQKVKNPPDEDCTICMERLVT
ASGYEGVLRHKGVRPELVGRLGRCGHMYHLLCLVAMYSNGNKDGSLQCPTCKAIYGEKTG
TQPPGKMEFHLIPHSLPGFPDTQTIRIVYDIPTGIQGPEHPNPGKKFTARGFPRHCYLPN
NEKGRKVLRLLITAWERRLIFTIGTSNTTGESDTVVWNEIHHKTEFGSNLTGHGYPDASY
LDNVLAELTAQGVSEAAAKA
Target Bioclass
Enzyme
Family
Deltex family
Subcellular location
Cytoplasm
Function
Functions as a ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity. Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it also acts as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Involved in neurogenesis, lymphogenesis and myogenesis, and may also be involved in MZB (Marginal zone B) cell differentiation. Promotes B-cell development at the expense of T-cell development, suggesting that it can antagonize NOTCH1.
Uniprot ID
Q86Y01
Ensemble ID
ENST00000257600.3
HGNC ID
HGNC:3060

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C378(2.17)  LDD3403  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0163  [2]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [3]
NAIA_4
 Probe Info  		N.A.  LDD2226  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C378(1.18)  LDD2187  [5]
 LDCM0572  Fragment10 Ramos C378(0.67)  LDD2189  [5]
 LDCM0573  Fragment11 Ramos C378(0.35)  LDD2190  [5]
 LDCM0574  Fragment12 Ramos C378(0.61)  LDD2191  [5]
 LDCM0575  Fragment13 Ramos C378(0.67)  LDD2192  [5]
 LDCM0576  Fragment14 Ramos C378(0.88)  LDD2193  [5]
 LDCM0579  Fragment20 Ramos C378(0.59)  LDD2194  [5]
 LDCM0580  Fragment21 Ramos C378(0.82)  LDD2195  [5]
 LDCM0582  Fragment23 Ramos C378(0.63)  LDD2196  [5]
 LDCM0578  Fragment27 Ramos C378(0.73)  LDD2197  [5]
 LDCM0586  Fragment28 Ramos C378(0.80)  LDD2198  [5]
 LDCM0588  Fragment30 Ramos C378(1.04)  LDD2199  [5]
 LDCM0589  Fragment31 Ramos C378(0.60)  LDD2200  [5]
 LDCM0590  Fragment32 Ramos C378(0.73)  LDD2201  [5]
 LDCM0468  Fragment33 Ramos C378(0.75)  LDD2202  [5]
 LDCM0596  Fragment38 Ramos C378(0.72)  LDD2203  [5]
 LDCM0566  Fragment4 Ramos C378(1.60)  LDD2184  [5]
 LDCM0610  Fragment52 Ramos C378(0.70)  LDD2204  [5]
 LDCM0614  Fragment56 Ramos C378(0.88)  LDD2205  [5]
 LDCM0569  Fragment7 Ramos C378(1.26)  LDD2186  [5]
 LDCM0571  Fragment9 Ramos C378(0.64)  LDD2188  [5]
 LDCM0022  KB02 Ramos C378(1.49)  LDD2182  [5]
 LDCM0023  KB03 Ramos C378(0.96)  LDD2183  [5]
 LDCM0024  KB05 Reh C378(2.17)  LDD3403  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
cAMP-dependent protein kinase type I-alpha regulatory subunit (PRKAR1A) CAMP-dependent kinase regulatory chain family P10644
Adapter molecule crk (CRK) CRK family P46108
Polyubiquitin-C (UBC) Ubiquitin family P0CG48

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578