Details of the Target

General Information of Target

Target ID LDTP08286
Target Name Kinesin-like protein KIF27 (KIF27)
Gene Name KIF27
Gene ID 55582
Synonyms
Kinesin-like protein KIF27
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEEIPVKVAVRIRPLLCKEALHNHQVCVRVIPNSQQVIIGRDRVFTFDFVFGKNSTQDEV
YNTCIKPLVLSLIEGYNATVFAYGQTGSGKTYTIGGGHIASVVEGQKGIIPRAIQEIFQS
ISEHPSIDFNVKVSYIEVYKEDLRDLLELETSMKDLHIREDEKGNTVIVGAKECHVESAG
EVMSLLEMGNAARHTGTTQMNEHSSRSHAIFTISICQVHKNMEAAEDGSWYSPRHIVSKF
HFVDLAGSERVTKTGNTGERFKESIQINSGLLALGNVISALGDPRRKSSHIPYRDAKITR
LLKDSLGGSAKTVMITCVSPSSSNFDESLNSLKYANRARNIRNKPTVNFSPESDRIDEME
FEIKLLREALQSQQAGVSQTTQINREGSPDTNRIHSLEEQVAQLQGECLGYQCCVEEAFT
FLVDLKDTVRLNEKQQHKLQEWFNMIQEVRKAVLTSFRGIGGTASLEEGPQHVTVLQLKR
ELKKCQCVLAADEVVFNQKELEVKELKNQVQMMVQENKGHAVSLKEAQKVNRLQNEKIIE
QQLLVDQLSEELTKLNLSVTSSAKENCGDGPDARIPERRPYTVPFDTHLGHYIYIPSRQD
SRKVHTSPPMYSLDRIFAGFRTRSQMLLGHIEEQDKVLHCQFSDNSDDEESEGQEKSGTR
CRSRSWIQKPDSVCSLVELSDTQDETQKSDLENEDLKIDCLQESQELNLQKLKNSERILT
EAKQKMRELTINIKMKEDLIKELIKTGNDAKSVSKQYSLKVTKLEHDAEQAKVELIETQK
QLQELENKDLSDVAMKVKLQKEFRKKMDAAKLRVQVLQKKQQDSKKLASLSIQNEKRANE
LEQSVDHMKYQKIQLQRKLREENEKRKQLDAVIKRDQQKIKEIQLKTGQEEGLKPKAEDL
DACNLKRRKGSFGSIDHLQKLDEQKKWLDEEVEKVLNQRQELEELEADLKKREAIVSKKE
ALLQEKSHLENKKLRSSQALNTDSLKISTRLNLLEQELSEKNVQLQTSTAEEKTKISEQV
EVLQKEKDQLQKRRHNVDEKLKNGRVLSPEEEHVLFQLEEGIEALEAAIEYRNESIQNRQ
KSLRASFHNLSRGEANVLEKLACLSPVEIRTILFRYFNKVVNLREAERKQQLYNEEMKMK
VLERDNMVRELESALDHLKLQCDRRLTLQQKEHEQKMQLLLHHFKEQDGEGIMETFKTYE
DKIQQLEKDLYFYKKTSRDHKKKLKELVGEAIRRQLAPSEYQEAGDGVLKPEGGGMLSEE
LKWASRPESMKLSGREREMDSSASSLRTQPNPQKLWEDIPELPPIHSSLAPPSGHMLGNE
NKTETDDNQFTKSHSRLSSQIQVVGNVGRLHGVTPVKLCRKELRQISALELSLRRSSLGV
GIGSMAADSIEVSRKPRDLKT
Target Bioclass
Enzyme
Family
TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, KIF27 subfamily
Subcellular location
Cytoplasm, cytoskeleton
Function Plays an essential role in motile ciliogenesis.
Uniprot ID
Q86VH2
Ensemble ID
ENST00000297814.7
HGNC ID
HGNC:18632
ChEMBL ID
CHEMBL3879867

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
8505C SNV: p.Q654E .
A3KAW SNV: p.D1326H .
AN3CA SNV: p.T1198A .
CASKI Insertion: p.M726NfsTer7 .
GB1 SNV: p.A573V .
HCT116 Deletion: p.K107RfsTer25 .
HCT15 SNV: p.D586Y; p.Q1029Ter .
HT115 SNV: p.K1176T .
KP4 SNV: p.H22Q .
MFE319 SNV: p.K1322E .
NALM6 SNV: p.R664H .
NCIH1155 SNV: p.E150Q .
NCIH1793 SNV: p.E692Ter .
NCIH1993 SNV: p.M188I .
RVH421 SNV: p.E1207K .
SW403 Deletion: p.Q199del
SNV: p.M200V;p.E1018G		 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C1103(2.75)  LDD3423  [1]
Acrolein
 Probe Info  		N.A.  LDD0222  [2]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
VE-P
 Probe Info  		N.A.  LDD0396  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [2]
 LDCM0022  KB02 A673 C1103(1.87)  LDD2261  [1]
 LDCM0023  KB03 A673 C1103(2.47)  LDD2678  [1]
 LDCM0024  KB05 SK-ES1 C1103(2.75)  LDD3423  [1]
 LDCM0109  NEM HeLa N.A.  LDD0230  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Melanoma-associated antigen 3 (MAGEA3) . P43357
Melanoma-associated antigen 6 (MAGEA6) . P43360

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
3 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.