Details of the Target

General Information of Target

Target ID LDTP08251
Target Name Pleckstrin homology-like domain family B member 1 (PHLDB1)
Gene Name PHLDB1
Gene ID 23187
Synonyms
KIAA0638; LL5A; Pleckstrin homology-like domain family B member 1; Protein LL5-alpha
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDALNRNQIGPGCQTQTMVQKGPLDLIETGKGLKVQTDKPHLVSLGSGRLSTAITLLPLE
EGRTVIGSAARDISLQGPGLAPEHCYIENLRGTLTLYPCGNACTIDGLPVRQPTRLTQGC
MLCLGQSTFLRFNHPAEAKWMKSMIPAGGRAPGPPYSPVPAESESLVNGNHTPQTATRGP
SACASHSSLVSSIEKDLQEIMDSLVLEEPGAAGKKPAATSPLSPMANGGRYLLSPPTSPG
AMSVGSSYENTSPAFSPLSSPASSGSCASHSPSGQEPGPSVPPLVPARSSSYHLALQPPQ
SRPSGARSESPRLSRKGGHERPPSPGLRGLLTDSPAATVLAEARRATESPRLGGQLPVVA
ISLSEYPASGALSQPTSIPGSPKFQPPVPAPRNKIGTLQDRPPSPFREPPGSERVLTTSP
SRQLVGRTFSDGLATRTLQPPESPRLGRRGLDSMRELPPLSPSLSRRALSPLPTRTTPDP
KLNREVAESPRPRRWAAHGASPEDFSLTLGARGRRTRSPSPTLGESLAPHKGSFSGRLSP
AYSLGSLTGASPCQSPCVQRKLSSGDLRVPVTRERKNSITEISDNEDDLLEYHRRQRQER
LREQEMERLERQRLETILNLCAEYSRADGGPEAGELPSIGEATAALALAGRRPSRGLAGA
SGRSSEEPGVATQRLWESMERSDEENLKEECSSTESTQQEHEDAPSTKLQGEVLALEEER
AQVLGHVEQLKVRVKELEQQLQESAREAEMERALLQGEREAERALLQKEQKAVDQLQEKL
VALETGIQKERDKEAEALETETKLFEDLEFQQLERESRVEEERELAGQGLLRSKAELLRS
IAKRKERLAILDSQAGQIRAQAVQESERLARDKNASLQLLQKEKEKLTVLERRYHSLTGG
RPFPKTTSTLKEMEKLLLPAVDLEQWYQELMAGLGTGPAAASPHSSPPPLPAKASRQLQV
YRSKMDGEATSPLPRTRSGPLPSSSGSSSSSSQLSVATLGRSPSPKSALLTQNGTGSLPR
NLAATLQDIETKRQLALQQKGQQVIEEQRRRLAELKQKAAAEAQCQWDALHGAAPFPAGP
SGFPPLMHHSILHHLPAGRERGEEGEHAYDTLSLESSDSMETSISTGGNSACSPDNMSSA
SGLDMGKIEEMEKMLKEAHAEKNRLMESREREMELRRQALEEERRRREQVERRLQSESAR
RQQLVEKEVKMREKQFSQARPLTRYLPIRKEDFDLKTHIESSGHGVDTCLHVVLSSKVCR
GYLVKMGGKIKSWKKRWFVFDRLKRTLSYYVDKHETKLKGVIYFQAIEEVYYDHLRSAAK
KRFFRFTMVTESPNPALTFCVKTHDRLYYMVAPSAEAMRIWMDVIVTGAEGYTQFMN
Target Bioclass
Other
Uniprot ID
Q86UU1
Ensemble ID
ENST00000356063.9
HGNC ID
HGNC:23697

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
ABC1 SNV: p.S506F .
AGS SNV: p.L1235R .
D283MED SNV: p.S638R .
DETROIT562 SNV: p.L352R .
DU145 SNV: p.S625N .
HCC1954 SNV: p.S1002G .
HCT116 SNV: p.E1182G; p.T1237I .
HCT15 SNV: p.S68A; p.G967D; p.Q1218H .
HT SNV: p.L116R; p.Y592C .
Ishikawa (Heraklio) 02 ER SNV: p.Y624H .
JURKAT SNV: p.K1207N .
LS180 Deletion: p.L981SfsTer55 .
MFE296 SNV: p.S1123N .
NB1 SNV: p.E1181A .
NB4 SNV: p.E488G .
NCIH2291 SNV: p.R594Q .
NCIH358 SNV: p.T998N .
RKO Deletion: p.N577IfsTer42 .
SH4 SNV: p.A1061V .
SKMEL24 SNV: p.P389S .
SUPT1 SNV: p.A336T .
SW403 SNV: p.E1191Ter .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
STPyne
 Probe Info  		K1032(0.98); K1293(5.88); K779(10.00)  LDD0277  [2]
AHL-Pu-1
 Probe Info  		C691(2.50)  LDD0171  [3]
DBIA
 Probe Info  		C553(0.99); C557(0.99); C691(0.99)  LDD0078  [4]
ATP probe
 Probe Info  		N.A.  LDD0199  [5]
NAIA_4
 Probe Info  		N.A.  LDD2226  [6]
NAIA_5
 Probe Info  		C183(0.00); C691(0.00)  LDD2225  [6]
IPM
 Probe Info  		C183(0.00); C553(0.00)  LDD0147  [7]
AOyne
 Probe Info  		15.00  LDD0443  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0026  4SU-RNA+native RNA DM93 C691(2.50)  LDD0171  [3]
 LDCM0156  Aniline NCI-H1299 13.67  LDD0403  [1]
 LDCM0020  ARS-1620 HCC44 C553(0.99); C557(0.99); C691(0.99)  LDD0078  [4]
 LDCM0632  CL-Sc Hep-G2 C691(20.00)  LDD2227  [6]
 LDCM0367  CL1 HEK-293T C183(0.97)  LDD1571  [9]
 LDCM0370  CL101 HEK-293T C183(1.36)  LDD1574  [9]
 LDCM0374  CL105 HEK-293T C183(1.21)  LDD1578  [9]
 LDCM0378  CL109 HEK-293T C183(1.16)  LDD1582  [9]
 LDCM0383  CL113 HEK-293T C183(1.32)  LDD1587  [9]
 LDCM0387  CL117 HEK-293T C183(1.23)  LDD1591  [9]
 LDCM0392  CL121 HEK-293T C183(1.39)  LDD1596  [9]
 LDCM0396  CL125 HEK-293T C183(0.96)  LDD1600  [9]
 LDCM0400  CL13 HEK-293T C183(1.37)  LDD1604  [9]
 LDCM0413  CL25 HEK-293T C183(1.16)  LDD1617  [9]
 LDCM0426  CL37 HEK-293T C183(1.16)  LDD1630  [9]
 LDCM0439  CL49 HEK-293T C183(0.92)  LDD1643  [9]
 LDCM0453  CL61 HEK-293T C183(0.89)  LDD1656  [9]
 LDCM0466  CL73 HEK-293T C183(1.19)  LDD1669  [9]
 LDCM0479  CL85 HEK-293T C183(0.89)  LDD1682  [9]
 LDCM0492  CL97 HEK-293T C183(0.93)  LDD1695  [9]
 LDCM0022  KB02 769-P C85(1.01); C1340(1.81)  LDD2246  [10]
 LDCM0023  KB03 769-P C85(1.35); C1340(2.21)  LDD2663  [10]
 LDCM0024  KB05 OAW-42 C13(1.61)  LDD3369  [10]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
RNA-binding protein FXR1 (FXR1) FMR1 family P51114

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
4 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
5 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
8 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
9 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
10 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840