Details of the Target

General Information of Target

Target ID LDTP08120
Target Name Rho GTPase-activating protein 30 (ARHGAP30)
Gene Name ARHGAP30
Gene ID 257106
Synonyms
Rho GTPase-activating protein 30; Rho-type GTPase-activating protein 30
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MKSRQKGKKKGSAKERVFGCDLQEHLQHSGQEVPQVLKSCAEFVEEYGVVDGIYRLSGVS
SNIQKLRQEFESERKPDLRRDVYLQDIHCVSSLCKAYFRELPDPLLTYRLYDKFAEAVGV
QLEPERLVKILEVLRELPVPNYRTLEFLMRHLVHMASFSAQTNMHARNLAIVWAPNLLRS
KDIEASGFNGTAAFMEVRVQSIVVEFILTHVDQLFGGAALSGGEVESGWRSLPGTRASGS
PEDLMPRPLPYHLPSILQAGDGPPQMRPYHTIIEIAEHKRKGSLKVRKWRSIFNLGRSGH
ETKRKLPRGAEDREDKSNKGTLRPAKSMDSLSAAAGASDEPEGLVGPSSPRPSPLLPESL
ENDSIEAAEGEQEPEAEALGGTNSEPGTPRAGRSAIRAGGSSRAERCAGVHISDPYNVNL
PLHITSILSVPPNIISNVSLARLTRGLECPALQHRPSPASGPGPGPGLGPGPPDEKLEAS
PASSPLADSGPDDLAPALEDSLSQEVQDSFSFLEDSSSSEPEWVGAEDGEVAQAEAAGAA
FSPGEDDPGMGYLEELLGVGPQVEEFSVEPPLDDLSLDEAQFVLAPSCCSLDSAGPRPEV
EEENGEEVFLSAYDDLSPLLGPKPPIWKGSGSLEGEAAGCGRQALGQGGEEQACWEVGED
KQAEPGGRLDIREEAEGSPETKVEAGKASEDRGEAGGSQETKVRLREGSREETEAKEEKS
KGQKKADSMEAKGVEEPGGDEYTDEKEKEIEREEDEQREEAQVEAGRDLEQGAQEDQVAE
EKWEVVQKQEAEGVREDEDKGQREKGYHEARKDQGDGEDSRSPEAATEGGAGEVSKERES
GDGEAEGDQRAGGYYLEEDTLSEGSGVASLEVDCAKEGNPHSSEMEEVAPQPPQPEEMEP
EGQPSPDGCLCPCSLGLGGVGMRLASTLVQVQQVRSVPVVPPKPQFAKMPSAMCSKIHVA
PANPCPRPGRLDGTPGERAWGSRASRSSWRNGGSLSFDAAVALARDRQRTEAQGVRRTQT
CTEGGDYCLIPRTSPCSMISAHSPRPLSCLELPSEGAEGSGSRSRLSLPPREPQVPDPLL
SSQRRSYAFETQANPGKGEGL
Target Bioclass
Other
Subcellular location
Cytoplasmic vesicle
Function GTPase-activating protein (GAP) for RAC1 and RHOA, but not for CDC42.
Uniprot ID
Q7Z6I6
Ensemble ID
ENST00000368013.8
HGNC ID
HGNC:27414

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
8505C SNV: p.P354T .
HCC15 SNV: p.S180F .
KATOIII SNV: p.P354T .
MDAMB453 SNV: p.D339N .
MOLT4 SNV: p.E242D .
NCIH358 SNV: p.P347A .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C954(2.54)  LDD3339  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [2]
Lodoacetamide azide
 Probe Info  		C965(0.00); C20(0.00); C40(0.00)  LDD0037  [2]
Compound 10
 Probe Info  		N.A.  LDD2216  [3]
Compound 11
 Probe Info  		N.A.  LDD2213  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C965(1.79)  LDD2187  [4]
 LDCM0572  Fragment10 Ramos C965(1.53)  LDD2189  [4]
 LDCM0573  Fragment11 Ramos C965(1.43)  LDD2190  [4]
 LDCM0574  Fragment12 Ramos C965(0.72)  LDD2191  [4]
 LDCM0575  Fragment13 Ramos C965(0.78)  LDD2192  [4]
 LDCM0576  Fragment14 Ramos C965(1.16)  LDD2193  [4]
 LDCM0579  Fragment20 Ramos C965(1.15)  LDD2194  [4]
 LDCM0580  Fragment21 Ramos C965(0.96)  LDD2195  [4]
 LDCM0582  Fragment23 Ramos C965(1.22)  LDD2196  [4]
 LDCM0578  Fragment27 Ramos C965(0.96)  LDD2197  [4]
 LDCM0586  Fragment28 Ramos C965(0.55)  LDD2198  [4]
 LDCM0588  Fragment30 Ramos C965(1.17)  LDD2199  [4]
 LDCM0589  Fragment31 Ramos C965(1.17)  LDD2200  [4]
 LDCM0590  Fragment32 Ramos C965(1.61)  LDD2201  [4]
 LDCM0468  Fragment33 Ramos C965(1.07)  LDD2202  [4]
 LDCM0596  Fragment38 Ramos C965(1.22)  LDD2203  [4]
 LDCM0566  Fragment4 Ramos C965(1.01)  LDD2184  [4]
 LDCM0610  Fragment52 Ramos C965(1.10)  LDD2204  [4]
 LDCM0614  Fragment56 Ramos C965(1.00)  LDD2205  [4]
 LDCM0569  Fragment7 Ramos C965(0.97)  LDD2186  [4]
 LDCM0571  Fragment9 Ramos C965(0.88)  LDD2188  [4]
 LDCM0022  KB02 T cell-activated C954(5.69); C1021, C1028(8.65)  LDD1704  [5]
 LDCM0023  KB03 Ramos C965(0.99)  LDD2183  [4]
 LDCM0024  KB05 NALM-6 C954(2.54)  LDD3339  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Rho-related GTP-binding protein RhoU (RHOU) Rho family Q7L0Q8

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
4 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
5 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.