Details of the Target

General Information of Target

Target ID LDTP08111
Target Name Sprouty-related, EVH1 domain-containing protein 2 (SPRED2)
Gene Name SPRED2
Gene ID 200734
Synonyms
Sprouty-related, EVH1 domain-containing protein 2; Spred-2
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MTEETHPDDDSYIVRVKAVVMTRDDSSGGWFPQEGGGISRVGVCKVMHPEGNGRSGFLIH
GERQKDKLVVLECYVRKDLVYTKANPTFHHWKVDNRKFGLTFQSPADARAFDRGVRKAIE
DLIEGSTTSSSTIHNEAELGDDDVFTTATDSSSNSSQKREQPTRTISSPTSCEHRRIYTL
GHLHDSYPTDHYHLDQPMPRPYRQVSFPDDDEEIVRINPREKIWMTGYEDYRHAPVRGKY
PDPSEDADSSYVRFAKGEVPKHDYNYPYVDSSDFGLGEDPKGRGGSVIKTQPSRGKSRRR
KEDGERSRCVYCRDMFNHEENRRGHCQDAPDSVRTCIRRVSCMWCADSMLYHCMSDPEGD
YTDPCSCDTSDEKFCLRWMALIALSFLAPCMCCYLPLRACYHCGVMCRCCGGKHKAAA
Target Bioclass
Other
Subcellular location
Cell membrane
Function
Negatively regulates Ras signaling pathways and downstream activation of MAP kinases. Recruits and translocates NF1 to the cell membrane, thereby enabling NF1-dependent hydrolysis of active GTP-bound Ras to inactive GDP-bound Ras. Inhibits fibroblast growth factor (FGF)-induced retinal lens fiber differentiation, probably by inhibiting FGF-mediated phosphorylation of ERK1/2. Inhibits TGFB-induced epithelial-to-mesenchymal transition in lens epithelial cells.
Uniprot ID
Q7Z698
Ensemble ID
ENST00000356388.9
HGNC ID
HGNC:17722

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.M343V .
Ishikawa (Heraklio) 02 ER SNV: p.R398W .
KYSE410 SNV: p.C400Ter .
MCC13 SNV: p.S26F
Substitution: p.R220_E221delinsQK		 .
MELJUSO SNV: p.S332F .
MFE319 SNV: p.K289M; p.Q291Ter .
SKNSH SNV: p.Y187S .
SNU1 Deletion: p.R220GfsTer6 .
TOV21G Deletion: p.C390VfsTer16 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C326(1.33)  LDD3427  [1]
IA-alkyne
 Probe Info  		C172(1.18); C326(1.24)  LDD2182  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C172(0.78)  LDD1507  [3]
 LDCM0276  AC17 HEK-293T C172(0.71)  LDD1515  [3]
 LDCM0281  AC21 HEK-293T C172(1.00)  LDD1520  [3]
 LDCM0285  AC25 HEK-293T C172(0.82)  LDD1524  [3]
 LDCM0289  AC29 HEK-293T C172(0.95)  LDD1528  [3]
 LDCM0294  AC33 HEK-293T C172(0.88)  LDD1533  [3]
 LDCM0298  AC37 HEK-293T C172(1.15)  LDD1537  [3]
 LDCM0303  AC41 HEK-293T C172(1.05)  LDD1542  [3]
 LDCM0307  AC45 HEK-293T C172(0.96)  LDD1546  [3]
 LDCM0311  AC49 HEK-293T C172(0.87)  LDD1550  [3]
 LDCM0312  AC5 HEK-293T C172(0.95)  LDD1551  [3]
 LDCM0316  AC53 HEK-293T C172(1.08)  LDD1555  [3]
 LDCM0320  AC57 HEK-293T C172(0.88)  LDD1559  [3]
 LDCM0325  AC61 HEK-293T C172(1.07)  LDD1564  [3]
 LDCM0248  AKOS034007472 HEK-293T C172(0.98)  LDD1511  [3]
 LDCM0356  AKOS034007680 HEK-293T C172(0.78)  LDD1570  [3]
 LDCM0371  CL102 HEK-293T C172(0.94)  LDD1575  [3]
 LDCM0375  CL106 HEK-293T C172(0.76)  LDD1579  [3]
 LDCM0380  CL110 HEK-293T C172(0.99)  LDD1584  [3]
 LDCM0384  CL114 HEK-293T C172(0.91)  LDD1588  [3]
 LDCM0388  CL118 HEK-293T C172(0.97)  LDD1592  [3]
 LDCM0393  CL122 HEK-293T C172(0.82)  LDD1597  [3]
 LDCM0397  CL126 HEK-293T C172(0.97)  LDD1601  [3]
 LDCM0401  CL14 HEK-293T C172(1.02)  LDD1605  [3]
 LDCM0404  CL17 HEK-293T C172(0.94)  LDD1608  [3]
 LDCM0407  CL2 HEK-293T C172(1.28)  LDD1611  [3]
 LDCM0409  CL21 HEK-293T C172(1.14)  LDD1613  [3]
 LDCM0414  CL26 HEK-293T C172(0.93)  LDD1618  [3]
 LDCM0417  CL29 HEK-293T C172(0.76)  LDD1621  [3]
 LDCM0422  CL33 HEK-293T C172(0.98)  LDD1626  [3]
 LDCM0431  CL41 HEK-293T C172(0.88)  LDD1635  [3]
 LDCM0435  CL45 HEK-293T C172(1.25)  LDD1639  [3]
 LDCM0440  CL5 HEK-293T C172(1.12)  LDD1644  [3]
 LDCM0441  CL50 HEK-293T C172(1.10)  LDD1645  [3]
 LDCM0444  CL53 HEK-293T C172(0.87)  LDD1647  [3]
 LDCM0448  CL57 HEK-293T C172(1.01)  LDD1651  [3]
 LDCM0454  CL62 HEK-293T C172(1.10)  LDD1657  [3]
 LDCM0457  CL65 HEK-293T C172(0.91)  LDD1660  [3]
 LDCM0461  CL69 HEK-293T C172(1.08)  LDD1664  [3]
 LDCM0467  CL74 HEK-293T C172(0.84)  LDD1670  [3]
 LDCM0470  CL77 HEK-293T C172(0.86)  LDD1673  [3]
 LDCM0475  CL81 HEK-293T C172(0.95)  LDD1678  [3]
 LDCM0480  CL86 HEK-293T C172(1.08)  LDD1683  [3]
 LDCM0483  CL89 HEK-293T C172(0.77)  LDD1686  [3]
 LDCM0484  CL9 HEK-293T C172(0.99)  LDD1687  [3]
 LDCM0488  CL93 HEK-293T C172(1.18)  LDD1691  [3]
 LDCM0493  CL98 HEK-293T C172(1.03)  LDD1696  [3]
 LDCM0625  F8 Ramos C172(1.29); C326(1.37)  LDD2187  [2]
 LDCM0572  Fragment10 Ramos C172(0.52); C326(1.04)  LDD2189  [2]
 LDCM0573  Fragment11 Ramos C172(3.40); C326(1.00)  LDD2190  [2]
 LDCM0574  Fragment12 Ramos C172(1.03); C326(2.09)  LDD2191  [2]
 LDCM0575  Fragment13 Ramos C172(0.57); C326(0.68)  LDD2192  [2]
 LDCM0576  Fragment14 Ramos C172(0.52)  LDD2193  [2]
 LDCM0579  Fragment20 Ramos C172(0.60); C326(1.43)  LDD2194  [2]
 LDCM0580  Fragment21 Ramos C172(0.96); C326(0.84)  LDD2195  [2]
 LDCM0582  Fragment23 Ramos C172(1.24); C326(0.65)  LDD2196  [2]
 LDCM0578  Fragment27 Ramos C172(0.65); C326(0.71)  LDD2197  [2]
 LDCM0586  Fragment28 Ramos C172(0.35); C326(0.70)  LDD2198  [2]
 LDCM0588  Fragment30 Ramos C172(1.23); C326(1.60)  LDD2199  [2]
 LDCM0589  Fragment31 Ramos C172(0.54); C326(0.92)  LDD2200  [2]
 LDCM0590  Fragment32 Ramos C326(0.81)  LDD2201  [2]
 LDCM0468  Fragment33 Ramos C172(1.00); C326(1.01)  LDD2202  [2]
 LDCM0596  Fragment38 Ramos C172(0.75); C326(1.84)  LDD2203  [2]
 LDCM0566  Fragment4 Ramos C172(1.09); C326(0.86)  LDD2184  [2]
 LDCM0427  Fragment51 HEK-293T C172(1.10)  LDD1631  [3]
 LDCM0610  Fragment52 Ramos C326(1.21)  LDD2204  [2]
 LDCM0614  Fragment56 Ramos C172(1.47); C326(1.01)  LDD2205  [2]
 LDCM0569  Fragment7 Ramos C172(0.84); C326(1.12)  LDD2186  [2]
 LDCM0571  Fragment9 Ramos C172(0.50); C326(0.87)  LDD2188  [2]
 LDCM0022  KB02 Ramos C172(1.18); C326(1.24)  LDD2182  [2]
 LDCM0023  KB03 Ramos C172(0.61); C326(1.45)  LDD2183  [2]
 LDCM0024  KB05 SK-MEL-28 C326(1.33)  LDD3427  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402