Details of the Target

General Information of Target

Target ID LDTP07568
Target Name Tubulin alpha-3E chain (TUBA3E)
Gene Name TUBA3E
Gene ID 112714
Synonyms
Tubulin alpha-3E chain; EC 3.6.5.-; Alpha-tubulin 3E) [Cleaved into: Detyrosinated tubulin alpha-3E chain]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGK
HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAASNYARGHYTIGKEIVDLVLD
RIRKLADLCTGLQGFLIFHSFGGGTGSGFASLLMERLSVDYSKKSKLEFAIYPAPQVSTA
VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITA
SLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPAN
QMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPP
TVVPGGDLAKVQRAVCMLSNTTAIAEAWARLVHKFDLMYAKWAFVHWYVGEGMEEGEFSE
AREDLAALEKDCEEVGVDSVEAEAEEGEAY
Target Bioclass
Enzyme
Family
Tubulin family
Subcellular location
Cytoplasm, cytoskeleton
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Uniprot ID
Q6PEY2
Ensemble ID
ENST00000312988.9
HGNC ID
HGNC:20765
ChEMBL ID
CHEMBL2095182

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
ONAyne
 Probe Info  		K96(2.78)  LDD0274  [1]
AZ-9
 Probe Info  		D46(1.01); D47(1.01); D345(1.11); E386(1.02)  LDD2208  [2]
IA-alkyne
 Probe Info  		C347(5.93)  LDD2157  [3]
AHL-Pu-1
 Probe Info  		C295(7.28)  LDD0170  [4]
HHS-475
 Probe Info  		Y399(0.65); Y262(0.67); Y272(0.77); Y282(0.82)  LDD0264  [5]
HHS-465
 Probe Info  		Y108(9.72); Y224(10.00); Y262(8.16); Y272(8.53)  LDD2237  [6]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [7]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [7]
Acrolein
 Probe Info  		C295(0.00); H283(0.00); C376(0.00); H406(0.00)  LDD0217  [8]
Crotonaldehyde
 Probe Info  		C347(0.00); H266(0.00); H88(0.00); C376(0.00)  LDD0219  [8]
Methacrolein
 Probe Info  		C295(0.00); C376(0.00); H283(0.00); C347(0.00)  LDD0218  [8]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STS-1
 Probe Info  		N.A.  LDD0137  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA DM93 C295(7.28)  LDD0170  [4]
 LDCM0108  Chloroacetamide HeLa C295(0.00); C376(0.00); H406(0.00); H266(0.00)  LDD0222  [8]
 LDCM0116  HHS-0101 DM93 Y399(0.65); Y262(0.67); Y272(0.77); Y282(0.82)  LDD0264  [5]
 LDCM0117  HHS-0201 DM93 Y399(0.53); Y262(0.60); Y272(0.63); Y357(0.81)  LDD0265  [5]
 LDCM0118  HHS-0301 DM93 Y262(0.52); Y399(0.61); Y272(0.64); Y357(0.78)  LDD0266  [5]
 LDCM0119  HHS-0401 DM93 Y272(0.39); Y262(0.44); Y399(0.49); Y357(0.82)  LDD0267  [5]
 LDCM0120  HHS-0701 DM93 Y272(0.47); Y262(0.52); Y399(0.55); Y357(0.77)  LDD0268  [5]
 LDCM0107  IAA HeLa H283(0.00); C295(0.00); C376(0.00); H266(0.00)  LDD0221  [8]
 LDCM0109  NEM HeLa H283(0.00); H406(0.00); H88(0.00); H266(0.00)  LDD0223  [8]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
N-myc-interactor (NMI) NMI family Q13287
Four and a half LIM domains protein 3 (FHL3) . Q13643

The Drug(s) Related To This Target

Investigative
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Cyt997 . DB05147

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
3 From chemoproteomic-detected amino acids to genomic coordinates: insights into precise multi-omic data integration. Mol Syst Biol. 2021 Feb;17(2):e9840. doi: 10.15252/msb.20209840.
Mass spectrometry data entry: PXD022151
4 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
5 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
6 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
7 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
8 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
9 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.