Details of the Target

General Information of Target

Target ID LDTP07480
Target Name Major facilitator superfamily domain-containing protein 12 (MFSD12)
Gene Name MFSD12
Gene ID 126321
Synonyms
C19orf28; Major facilitator superfamily domain-containing protein 12
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGPGPPAAGAAPSPRPLSLVARLSYAVGHFLNDLCASMWFTYLLLYLHSVRAYSSRGAGL
LLLLGQVADGLCTPLVGYEADRAASCCARYGPRKAWHLVGTVCVLLSFPFIFSPCLGCGA
ATPEWAALLYYGPFIVIFQFGWASTQISHLSLIPELVTNDHEKVELTALRYAFTVVANIT
VYGAAWLLLHLQGSSRVEPTQDISISDQLGGQDVPVFRNLSLLVVGVGAVFSLLFHLGTR
ERRRPHAEEPGEHTPLLAPATAQPLLLWKHWLREPAFYQVGILYMTTRLIVNLSQTYMAM
YLTYSLHLPKKFIATIPLVMYLSGFLSSFLMKPINKCIGRNMTYFSGLLVILAFAAWVAL
AEGLGVAVYAAAVLLGAGCATILVTSLAMTADLIGPHTNSGAFVYGSMSFLDKVANGLAV
MAIQSLHPCPSELCCRACVSFYHWAMVAVTGGVGVAAALCLCSLLLWPTRLRRWDRDARP
Target Bioclass
Transporter and channel
Family
Major facilitator superfamily
Subcellular location
Melanosome membrane
Function
Transporter that mediates the import of cysteine into melanosomes, thereby regulating skin pigmentation. In melanosomes, cysteine import is required both for normal levels of cystine, the oxidized dimer of cysteine, and provide cysteine for the production of the cysteinyldopas used in pheomelanin synthesis, thereby regulating skin pigmentation. Also catalyzes import of cysteine into lysosomes in non-pigmented cells, regulating lysosomal cystine and cysteine storage, which is essnetial for redox homeostasis.
Uniprot ID
Q6NUT3
Ensemble ID
ENST00000355415.7
HGNC ID
HGNC:28299

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO792 SNV: p.T254P .
EFO27 SNV: p.L348R .
HEC1B SNV: p.R170G .
IGR39 Deletion: p.S400AfsTer9 .
MCC26 Substitution: p.S18F .
SW756 Deletion: p.F354LfsTer36 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
BTD
 Probe Info  		C87(0.91)  LDD2101  [1]
IPM
 Probe Info  		C87(32.96)  LDD1701  [1]
DBIA
 Probe Info  		C87(0.84)  LDD1508  [2]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [3]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
BD-F
 Probe Info  		V214(0.00); S206(0.00)  LDD0024  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C87(0.84)  LDD1508  [2]
 LDCM0277  AC18 HEK-293T C87(1.01)  LDD1516  [2]
 LDCM0279  AC2 HEK-293T C87(1.20)  LDD1518  [2]
 LDCM0281  AC21 HEK-293T C87(0.80)  LDD1520  [2]
 LDCM0286  AC26 HEK-293T C87(0.83)  LDD1525  [2]
 LDCM0289  AC29 HEK-293T C87(0.91)  LDD1528  [2]
 LDCM0295  AC34 HEK-293T C87(1.01)  LDD1534  [2]
 LDCM0298  AC37 HEK-293T C87(0.81)  LDD1537  [2]
 LDCM0304  AC42 HEK-293T C87(0.82)  LDD1543  [2]
 LDCM0307  AC45 HEK-293T C87(0.90)  LDD1546  [2]
 LDCM0312  AC5 HEK-293T C87(0.86)  LDD1551  [2]
 LDCM0313  AC50 HEK-293T C87(0.66)  LDD1552  [2]
 LDCM0316  AC53 HEK-293T C87(0.90)  LDD1555  [2]
 LDCM0321  AC58 HEK-293T C87(0.99)  LDD1560  [2]
 LDCM0325  AC61 HEK-293T C87(1.00)  LDD1564  [2]
 LDCM0248  AKOS034007472 HEK-293T C87(1.17)  LDD1511  [2]
 LDCM0405  CL18 HEK-293T C87(1.14)  LDD1609  [2]
 LDCM0409  CL21 HEK-293T C87(1.01)  LDD1613  [2]
 LDCM0419  CL30 HEK-293T C87(0.87)  LDD1623  [2]
 LDCM0422  CL33 HEK-293T C87(1.52)  LDD1626  [2]
 LDCM0432  CL42 HEK-293T C87(0.76)  LDD1636  [2]
 LDCM0435  CL45 HEK-293T C87(1.19)  LDD1639  [2]
 LDCM0445  CL54 HEK-293T C87(2.00)  LDD1648  [2]
 LDCM0448  CL57 HEK-293T C87(1.36)  LDD1651  [2]
 LDCM0451  CL6 HEK-293T C87(1.18)  LDD1654  [2]
 LDCM0458  CL66 HEK-293T C87(0.92)  LDD1661  [2]
 LDCM0461  CL69 HEK-293T C87(1.13)  LDD1664  [2]
 LDCM0471  CL78 HEK-293T C87(1.19)  LDD1674  [2]
 LDCM0475  CL81 HEK-293T C87(1.16)  LDD1678  [2]
 LDCM0484  CL9 HEK-293T C87(1.24)  LDD1687  [2]
 LDCM0485  CL90 HEK-293T C87(2.25)  LDD1688  [2]
 LDCM0488  CL93 HEK-293T C87(1.37)  LDD1691  [2]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C87(28.53)  LDD1702  [1]
 LDCM0023  KB03 MDA-MB-231 C87(32.96)  LDD1701  [1]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C87(0.91)  LDD2101  [1]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C87(0.17)  LDD2109  [1]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C86(0.48)  LDD2125  [1]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C87(0.28)  LDD2136  [1]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C87(0.72)  LDD2148  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Transmembrane protein 14B (TMEM14B) TMEM14 family Q9NUH8
Immunoglobulin
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
B-cell antigen receptor complex-associated protein alpha chain (CD79A) . P11912
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Serine-rich single-pass membrane protein 1 (SSMEM1) . Q8WWF3

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
3 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
4 Evaluation of fully-functionalized diazirine tags for chemical proteomic applications. Chem Sci. 2021 May 7;12(22):7839-7847. doi: 10.1039/d1sc01360b.
Mass spectrometry data entry: PXD025652