Details of the Target

General Information of Target

Target ID LDTP07434
Target Name CapZ-interacting protein (RCSD1)
Gene Name RCSD1
Gene ID 92241
Synonyms
CAPZIP; CapZ-interacting protein; Protein kinase substrate CapZIP; RCSD domain-containing protein 1
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MEERPAETNANVDNSASPSVAQLAGRFREQAAAAKETPASKPTRRKPPCSLPLFPPKVDL
GQNGEEKSPPNASHPPKFKVKSSPLIEKLQANLTFDPAALLPGASPKSPGLKAMVSPFHS
PPSTPSSPGVRSRPSEAEEVPVSFDQPPEGSHLPCYNKVRTRGSIKRRPPSRRFRRSQSD
CGELGDFRAVESSQQNGAKEEDGDEVLPSKSKAPGSPLSSEGAAGEGVRTLGPAEKPPLR
RSPSRTEKQEEDRATEEAKNGEKARRSSEEVDGQHPAQEEVPESPQTSGPEAENRCGSPR
EEKPAGEEAEMEKATEVKGERVQNEEVGPEHDSQETKKLEEGAAVKETPHSPPGGVKGGD
VPKQEKGKEKQQEGAVLEPGCSPQTGPAQLETSSEVQSEPAVPKPEDDTPVQDTKM
Target Bioclass
Other
Function Stress-induced phosphorylation of CAPZIP may regulate the ability of F-actin-capping protein to remodel actin filament assembly.
Uniprot ID
Q6JBY9
Ensemble ID
ENST00000367854.8
HGNC ID
HGNC:28310

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AGS SNV: p.E330D .
CCK81 SNV: p.S135G .
CORL88 SNV: p.E308K .
HGC27 SNV: p.K366N .
KMRC1 SNV: p.T230S .
MCC13 SNV: p.E3K .
OVKATE SNV: p.S120R .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K88(7.02)  LDD2218  [1]
DBIA
 Probe Info  		C181(1.38)  LDD3333  [2]
N1
 Probe Info  		N.A.  LDD0245  [3]
4-Iodoacetamidophenylacetylene
 Probe Info  		C155(0.00); C181(0.00); C49(0.00)  LDD0038  [4]
IA-alkyne
 Probe Info  		C155(0.00); C181(0.00); C49(0.00)  LDD0036  [4]
IPIAA_L
 Probe Info  		C181(0.00); C155(0.00)  LDD0031  [5]
Lodoacetamide azide
 Probe Info  		C155(0.00); C181(0.00); C49(0.00)  LDD0037  [4]
Compound 10
 Probe Info  		C155(0.00); C181(0.00); C49(0.00)  LDD2216  [6]
Compound 11
 Probe Info  		C155(0.00); C181(0.00)  LDD2213  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C181(2.19); C155(2.73)  LDD2187  [7]
 LDCM0572  Fragment10 Ramos C181(2.44); C155(20.00)  LDD2189  [7]
 LDCM0573  Fragment11 Ramos C181(1.96); C155(0.06)  LDD2190  [7]
 LDCM0574  Fragment12 Ramos C181(0.67); C155(0.69)  LDD2191  [7]
 LDCM0575  Fragment13 Ramos C181(1.18); C155(1.26)  LDD2192  [7]
 LDCM0576  Fragment14 Ramos C181(0.67); C155(0.69)  LDD2193  [7]
 LDCM0579  Fragment20 Ramos C181(0.78); C155(0.51)  LDD2194  [7]
 LDCM0580  Fragment21 Ramos C181(1.12); C155(1.28)  LDD2195  [7]
 LDCM0582  Fragment23 Ramos C181(1.45); C155(1.35)  LDD2196  [7]
 LDCM0578  Fragment27 Ramos C181(1.45); C155(1.64)  LDD2197  [7]
 LDCM0586  Fragment28 Ramos C181(1.05); C155(0.73)  LDD2198  [7]
 LDCM0588  Fragment30 Ramos C181(1.34); C155(1.29)  LDD2199  [7]
 LDCM0589  Fragment31 Ramos C181(1.22); C155(1.12)  LDD2200  [7]
 LDCM0590  Fragment32 Ramos C181(1.03); C155(0.79)  LDD2201  [7]
 LDCM0468  Fragment33 Ramos C181(1.67); C155(6.14)  LDD2202  [7]
 LDCM0596  Fragment38 Ramos C181(0.93); C155(1.12)  LDD2203  [7]
 LDCM0566  Fragment4 Ramos C181(1.03); C155(0.91)  LDD2184  [7]
 LDCM0610  Fragment52 Ramos C181(1.36); C155(1.21)  LDD2204  [7]
 LDCM0614  Fragment56 Ramos C181(1.50); C155(1.35)  LDD2205  [7]
 LDCM0569  Fragment7 Ramos C181(1.92); C155(0.72)  LDD2186  [7]
 LDCM0571  Fragment9 Ramos C181(1.53); C155(1.00)  LDD2188  [7]
 LDCM0022  KB02 T cell C181(11.90)  LDD1707  [8]
 LDCM0023  KB03 Ramos C181(1.05); C155(0.74)  LDD2183  [7]
 LDCM0024  KB05 MOLM-13 C181(1.38)  LDD3333  [2]
 LDCM0131  RA190 MM1.R C181(1.73); C381(1.35); C155(1.11)  LDD0304  [9]

References

1 Global profiling of lysine reactivity and ligandability in the human proteome. Nat Chem. 2017 Dec;9(12):1181-1190. doi: 10.1038/nchem.2826. Epub 2017 Jul 31.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
4 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
5 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
6 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
7 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
8 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
9 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.