Details of the Target

General Information of Target

Target ID LDTP07363
Target Name Adenosine deaminase-like protein (ADAL)
Gene Name ADAL
Gene ID 161823
Synonyms
ADAL1; Adenosine deaminase-like protein; EC 3.5.4.-; Adenosine deaminase-like protein isoform 1; N6-mAMP deaminase; HsMAPDA; N6-methyl-AMP aminohydrolase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MIEAEEQQPCKTDFYSELPKVELHAHLNGSISSHTMKKLIAQKPDLKIHDQMTVIDKGKK
RTLEECFQMFQTIHQLTSSPEDILMVTKDVIKEFADDGVKYLELRSTPRRENATGMTKKT
YVESILEGIKQSKQENLDIDVRYLIAVDRRGGPLVAKETVKLAEEFFLSTEGTVLGLDLS
GDPTVGQAKDFLEPLLEAKKAGLKLALHLSEIPNQKKETQILLDLLPDRIGHGTFLNSGE
GGSLDLVDFVRQHRIPLELCLTSNVKSQTVPSYDQHHFGFWYSIAHPSVICTDDKGVFAT
HLSQEYQLAAETFNLTQSQVWDLSYESINYIFASDSTRSELRKKWNHLKPRVLHI
Target Bioclass
Enzyme
Family
Metallo-dependent hydrolases superfamily, Adenosine and AMP deaminases family
Function
Catalyzes the hydrolysis of the free cytosolic methylated adenosine nucleotide N(6)-methyl-AMP (N6-mAMP) to produce inositol monophosphate (IMP) and methylamine. Is required for the catabolism of cytosolic N6-mAMP, which is derived from the degradation of mRNA containing N6-methylated adenine (m6A). Catalyzes the removal of different alkyl groups not only from N6-substituted purine or 2-aminopurine nucleoside monophosphates but also from O6-substituted compounds in vitro.
Uniprot ID
Q6DHV7
Ensemble ID
ENST00000389651.8
HGNC ID
HGNC:31853
ChEMBL ID
CHEMBL1795150

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.L126F .
HEC1B SNV: p.V122E; p.P256S .
MCAS SNV: p.T184I .
MCC13 SNV: p.L177F .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K47(2.27)  LDD0277  [1]
DBIA
 Probe Info  		C66(1.12)  LDD1507  [2]
4-Iodoacetamidophenylacetylene
 Probe Info  		C260(0.00); C66(0.00)  LDD0038  [3]
IA-alkyne
 Probe Info  		N.A.  LDD2241  [3]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [3]
NAIA_5
 Probe Info  		N.A.  LDD2225  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C66(1.12)  LDD1507  [2]
 LDCM0215  AC10 HEK-293T C66(1.06)  LDD1508  [2]
 LDCM0259  AC14 HEK-293T C260(0.98)  LDD1512  [2]
 LDCM0276  AC17 HEK-293T C66(1.07)  LDD1515  [2]
 LDCM0277  AC18 HEK-293T C66(1.07)  LDD1516  [2]
 LDCM0279  AC2 HEK-293T C66(0.94)  LDD1518  [2]
 LDCM0282  AC22 HEK-293T C260(1.08)  LDD1521  [2]
 LDCM0285  AC25 HEK-293T C66(1.05)  LDD1524  [2]
 LDCM0286  AC26 HEK-293T C66(0.86)  LDD1525  [2]
 LDCM0291  AC30 HEK-293T C260(0.87)  LDD1530  [2]
 LDCM0294  AC33 HEK-293T C66(0.87)  LDD1533  [2]
 LDCM0295  AC34 HEK-293T C66(1.02)  LDD1534  [2]
 LDCM0299  AC38 HEK-293T C260(1.00)  LDD1538  [2]
 LDCM0303  AC41 HEK-293T C66(1.13)  LDD1542  [2]
 LDCM0304  AC42 HEK-293T C66(1.19)  LDD1543  [2]
 LDCM0308  AC46 HEK-293T C260(1.04)  LDD1547  [2]
 LDCM0311  AC49 HEK-293T C66(1.03)  LDD1550  [2]
 LDCM0313  AC50 HEK-293T C66(0.96)  LDD1552  [2]
 LDCM0317  AC54 HEK-293T C260(0.95)  LDD1556  [2]
 LDCM0320  AC57 HEK-293T C66(0.95)  LDD1559  [2]
 LDCM0321  AC58 HEK-293T C66(0.96)  LDD1560  [2]
 LDCM0323  AC6 HEK-293T C260(1.02)  LDD1562  [2]
 LDCM0326  AC62 HEK-293T C260(0.97)  LDD1565  [2]
 LDCM0356  AKOS034007680 HEK-293T C66(1.04)  LDD1570  [2]
 LDCM0632  CL-Sc Hep-G2 C10(0.78)  LDD2227  [4]
 LDCM0368  CL10 HEK-293T C260(1.05)  LDD1572  [2]
 LDCM0404  CL17 HEK-293T C66(0.89)  LDD1608  [2]
 LDCM0405  CL18 HEK-293T C66(0.99)  LDD1609  [2]
 LDCM0410  CL22 HEK-293T C260(0.88)  LDD1614  [2]
 LDCM0417  CL29 HEK-293T C66(1.35)  LDD1621  [2]
 LDCM0419  CL30 HEK-293T C66(0.99)  LDD1623  [2]
 LDCM0423  CL34 HEK-293T C260(0.90)  LDD1627  [2]
 LDCM0431  CL41 HEK-293T C66(1.11)  LDD1635  [2]
 LDCM0432  CL42 HEK-293T C66(0.92)  LDD1636  [2]
 LDCM0436  CL46 HEK-293T C260(0.89)  LDD1640  [2]
 LDCM0440  CL5 HEK-293T C66(0.94)  LDD1644  [2]
 LDCM0444  CL53 HEK-293T C66(1.10)  LDD1647  [2]
 LDCM0445  CL54 HEK-293T C66(0.89)  LDD1648  [2]
 LDCM0449  CL58 HEK-293T C260(0.92)  LDD1652  [2]
 LDCM0451  CL6 HEK-293T C66(0.97)  LDD1654  [2]
 LDCM0457  CL65 HEK-293T C66(1.10)  LDD1660  [2]
 LDCM0458  CL66 HEK-293T C66(1.01)  LDD1661  [2]
 LDCM0463  CL70 HEK-293T C260(0.91)  LDD1666  [2]
 LDCM0470  CL77 HEK-293T C66(1.02)  LDD1673  [2]
 LDCM0471  CL78 HEK-293T C66(0.99)  LDD1674  [2]
 LDCM0476  CL82 HEK-293T C260(0.99)  LDD1679  [2]
 LDCM0483  CL89 HEK-293T C66(1.08)  LDD1686  [2]
 LDCM0485  CL90 HEK-293T C66(0.98)  LDD1688  [2]
 LDCM0489  CL94 HEK-293T C260(0.93)  LDD1692  [2]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264