Details of the Target

General Information of Target

Target ID LDTP07183
Target Name SH3 and PX domain-containing protein 2A (SH3PXD2A)
Gene Name SH3PXD2A
Gene ID 9644
Synonyms
FISH; KIAA0418; SH3MD1; TKS5; SH3 and PX domain-containing protein 2A; Adapter protein TKS5; Five SH3 domain-containing protein; SH3 multiple domains protein 1; Tyrosine kinase substrate with five SH3 domains
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MLAYCVQDATVVDVEKRRNPSKHYVYIINVTWSDSTSQTIYRRYSKFFDLQMQLLDKFPI
EGGQKDPKQRIIPFLPGKILFRRSHIRDVAVKRLKPIDEYCRALVRLPPHISQCDEVFRF
FEARPEDVNPPKEDYGSSKRKSVWLSSWAESPKKDVTGADATAEPMILEQYVVVSNYKKQ
ENSELSLQAGEVVDVIEKNESGWWFVSTSEEQGWVPATYLEAQNGTRDDSDINTSKTGEV
SKRRKAHLRRLDRRWTLGGMVNRQHSREEKYVTVQPYTSQSKDEIGFEKGVTVEVIRKNL
EGWWYIRYLGKEGWAPASYLKKAKDDLPTRKKNLAGPVEIIGNIMEISNLLNKKASGDKE
TPPAEGEGHEAPIAKKEISLPILCNASNGSAVGVPDRTVSRLAQGSPAVARIAPQRAQIS
SPNLRTRPPPRRESSLGFQLPKPPEPPSVEVEYYTIAEFQSCISDGISFRGGQKAEVIDK
NSGGWWYVQIGEKEGWAPASYIDKRKKPNLSRRTSTLTRPKVPPPAPPSKPKEAEEGPTG
ASESQDSPRKLKYEEPEYDIPAFGFDSEPELSEEPVEDRASGERRPAQPHRPSPASSLQR
ARFKVGESSEDVALEEETIYENEGFRPYAEDTLSARGSSGDSDSPGSSSLSLTRKNSPKS
GSPKSSSLLKLKAEKNAQAEMGKNHSSASFSSSITINTTCCSSSSSSSSSLSKTSGDLKP
RSASDAGIRGTPKVRAKKDADANAGLTSCPRAKPSVRPKPFLNRAESQSQEKMDISTLRR
QLRPTGQLRGGLKGSKSEDSELPPQTASEAPSEGSRRSSSDLITLPATTPPCPTKKEWEG
PATSYMTCSAYQKVQDSEISFPAGVEVQVLEKQESGWWYVRFGELEGWAPSHYLVLDENE
QPDPSGKELDTVPAKGRQNEGKSDSLEKIERRVQALNTVNQSKKATPPIPSKPPGGFGKT
SGTPAVKMRNGVRQVAVRPQSVFVSPPPKDNNLSCALRRNESLTATDGLRGVRRNSSFST
ARSAAAEAKGRLAERAASQGSDSPLLPAQRNSIPVSPVRPKPIEKSQFIHNNLKDVYVSI
ADYEGDEETAGFQEGVSMEVLERNPNGWWYCQILDGVKPFKGWVPSNYLEKKN
Target Bioclass
Other
Family
SH3PXD2 family
Subcellular location
Cytoplasm
Function
Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of amyloid-beta peptide.
Uniprot ID
Q5TCZ1
Ensemble ID
ENST00000355946.7
HGNC ID
HGNC:23664

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 Deletion: p.S529AfsTer43 .
C4II SNV: p.S985Y .
COLO320 SNV: p.S639P .
HCC15 SNV: p.R932L DBIA    Probe Info 
IGROV1 SNV: p.D358N .
MEWO SNV: p.S434F .
MM1S SNV: p.D56V .
NCIH23 SNV: p.I949F .
SNGM Deletion: p.S529AfsTer43
SNV: p.P523S		 .
YSCCC SNV: p.R427T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C749(1.16)  LDD3312  [1]
HHS-475
 Probe Info  		Y319(0.73)  LDD0264  [2]
IA-alkyne
 Probe Info  		C749(5.20)  LDD1704  [3]
Acrolein
 Probe Info  		N.A.  LDD0224  [4]
NAIA_4
 Probe Info  		N.A.  LDD2226  [5]
IPM
 Probe Info  		C749(0.00); C384(0.00)  LDD0147  [6]
TFBX
 Probe Info  		N.A.  LDD0148  [6]
VSF
 Probe Info  		C995(0.00); C749(0.00)  LDD0007  [7]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [8]
W1
 Probe Info  		N.A.  LDD0236  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0281  AC21 HEK-293T C848(1.29)  LDD1520  [10]
 LDCM0289  AC29 HEK-293T C848(1.03)  LDD1528  [10]
 LDCM0298  AC37 HEK-293T C848(0.98)  LDD1537  [10]
 LDCM0307  AC45 HEK-293T C848(1.51)  LDD1546  [10]
 LDCM0312  AC5 HEK-293T C848(0.99)  LDD1551  [10]
 LDCM0316  AC53 HEK-293T C848(1.14)  LDD1555  [10]
 LDCM0325  AC61 HEK-293T C848(1.04)  LDD1564  [10]
 LDCM0248  AKOS034007472 HEK-293T C848(1.02)  LDD1511  [10]
 LDCM0369  CL100 HEK-293T C995(1.09)  LDD1573  [10]
 LDCM0371  CL102 HEK-293T C995(1.11)  LDD1575  [10]
 LDCM0373  CL104 HEK-293T C995(1.08)  LDD1577  [10]
 LDCM0375  CL106 HEK-293T C995(1.05)  LDD1579  [10]
 LDCM0377  CL108 HEK-293T C995(1.04)  LDD1581  [10]
 LDCM0380  CL110 HEK-293T C995(0.95)  LDD1584  [10]
 LDCM0382  CL112 HEK-293T C995(1.09)  LDD1586  [10]
 LDCM0384  CL114 HEK-293T C995(1.15)  LDD1588  [10]
 LDCM0386  CL116 HEK-293T C995(1.01)  LDD1590  [10]
 LDCM0388  CL118 HEK-293T C995(0.99)  LDD1592  [10]
 LDCM0391  CL120 HEK-293T C995(1.08)  LDD1595  [10]
 LDCM0393  CL122 HEK-293T C995(1.07)  LDD1597  [10]
 LDCM0395  CL124 HEK-293T C995(1.17)  LDD1599  [10]
 LDCM0397  CL126 HEK-293T C995(1.09)  LDD1601  [10]
 LDCM0399  CL128 HEK-293T C995(1.10)  LDD1603  [10]
 LDCM0401  CL14 HEK-293T C995(1.18)  LDD1605  [10]
 LDCM0403  CL16 HEK-293T C995(1.12)  LDD1607  [10]
 LDCM0407  CL2 HEK-293T C995(1.12)  LDD1611  [10]
 LDCM0409  CL21 HEK-293T C848(0.89)  LDD1613  [10]
 LDCM0414  CL26 HEK-293T C995(0.96)  LDD1618  [10]
 LDCM0416  CL28 HEK-293T C995(0.98)  LDD1620  [10]
 LDCM0422  CL33 HEK-293T C848(0.98)  LDD1626  [10]
 LDCM0429  CL4 HEK-293T C995(1.17)  LDD1633  [10]
 LDCM0430  CL40 HEK-293T C995(1.13)  LDD1634  [10]
 LDCM0435  CL45 HEK-293T C848(1.27)  LDD1639  [10]
 LDCM0441  CL50 HEK-293T C995(1.09)  LDD1645  [10]
 LDCM0443  CL52 HEK-293T C995(1.05)  LDD1646  [10]
 LDCM0448  CL57 HEK-293T C848(0.79)  LDD1651  [10]
 LDCM0454  CL62 HEK-293T C995(1.01)  LDD1657  [10]
 LDCM0456  CL64 HEK-293T C995(0.93)  LDD1659  [10]
 LDCM0461  CL69 HEK-293T C848(1.08)  LDD1664  [10]
 LDCM0467  CL74 HEK-293T C995(0.99)  LDD1670  [10]
 LDCM0469  CL76 HEK-293T C995(1.09)  LDD1672  [10]
 LDCM0475  CL81 HEK-293T C848(1.32)  LDD1678  [10]
 LDCM0480  CL86 HEK-293T C995(1.05)  LDD1683  [10]
 LDCM0482  CL88 HEK-293T C995(1.05)  LDD1685  [10]
 LDCM0484  CL9 HEK-293T C848(0.86)  LDD1687  [10]
 LDCM0488  CL93 HEK-293T C848(0.79)  LDD1691  [10]
 LDCM0493  CL98 HEK-293T C995(1.04)  LDD1696  [10]
 LDCM0427  Fragment51 HEK-293T C995(1.06)  LDD1631  [10]
 LDCM0116  HHS-0101 DM93 Y319(0.73)  LDD0264  [2]
 LDCM0117  HHS-0201 DM93 Y319(0.68)  LDD0265  [2]
 LDCM0118  HHS-0301 DM93 Y319(0.71)  LDD0266  [2]
 LDCM0119  HHS-0401 DM93 Y319(1.14)  LDD0267  [2]
 LDCM0120  HHS-0701 DM93 Y319(0.84)  LDD0268  [2]
 LDCM0022  KB02 HEK-293T C749(1.01)  LDD1492  [10]
 LDCM0023  KB03 HEK-293T C749(1.25)  LDD1497  [10]
 LDCM0024  KB05 HMCB C749(1.16)  LDD3312  [1]
 LDCM0109  NEM HeLa N.A.  LDD0224  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Disintegrin and metalloproteinase domain-containing protein 15 (ADAM15) . Q13444
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Son of sevenless homolog 1 (SOS1) . Q07889

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
3 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
6 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
7 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
8 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
9 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
10 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402