Details of the Target

General Information of Target

Target ID LDTP07060
Target Name Alpha-tubulin N-acetyltransferase 1 (ATAT1)
Gene Name ATAT1
Gene ID 79969
Synonyms
C6orf134; MEC17; Alpha-tubulin N-acetyltransferase 1; Alpha-TAT; Alpha-TAT1; TAT; EC 2.3.1.108; Acetyltransferase mec-17 homolog
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MEFPFDVDALFPERITVLDQHLRPPARRPGTTTPARVDLQQQIMTIIDELGKASAKAQNL
SAPITSASRMQSNRHVVYILKDSSARPAGKGAIIGFIKVGYKKLFVLDDREAHNEVEPLC
ILDFYIHESVQRHGHGRELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQV
NNFVIFEGFFAHQHRPPAPSLRATRHSRAAAVDPTPAAPARKLPPKRAEGDIKPYSSSDR
EFLKVAVEPPWPLNRAPRRATPPAHPPPRSSSLGNSPERGPLRPFVPEQELLRSLRLCPP
HPTARLLLAADPGGSPAQRRRTRGTPPGLVAQSCCYSRHGGVNSSSPNTGNQDSKQGEQE
TKNRSASEEQALSQDGSGEKPMHTAPPQAPAPPAQSWTVGGDILNARFIRNLQERRSTRP
W
Target Type
Literature-reported
Target Bioclass
Enzyme
Family
Acetyltransferase ATAT1 family
Subcellular location
Cytoplasm
Function
Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly. {|HAMAP-Rule:MF_03130}.
TTD ID
T92639
Uniprot ID
Q5SQI0
DrugMap ID
TTWUHQ1
Ensemble ID
ENST00000318999.11
HGNC ID
HGNC:21186

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info  		C298(1.36)  LDD2182  [1]
DBIA
 Probe Info  		C298(1.02)  LDD1492  [2]
IPM
 Probe Info  		N.A.  LDD2156  [3]
AOyne
 Probe Info  		13.60  LDD0443  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0226  AC11 HEK-293T C298(0.95)  LDD1509  [2]
 LDCM0278  AC19 HEK-293T C298(0.97)  LDD1517  [2]
 LDCM0287  AC27 HEK-293T C298(1.01)  LDD1526  [2]
 LDCM0290  AC3 HEK-293T C298(1.06)  LDD1529  [2]
 LDCM0296  AC35 HEK-293T C298(1.01)  LDD1535  [2]
 LDCM0305  AC43 HEK-293T C298(0.94)  LDD1544  [2]
 LDCM0314  AC51 HEK-293T C298(1.04)  LDD1553  [2]
 LDCM0322  AC59 HEK-293T C298(0.91)  LDD1561  [2]
 LDCM0367  CL1 HEK-293T C298(0.98)  LDD1571  [2]
 LDCM0370  CL101 HEK-293T C298(0.93)  LDD1574  [2]
 LDCM0371  CL102 HEK-293T C298(1.01)  LDD1575  [2]
 LDCM0374  CL105 HEK-293T C298(0.95)  LDD1578  [2]
 LDCM0375  CL106 HEK-293T C298(1.10)  LDD1579  [2]
 LDCM0378  CL109 HEK-293T C298(0.90)  LDD1582  [2]
 LDCM0380  CL110 HEK-293T C298(1.02)  LDD1584  [2]
 LDCM0383  CL113 HEK-293T C298(1.10)  LDD1587  [2]
 LDCM0384  CL114 HEK-293T C298(1.14)  LDD1588  [2]
 LDCM0387  CL117 HEK-293T C298(1.02)  LDD1591  [2]
 LDCM0388  CL118 HEK-293T C298(1.06)  LDD1592  [2]
 LDCM0392  CL121 HEK-293T C298(0.88)  LDD1596  [2]
 LDCM0393  CL122 HEK-293T C298(0.93)  LDD1597  [2]
 LDCM0396  CL125 HEK-293T C298(0.77)  LDD1600  [2]
 LDCM0397  CL126 HEK-293T C298(0.94)  LDD1601  [2]
 LDCM0400  CL13 HEK-293T C298(0.87)  LDD1604  [2]
 LDCM0401  CL14 HEK-293T C298(1.03)  LDD1605  [2]
 LDCM0406  CL19 HEK-293T C298(0.94)  LDD1610  [2]
 LDCM0407  CL2 HEK-293T C298(1.04)  LDD1611  [2]
 LDCM0413  CL25 HEK-293T C298(0.88)  LDD1617  [2]
 LDCM0414  CL26 HEK-293T C298(1.12)  LDD1618  [2]
 LDCM0420  CL31 HEK-293T C298(0.94)  LDD1624  [2]
 LDCM0426  CL37 HEK-293T C298(1.01)  LDD1630  [2]
 LDCM0433  CL43 HEK-293T C298(1.01)  LDD1637  [2]
 LDCM0439  CL49 HEK-293T C298(0.85)  LDD1643  [2]
 LDCM0441  CL50 HEK-293T C298(0.98)  LDD1645  [2]
 LDCM0446  CL55 HEK-293T C298(1.06)  LDD1649  [2]
 LDCM0453  CL61 HEK-293T C298(0.80)  LDD1656  [2]
 LDCM0454  CL62 HEK-293T C298(0.91)  LDD1657  [2]
 LDCM0459  CL67 HEK-293T C298(1.06)  LDD1662  [2]
 LDCM0462  CL7 HEK-293T C298(0.97)  LDD1665  [2]
 LDCM0466  CL73 HEK-293T C298(0.87)  LDD1669  [2]
 LDCM0467  CL74 HEK-293T C298(1.02)  LDD1670  [2]
 LDCM0472  CL79 HEK-293T C298(1.03)  LDD1675  [2]
 LDCM0479  CL85 HEK-293T C298(0.75)  LDD1682  [2]
 LDCM0480  CL86 HEK-293T C298(0.76)  LDD1683  [2]
 LDCM0486  CL91 HEK-293T C298(0.88)  LDD1689  [2]
 LDCM0492  CL97 HEK-293T C298(1.03)  LDD1695  [2]
 LDCM0493  CL98 HEK-293T C298(1.02)  LDD1696  [2]
 LDCM0625  F8 Ramos C298(1.42)  LDD2187  [1]
 LDCM0572  Fragment10 Ramos C298(1.70)  LDD2189  [1]
 LDCM0573  Fragment11 Ramos C298(1.90)  LDD2190  [1]
 LDCM0574  Fragment12 Ramos C298(1.29)  LDD2191  [1]
 LDCM0575  Fragment13 Ramos C298(1.00)  LDD2192  [1]
 LDCM0576  Fragment14 Ramos C298(0.79)  LDD2193  [1]
 LDCM0579  Fragment20 Ramos C298(1.39)  LDD2194  [1]
 LDCM0580  Fragment21 Ramos C298(0.84)  LDD2195  [1]
 LDCM0582  Fragment23 Ramos C298(1.14)  LDD2196  [1]
 LDCM0578  Fragment27 Ramos C298(0.85)  LDD2197  [1]
 LDCM0586  Fragment28 Ramos C298(0.70)  LDD2198  [1]
 LDCM0588  Fragment30 Ramos C298(1.09)  LDD2199  [1]
 LDCM0589  Fragment31 Ramos C298(0.81)  LDD2200  [1]
 LDCM0590  Fragment32 Ramos C298(0.88)  LDD2201  [1]
 LDCM0468  Fragment33 Ramos C298(0.99)  LDD2202  [1]
 LDCM0596  Fragment38 Ramos C298(0.84)  LDD2203  [1]
 LDCM0566  Fragment4 Ramos C298(1.05)  LDD2184  [1]
 LDCM0427  Fragment51 HEK-293T C298(0.94)  LDD1631  [2]
 LDCM0610  Fragment52 Ramos C298(0.98)  LDD2204  [1]
 LDCM0614  Fragment56 Ramos C298(1.41)  LDD2205  [1]
 LDCM0569  Fragment7 Ramos C298(1.05)  LDD2186  [1]
 LDCM0571  Fragment9 Ramos C298(1.22)  LDD2188  [1]
 LDCM0022  KB02 HEK-293T C298(1.02)  LDD1492  [2]
 LDCM0023  KB03 HEK-293T C298(1.02)  LDD1497  [2]
 LDCM0024  KB05 HEK-293T C298(0.96)  LDD1502  [2]

References

1 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
3 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
4 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.