Details of the Target

General Information of Target

Target ID LDTP07006
Target Name FYVE, RhoGEF and PH domain-containing protein 3 (FGD3)
Gene Name FGD3
Gene ID 89846
Synonyms
ZFYVE5; FYVE, RhoGEF and PH domain-containing protein 3; Zinc finger FYVE domain-containing protein 5
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MESGRGSSTPPGPIAALGMPDTGPGSSSLGKLQALPVGPRAHCGDPVSLAAAGDGSPDIG
PTGELSGSLKIPNRDSGIDSPSSSVAGENFPCEEGLEAGPSPTVLGAHAEMALDSQVPKV
TPQEEADSDVGEEPDSENTPQKADKDAGLAQHSGPQKLLHIAQELLHTEETYVKRLHLLD
QVFCTRLTDAGIPPEVIMGIFSNISSIHRFHGQFLLPELKTRITEEWDTNPRLGDILQKL
APFLKMYGEYVKNFDRAVGLVSTWTQRSPLFKDVVHSIQKQEVCGNLTLQHHMLEPVQRV
PRYELLLKDYLKRLPQDAPDRKDAERSLELISTAANHSNAAIRKVEKMHKLLEVYEQLGG
EEDIVNPANELIKEGQIQKLSAKNGTPQDRHLFLFNSMILYCVPKLRLMGQKFSVREKMD
ISGLQVQDIVKPNTAHTFIITGRKRSLELQTRTEEEKKEWIQIIQATIEKHKQNSETFKA
FGGAFSQDEDPSLSPDMPITSTSPVEPVVTTEGSSGAAGLEPRKLSSKTRRDKEKQSCKS
CGETFNSITKRRHHCKLCGAVICGKCSEFKAENSRQSRVCRDCFLTQPVAPESTEKTPTA
DPQPSLLCGPLRLSESGETWSEVWAAIPMSDPQVLHLQGGSQDGRLPRTIPLPSCKLSVP
DPEERLDSGHVWKLQWAKQSWYLSASSAELQQQWLETLSTAAHGDTAQDSPGALQLQVPM
GAAAP
Target Bioclass
Other
Subcellular location
Cytoplasm
Function
Promotes the formation of filopodia. May activate CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape.
Uniprot ID
Q5JSP0
Ensemble ID
ENST00000337352.10
HGNC ID
HGNC:16027

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
COLO678 SNV: p.G4V DBIA    Probe Info 
G415 SNV: p.H208Q DBIA    Probe Info 
HCT15 Deletion: p.R302GfsTer10 .
JHH7 SNV: p.V261L .
JURKAT SNV: p.A435T; p.Q715Ter .
MDAMB231 SNV: p.P81S .
MFE319 SNV: p.H291Y .
MOLT4 SNV: p.L424F; p.K533N; p.E696Ter; p.A702S IA-alkyne    Probe Info 
SKES1 SNV: p.G410C .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C655(52.44)  LDD0209  [1]
4-Iodoacetamidophenylacetylene
 Probe Info  		C184(0.00); C608(0.00); C541(0.00); C655(0.00)  LDD0038  [2]
IA-alkyne
 Probe Info  		C184(0.00); C541(0.00); C655(0.00)  LDD0036  [2]
Lodoacetamide azide
 Probe Info  		C583(0.00); C184(0.00); C541(0.00)  LDD0037  [2]
NAIA_5
 Probe Info  		N.A.  LDD2224  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0625  F8 Ramos C184(1.14); C43(1.19)  LDD2187  [4]
 LDCM0572  Fragment10 Ramos C184(1.06); C43(1.27); C655(0.97)  LDD2189  [4]
 LDCM0573  Fragment11 Ramos C184(4.42); C43(0.35); C655(0.24)  LDD2190  [4]
 LDCM0574  Fragment12 Ramos C184(4.36); C43(1.02); C655(0.78)  LDD2191  [4]
 LDCM0575  Fragment13 Ramos C184(0.92); C43(0.63)  LDD2192  [4]
 LDCM0576  Fragment14 Ramos C184(4.88); C43(1.12)  LDD2193  [4]
 LDCM0579  Fragment20 Ramos C184(3.41); C43(0.86); C655(0.84)  LDD2194  [4]
 LDCM0580  Fragment21 Ramos C184(0.86); C43(0.87); C655(0.69)  LDD2195  [4]
 LDCM0582  Fragment23 Ramos C184(1.36); C43(1.15); C655(0.46)  LDD2196  [4]
 LDCM0578  Fragment27 Ramos C184(0.76); C43(1.32); C655(0.89)  LDD2197  [4]
 LDCM0586  Fragment28 Ramos C184(1.86); C43(1.57); C655(0.97)  LDD2198  [4]
 LDCM0588  Fragment30 Ramos C184(0.84); C43(1.13); C655(0.93)  LDD2199  [4]
 LDCM0589  Fragment31 Ramos C184(1.75); C43(1.04); C655(0.74)  LDD2200  [4]
 LDCM0590  Fragment32 Ramos C184(2.42); C43(1.53)  LDD2201  [4]
 LDCM0468  Fragment33 Ramos C184(0.85); C43(1.17); C655(0.84)  LDD2202  [4]
 LDCM0596  Fragment38 Ramos C184(1.67); C43(0.93); C655(0.79)  LDD2203  [4]
 LDCM0566  Fragment4 Ramos C184(1.85); C43(1.27); C655(0.43)  LDD2184  [4]
 LDCM0610  Fragment52 Ramos C184(1.23); C43(1.73)  LDD2204  [4]
 LDCM0614  Fragment56 Ramos C184(0.83); C43(0.94); C655(1.07)  LDD2205  [4]
 LDCM0569  Fragment7 Ramos C184(1.97); C43(1.15); C655(0.58)  LDD2186  [4]
 LDCM0571  Fragment9 Ramos C184(2.01); C43(1.20)  LDD2188  [4]
 LDCM0022  KB02 Ramos C184(2.61); C43(1.35); C655(0.63)  LDD2182  [4]
 LDCM0023  KB03 Jurkat C655(52.44)  LDD0209  [1]
 LDCM0024  KB05 HMCB C655(2.15)  LDD3312  [5]
 LDCM0131  RA190 MM1.R C43(1.30)  LDD0304  [6]

References

1 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
2 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
6 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.