Details of the Target

General Information of Target

Target ID LDTP06949
Target Name Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase (ALG10)
Gene Name ALG10
Gene ID 84920
Synonyms
ALG10A; Dol-P-Glc:Glc(2)Man(9)GlcNAc(2)-PP-Dol alpha-1,2-glucosyltransferase; EC 2.4.1.256; Alpha-1,2-glucosyltransferase ALG10-A; Alpha-2-glucosyltransferase ALG10-A; Asparagine-linked glycosylation protein 10 homolog A
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAQLEGYYFSAALSCTFLVSCLLFSAFSRALREPYMDEIFHLPQAQRYCEGHFSLSQWDP
MITTLPGLYLVSIGVIKPAIWIFGWSEHVVCSIGMLRFVNLLFSVGNFYLLYLLFCKVQP
RNKAASSIQRVLSTLTLAVFPTLYFFNFLYYTEAGSMFFTLFAYLMCLYGNHKTSAFLGF
CGFMFRQTNIIWAVFCAGNVIAQKLTEAWKTELQKKEDRLPPIKGPFAEFRKILQFLLAY
SMSFKNLSMLLLLTWPYILLGFLFCAFVVVNGGIVIGDRSSHEACLHFPQLFYFFSFTLF
FSFPHLLSPSKIKTFLSLVWKRRILFFVVTLVSVFLVWKFTYAHKYLLADNRHYTFYVWK
RVFQRYETVKYLLVPAYIFAGWSIADSLKSKSIFWNLMFFICLFTVIVPQKLLEFRYFIL
PYVIYRLNIPLPPTSRLICELSCYAVVNFITFFIFLNKTFQWPNSQDIQRFMW
Target Bioclass
Enzyme
Family
ALG10 glucosyltransferase family
Subcellular location
Endoplasmic reticulum membrane
Function
Adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc(2)Man(9)GlcNAc(2)-PP-Dol.
Uniprot ID
Q5BKT4
Ensemble ID
ENST00000266483.7
HGNC ID
HGNC:23162

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C181(0.63)  LDD1095  [1]
IPM
 Probe Info  		N.A.  LDD2156  [2]
AOyne
 Probe Info  		11.90  LDD0443  [3]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
OEA-DA
 Probe Info  		6.33  LDD0046  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0216  AC100 PaTu 8988t C181(0.63)  LDD1095  [1]
 LDCM0217  AC101 PaTu 8988t C181(0.53)  LDD1096  [1]
 LDCM0218  AC102 PaTu 8988t C181(0.49)  LDD1097  [1]
 LDCM0219  AC103 PaTu 8988t C181(0.65)  LDD1098  [1]
 LDCM0220  AC104 PaTu 8988t C181(0.68)  LDD1099  [1]
 LDCM0221  AC105 PaTu 8988t C181(0.58)  LDD1100  [1]
 LDCM0222  AC106 PaTu 8988t C181(0.59)  LDD1101  [1]
 LDCM0223  AC107 PaTu 8988t C181(0.60)  LDD1102  [1]
 LDCM0224  AC108 PaTu 8988t C181(0.75)  LDD1103  [1]
 LDCM0225  AC109 PaTu 8988t C181(0.50)  LDD1104  [1]
 LDCM0227  AC110 PaTu 8988t C181(0.64)  LDD1106  [1]
 LDCM0228  AC111 PaTu 8988t C181(0.52)  LDD1107  [1]
 LDCM0229  AC112 PaTu 8988t C181(0.65)  LDD1108  [1]
 LDCM0230  AC113 PaTu 8988t C181(1.33)  LDD1109  [1]
 LDCM0231  AC114 PaTu 8988t C181(0.96)  LDD1110  [1]
 LDCM0232  AC115 PaTu 8988t C181(1.39)  LDD1111  [1]
 LDCM0233  AC116 PaTu 8988t C181(1.23)  LDD1112  [1]
 LDCM0234  AC117 PaTu 8988t C181(1.38)  LDD1113  [1]
 LDCM0235  AC118 PaTu 8988t C181(1.13)  LDD1114  [1]
 LDCM0236  AC119 PaTu 8988t C181(1.15)  LDD1115  [1]
 LDCM0238  AC120 PaTu 8988t C181(1.10)  LDD1117  [1]
 LDCM0239  AC121 PaTu 8988t C181(1.00)  LDD1118  [1]
 LDCM0240  AC122 PaTu 8988t C181(1.14)  LDD1119  [1]
 LDCM0241  AC123 PaTu 8988t C181(1.18)  LDD1120  [1]
 LDCM0242  AC124 PaTu 8988t C181(0.92)  LDD1121  [1]
 LDCM0243  AC125 PaTu 8988t C181(1.28)  LDD1122  [1]
 LDCM0244  AC126 PaTu 8988t C181(0.95)  LDD1123  [1]
 LDCM0245  AC127 PaTu 8988t C181(0.65)  LDD1124  [1]
 LDCM0365  AC98 PaTu 8988t C181(0.73)  LDD1244  [1]
 LDCM0366  AC99 PaTu 8988t C181(0.74)  LDD1245  [1]
 LDCM0403  CL16 PaTu 8988t C181(0.68)  LDD1282  [1]
 LDCM0404  CL17 PaTu 8988t C181(0.78)  LDD1283  [1]
 LDCM0405  CL18 PaTu 8988t C181(0.72)  LDD1284  [1]
 LDCM0406  CL19 PaTu 8988t C181(0.68)  LDD1285  [1]
 LDCM0408  CL20 PaTu 8988t C181(0.49)  LDD1287  [1]
 LDCM0409  CL21 PaTu 8988t C181(0.53)  LDD1288  [1]
 LDCM0410  CL22 PaTu 8988t C181(0.47)  LDD1289  [1]
 LDCM0411  CL23 PaTu 8988t C181(0.63)  LDD1290  [1]
 LDCM0412  CL24 PaTu 8988t C181(0.78)  LDD1291  [1]
 LDCM0413  CL25 PaTu 8988t C181(0.47)  LDD1292  [1]
 LDCM0414  CL26 PaTu 8988t C181(0.49)  LDD1293  [1]
 LDCM0415  CL27 PaTu 8988t C181(0.89)  LDD1294  [1]
 LDCM0416  CL28 PaTu 8988t C181(0.40)  LDD1295  [1]
 LDCM0417  CL29 PaTu 8988t C181(0.50)  LDD1296  [1]
 LDCM0419  CL30 PaTu 8988t C181(0.61)  LDD1298  [1]
 LDCM0453  CL61 PaTu 8988t C181(0.62)  LDD1332  [1]
 LDCM0454  CL62 PaTu 8988t C181(0.99)  LDD1333  [1]
 LDCM0455  CL63 PaTu 8988t C181(1.10)  LDD1334  [1]
 LDCM0456  CL64 PaTu 8988t C181(0.93)  LDD1335  [1]
 LDCM0457  CL65 PaTu 8988t C181(0.77)  LDD1336  [1]
 LDCM0458  CL66 PaTu 8988t C181(0.36)  LDD1337  [1]
 LDCM0459  CL67 PaTu 8988t C181(0.72)  LDD1338  [1]
 LDCM0460  CL68 PaTu 8988t C181(0.67)  LDD1339  [1]
 LDCM0461  CL69 PaTu 8988t C181(0.55)  LDD1340  [1]
 LDCM0463  CL70 PaTu 8988t C181(0.66)  LDD1342  [1]
 LDCM0464  CL71 PaTu 8988t C181(0.96)  LDD1343  [1]
 LDCM0465  CL72 PaTu 8988t C181(1.64)  LDD1344  [1]
 LDCM0466  CL73 PaTu 8988t C181(0.86)  LDD1345  [1]
 LDCM0467  CL74 PaTu 8988t C181(0.93)  LDD1346  [1]
 LDCM0468  Fragment33 PaTu 8988t C181(0.77)  LDD1347  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Immunoglobulin
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
B-cell antigen receptor complex-associated protein alpha chain (CD79A) . P11912
Other
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Endoplasmic reticulum-Golgi intermediate compartment protein 3 (ERGIC3) ERGIC family Q9Y282
Stress-associated endoplasmic reticulum protein 2 (SERP2) RAMP4 family Q8N6R1
Protein shisa-like-1 (SHISAL1) Shisa family Q3SXP7
Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 2 protein (HERPUD2) . Q9BSE4

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
3 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
4 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570