Details of the Target

General Information of Target

Target ID LDTP06624
Target Name Kynurenine--oxoglutarate transaminase 1 (KYAT1)
Gene Name KYAT1
Gene ID 883
Synonyms
CCBL1; Kynurenine--oxoglutarate transaminase 1; EC 2.6.1.7; Cysteine-S-conjugate beta-lyase; EC 4.4.1.13; Glutamine transaminase K; GTK; Glutamine--phenylpyruvate transaminase; EC 2.6.1.64; Kynurenine aminotransferase 1; Kynurenine aminotransferase I; KATI; Kynurenine--oxoglutarate transaminase I
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAKQLQARRLDGIDYNPWVEFVKLASEHDVVNLGQGFPDFPPPDFAVEAFQHAVSGDFML
NQYTKTFGYPPLTKILASFFGELLGQEIDPLRNVLVTVGGYGALFTAFQALVDEGDEVII
IEPFFDCYEPMTMMAGGRPVFVSLKPGPIQNGELGSSSNWQLDPMELAGKFTSRTKALVL
NTPNNPLGKVFSREELELVASLCQQHDVVCITDEVYQWMVYDGHQHISIASLPGMWERTL
TIGSAGKTFSATGWKVGWVLGPDHIMKHLRTVHQNSVFHCPTQSQAAVAESFEREQLLFR
QPSSYFVQFPQAMQRCRDHMIRSLQSVGLKPIIPQGSYFLITDISDFKRKMPDLPGAVDE
PYDRRFVKWMIKNKGLVAIPVSIFYSVPHQKHFDHYIRFCFVKDEATLQAMDEKLRKWKV
EL
Target Bioclass
Enzyme
Family
Class-I pyridoxal-phosphate-dependent aminotransferase family
Subcellular location
Cytoplasm, cytosol
Function
Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA), an intermediate in the tryptophan catabolic pathway which is also a broad spectrum antagonist of the three ionotropic excitatory amino acid receptors among others. Also metabolizes the cysteine conjugates of certain halogenated alkenes and alkanes to form reactive metabolites. Catalyzes the beta-elimination of S-conjugates and Se-conjugates of L-(seleno)cysteine, resulting in the cleavage of the C-S or C-Se bond.
Uniprot ID
Q16773
Ensemble ID
ENST00000302586.8
HGNC ID
HGNC:1564
ChEMBL ID
CHEMBL3962

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
KMS12BM SNV: p.A53V .
MEWO SNV: p.Q409Ter .
OVK18 Deletion: p.G336AfsTer35 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Acrolein
 Probe Info  		N.A.  LDD0223  [1]
DBIA
 Probe Info  		C280(1.00)  LDD1513  [2]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [3]
IA-alkyne
 Probe Info  		C400(0.00); C280(0.00)  LDD0036  [3]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [3]
NAIA_4
 Probe Info  		N.A.  LDD2226  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0270  AC15 HEK-293T C280(1.00)  LDD1513  [2]
 LDCM0283  AC23 HEK-293T C280(1.06)  LDD1522  [2]
 LDCM0292  AC31 HEK-293T C280(1.05)  LDD1531  [2]
 LDCM0300  AC39 HEK-293T C280(1.03)  LDD1539  [2]
 LDCM0309  AC47 HEK-293T C280(1.07)  LDD1548  [2]
 LDCM0318  AC55 HEK-293T C280(1.02)  LDD1557  [2]
 LDCM0327  AC63 HEK-293T C280(1.07)  LDD1566  [2]
 LDCM0334  AC7 HEK-293T C280(1.00)  LDD1568  [2]
 LDCM0379  CL11 HEK-293T C280(1.55)  LDD1583  [2]
 LDCM0411  CL23 HEK-293T C280(1.24)  LDD1615  [2]
 LDCM0424  CL35 HEK-293T C280(1.58)  LDD1628  [2]
 LDCM0437  CL47 HEK-293T C280(1.36)  LDD1641  [2]
 LDCM0450  CL59 HEK-293T C280(1.33)  LDD1653  [2]
 LDCM0464  CL71 HEK-293T C280(1.54)  LDD1667  [2]
 LDCM0477  CL83 HEK-293T C280(1.35)  LDD1680  [2]
 LDCM0490  CL95 HEK-293T C280(1.24)  LDD1693  [2]
 LDCM0109  NEM HeLa N.A.  LDD0223  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Pachytene checkpoint protein 2 homolog (TRIP13) AAA ATPase family Q15645
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein VAC14 homolog (VAC14) VAC14 family Q08AM6

The Drug(s) Related To This Target

Approved
Click To Hide/Show 2 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
L-glutamine Small molecular drug DB00130
Pyridoxal Phosphate Small molecular drug DB00114
Investigative
Click To Hide/Show 4 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
D-phenylalanine Small molecular drug DB02556
Pyridoxamine-5'-phosphate Small molecular drug DB02142
Indoleacetic Acid . DB07950
N(6)-(Pyridoxal Phosphate)-l-lysine . DB04083

References

1 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264