Details of the Target

General Information of Target

Target ID LDTP06591
Target Name Nuclear factor interleukin-3-regulated protein (NFIL3)
Gene Name NFIL3
Gene ID 4783
Synonyms
E4BP4; IL3BP1; Nuclear factor interleukin-3-regulated protein; E4 promoter-binding protein 4; Interleukin-3 promoter transcriptional activator; Interleukin-3-binding protein 1; Transcriptional activator NF-IL3A
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MQLRKMQTVKKEQASLDASSNVDKMMVLNSALTEVSEDSTTGEELLLSEGSVGKNKSSAC
RRKREFIPDEKKDAMYWEKRRKNNEAAKRSREKRRLNDLVLENKLIALGEENATLKAELL
SLKLKFGLISSTAYAQEIQKLSNSTAVYFQDYQTSKSNVSSFVDEHEPSMVSSSCISVIK
HSPQSSLSDVSEVSSVEHTQESSVQGSCRSPENKFQIIKQEPMELESYTREPRDDRGSYT
ASIYQNYMGNSFSGYSHSPPLLQVNRSSSNSPRTSETDDGVVGKSSDGEDEQQVPKGPIH
SPVELKHVHATVVKVPEVNSSALPHKLRIKAKAMQIKVEAFDNEFEATQKLSSPIDMTSK
RHFELEKHSAPSMVHSSLTPFSVQVTNIQDWSLKSEHWHQKELSGKTQNSFKTGVVEMKD
SGYKVSDPENLYLKQGIANLSAEVVSLKRLIATQPISASDSG
Target Bioclass
Transcription factor
Family
BZIP family, NFIL3 subfamily
Subcellular location
Nucleus
Function
Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts. Represses transcriptional activity of PER1. Represses transcriptional activity of PER2 via the B-site on the promoter. Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. Protects pro-B cells from programmed cell death. Represses the transcription of CYP2A5. Positively regulates the expression and activity of CES2 by antagonizing the repressive action of NR1D1 on CES2. Required for the development of natural killer cell precursors.
Uniprot ID
Q16649
Ensemble ID
ENST00000297689.4
HGNC ID
HGNC:7787

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C175(1.02)  LDD1573  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0369  CL100 HEK-293T C175(1.02)  LDD1573  [1]
 LDCM0373  CL104 HEK-293T C175(0.89)  LDD1577  [1]
 LDCM0377  CL108 HEK-293T C175(1.04)  LDD1581  [1]
 LDCM0382  CL112 HEK-293T C175(0.93)  LDD1586  [1]
 LDCM0386  CL116 HEK-293T C175(0.97)  LDD1590  [1]
 LDCM0391  CL120 HEK-293T C175(1.20)  LDD1595  [1]
 LDCM0395  CL124 HEK-293T C175(0.98)  LDD1599  [1]
 LDCM0399  CL128 HEK-293T C175(1.00)  LDD1603  [1]
 LDCM0403  CL16 HEK-293T C175(1.12)  LDD1607  [1]
 LDCM0416  CL28 HEK-293T C175(0.82)  LDD1620  [1]
 LDCM0429  CL4 HEK-293T C175(0.89)  LDD1633  [1]
 LDCM0430  CL40 HEK-293T C175(1.03)  LDD1634  [1]
 LDCM0443  CL52 HEK-293T C175(0.83)  LDD1646  [1]
 LDCM0456  CL64 HEK-293T C175(0.59)  LDD1659  [1]
 LDCM0469  CL76 HEK-293T C175(1.16)  LDD1672  [1]
 LDCM0482  CL88 HEK-293T C175(1.02)  LDD1685  [1]

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402