Details of the Target

General Information of Target

Target ID LDTP06442
Target Name Twist-related protein 1 (TWIST1)
Gene Name TWIST1
Gene ID 7291
Synonyms
BHLHA38; TWIST; Twist-related protein 1; Class A basic helix-loop-helix protein 38; bHLHa38; H-twist
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MMQDVSSSPVSPADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGGGV
GGGDEPGSPAQGKRGKKSAGCGGGGGAGGGGGSSSGGGSPQSYEELQTQRVMANVRERQR
TQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDELDSKMASCSYVAH
ERLSYAFSVWRMEGAWSMSASH
Target Type
Literature-reported
Target Bioclass
Transcription factor
Subcellular location
Nucleus
Function
Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of pro-inflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-BMAL1 heterodimer.
TTD ID
T95660
Uniprot ID
Q15672
DrugMap ID
TTX1MY7
Ensemble ID
ENST00000242261.6
HGNC ID
HGNC:12428

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C175(2.38)  LDD3433  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0369  CL100 HEK-293T C175(1.72)  LDD1573  [2]
 LDCM0373  CL104 HEK-293T C175(1.20)  LDD1577  [2]
 LDCM0377  CL108 HEK-293T C175(0.92)  LDD1581  [2]
 LDCM0382  CL112 HEK-293T C175(1.01)  LDD1586  [2]
 LDCM0386  CL116 HEK-293T C175(0.98)  LDD1590  [2]
 LDCM0391  CL120 HEK-293T C175(1.16)  LDD1595  [2]
 LDCM0395  CL124 HEK-293T C175(1.08)  LDD1599  [2]
 LDCM0399  CL128 HEK-293T C175(1.04)  LDD1603  [2]
 LDCM0403  CL16 HEK-293T C175(1.19)  LDD1607  [2]
 LDCM0416  CL28 HEK-293T C175(1.10)  LDD1620  [2]
 LDCM0429  CL4 HEK-293T C175(1.06)  LDD1633  [2]
 LDCM0430  CL40 HEK-293T C175(0.82)  LDD1634  [2]
 LDCM0443  CL52 HEK-293T C175(1.41)  LDD1646  [2]
 LDCM0456  CL64 HEK-293T C175(1.01)  LDD1659  [2]
 LDCM0469  CL76 HEK-293T C175(1.04)  LDD1672  [2]
 LDCM0482  CL88 HEK-293T C175(1.15)  LDD1685  [2]
 LDCM0022  KB02 AGS C175(1.56)  LDD2263  [1]
 LDCM0023  KB03 AGS C81(2.07)  LDD2680  [1]
 LDCM0024  KB05 SK-N-AS C175(2.38)  LDD3433  [1]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402