Details of the Target

General Information of Target

Target ID LDTP06243
Target Name Nucleolar and coiled-body phosphoprotein 1 (NOLC1)
Gene Name NOLC1
Gene ID 9221
Synonyms
KIAA0035; NS5ATP13; Nucleolar and coiled-body phosphoprotein 1; 140 kDa nucleolar phosphoprotein; Nopp140; Hepatitis C virus NS5A-transactivated protein 13; HCV NS5A-transactivated protein 13; Nucleolar 130 kDa protein; Nucleolar phosphoprotein p130
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MADAGIRRVVPSDLYPLVLGFLRDNQLSEVANKFAKATGATQQDANASSLLDIYSFWLKS
AKVPERKLQANGPVAKKAKKKASSSDSEDSSEEEEEVQGPPAKKAAVPAKRVGLPPGKAA
AKASESSSSEESSDDDDEEDQKKQPVQKGVKPQAKAAKAPPKKAKSSDSDSDSSSEDEPP
KNQKPKITPVTVKAQTKAPPKPARAAPKIANGKAASSSSSSSSSSSSDDSEEEKAAATPK
KTVPKKQVVAKAPVKAATTPTRKSSSSEDSSSDEEEEQKKPMKNKPGPYSSVPPPSAPPP
KKSLGTQPPKKAVEKQQPVESSEDSSDESDSSSEEEKKPPTKAVVSKATTKPPPAKKAAE
SSSDSSDSDSSEDDEAPSKPAGTTKNSSNKPAVTTKSPAVKPAAAPKQPVGGGQKLLTRK
ADSSSSEEESSSSEEEKTKKMVATTKPKATAKAALSLPAKQAPQGSRDSSSDSDSSSSEE
EEEKTSKSAVKKKPQKVAGGAAPSKPASAKKGKAESSNSSSSDDSSEEEEEKLKGKGSPR
PQAPKANGTSALTAQNGKAAKNSEEEEEEKKKAAVVVSKSGSLKKRKQNEAAKEAETPQA
KKIKLQTPNTFPKRKKGEKRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQ
VLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFDSE
Target Bioclass
Other
Family
NOLC1 family
Subcellular location
Nucleus, nucleolus
Function
Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification. Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification. Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities.
Uniprot ID
Q14978
Ensemble ID
ENST00000405356.5
HGNC ID
HGNC:15608

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
ICC4 SNV: p.G548D .
JURKAT SNV: p.M282I .
KMCH1 SNV: p.E632D .
MEWO Substitution: p.S669F .
NCIH1703 SNV: p.M441I .
OCILY3 SNV: p.G684E .
RKO Deletion: p.K280NfsTer3 .
SNU869 SNV: p.G113C .
SW948 SNV: p.T607I .
VMRCRCW SNV: p.K602N .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 12 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C-Sul
 Probe Info  		3.95  LDD0066  [1]
ONAyne
 Probe Info  		K246(0.00); K62(0.00); K647(0.00); K33(0.00)  LDD0273  [2]
HHS-475
 Probe Info  		Y289(14.84)  LDD0264  [3]
AMP probe
 Probe Info  		N.A.  LDD0200  [4]
ATP probe
 Probe Info  		K33(0.00); K579(0.00); K505(0.00); K510(0.00)  LDD0199  [4]
N1
 Probe Info  		N.A.  LDD0245  [5]
NHS
 Probe Info  		K510(0.00); K33(0.00); K505(0.00); K647(0.00)  LDD0010  [6]
SF
 Probe Info  		K285(0.00); K510(0.00); Y289(0.00)  LDD0028  [7]
STPyne
 Probe Info  		N.A.  LDD0009  [6]
Ox-W18
 Probe Info  		N.A.  LDD2175  [8]
1c-yne
 Probe Info  		K59(0.00); K561(0.00); K67(0.00)  LDD0228  [9]
HHS-465
 Probe Info  		Y289(0.00); K285(0.00)  LDD2240  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0116  HHS-0101 DM93 Y289(14.84)  LDD0264  [3]
 LDCM0120  HHS-0701 DM93 Y289(12.24)  LDD0268  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Beta-arrestin-1 (ARRB1) Arrestin family P49407
Beta-arrestin-2 (ARRB2) Arrestin family P32121

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Doxorubicin Small molecular drug DB00997

References

1 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
4 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
5 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
6 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
7 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
8 Oxidative cyclization reagents reveal tryptophan cation- interactions. Nature. 2024 Mar;627(8004):680-687. doi: 10.1038/s41586-024-07140-6. Epub 2024 Mar 6.
Mass spectrometry data entry: PXD001377 , PXD005252
9 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
10 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010