Details of the Target

General Information of Target

Target ID LDTP05833
Target Name Nuclear body protein SP140 (SP140)
Gene Name SP140
Gene ID 11262
Synonyms
LYSP100; Nuclear body protein SP140; Lymphoid-restricted homolog of Sp100; LYSp100; Nuclear autoantigen Sp-140; Speckled 140 kDa
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAQQGQQGQMASGDSNLNFRMVAEIQNVEGQNLQEQVCPEPIFRFFRENKVEIASAITRP
FPFLMGLRDRSFISEQMYEHFQEAFRNLVPVTRVMYCVLSELEKTFGWSHLEALFSRINL
MAYPDLNEIYRSFQNVCYEHSPLQMNNVNDLEDRPRLLPYGKQENSNACHEMDDIAVPQE
ALSSSPRCEPGFSSESCEQLALPKAGGGDAEDAPSLLPGGGVSCKLAIQIDEGESEEMPK
LLPYDTEVLESNGMIDAARTYSTAPGEKQGEEEGRNSPRKRNQDKEKYQESPEGRDKETF
DLKTPQVTNEGEPEKGLCLLPGEGEEGSDDCSEMCDGEEPQEASSSLARCGSVSCLSAET
FDLKTPQVTNEGEPEKELSLLPGEGEEGSDDCSEMCDGEERQEASSSLARRGSVSSELEN
HPMNEEGESEELASSLLYDNVPGAEQSAYENEKCSCVMCFSEEVPGSPEARTESDQACGT
MDTVDIANNSTLGKPKRKRRKKRGHGWSRMRMRRQENSQQNDNSKADGQVVSSEKKANVN
LKDLSKIRGRKRGKPGTRFTQSDRAAQKRVRSRASRKHKDETVDFKAPLLPVTCGGVKGI
LHKKKLQQGILVKCIQTEDGKWFTPTEFEIKGGHARSKNWRLSVRCGGWPLRWLMENGFL
PDPPRIRYRKKKRILKSQNNSSVDPCMRNLDECEVCRDGGELFCCDTCSRVFHEDCHIPP
VEAERTPWNCIFCRMKESPGSQQCCQESEVLERQMCPEEQLKCEFLLLKVYCCSESSFFA
KIPYYYYIREACQGLKEPMWLDKIKKRLNEHGYPQVEGFVQDMRLIFQNHRASYKYKDFG
QMGFRLEAEFEKNFKEVFAIQETNGNN
Target Bioclass
Transcription factor
Subcellular location
Nucleus
Function
Component of the nuclear body, also known as nuclear domain 10, PML oncogenic domain, and KR body. May be involved in the pathogenesis of acute promyelocytic leukemia and viral infection. May play a role in chromatin-mediated regulation of gene expression although it does not bind to histone H3 tails.
Uniprot ID
Q13342
Ensemble ID
ENST00000343805.10
HGNC ID
HGNC:17133
ChEMBL ID
CHEMBL3108643

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
BT549 Insertion: p.W649GfsTer8 .
CAMA1 SNV: p.R576K .
COLO320 Insertion: p.W649GfsTer9 .
COLO678 Insertion: p.G647_G648dup .
COLO679 SNV: p.G599E .
DU145 SNV: p.R411S .
HCT15 SNV: p.Q289K .
IM95 Deletion: p.K672RfsTer4 .
KYSE150 SNV: p.C756Ter .
MOLT4 SNV: p.E849D .
NB1 SNV: p.E101Ter .
NCIH1793 SNV: p.S277N .
NCIH1993 SNV: p.V148I .
NCIH2172 SNV: p.Y784H .
NCIH2286 SNV: p.C456Ter; p.V644L .
OE33 SNV: p.I782S .
RKO Deletion: p.K672RfsTer4 .
VMRCRCW SNV: p.E426A .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C224(6.04)  LDD3372  [1]
IA-alkyne
 Probe Info  		C97(7.33)  LDD0513  [2]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0020  ARS-1620 HCC44 C686(1.23)  LDD2171  [4]
 LDCM0198  Dimethyl Fumarate(DMF) T cell C97(7.33)  LDD0513  [2]
 LDCM0022  KB02 T cell C97(5.70)  LDD1703  [2]
 LDCM0023  KB03 HG-3 C224(2.58)  LDD2772  [1]
 LDCM0024  KB05 OCI-Ly3 C224(6.04)  LDD3372  [1]
 LDCM0021  THZ1 HCT 116 C686(1.23)  LDD2173  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (PIN1) . Q13526
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Nuclear factor erythroid 2-related factor 2 (NFE2L2) BZIP family Q16236

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.