Details of the Target

General Information of Target

Target ID LDTP05671
Target Name Nuclear factor 1 A-type (NFIA)
Gene Name NFIA
Gene ID 4774
Synonyms
KIAA1439; Nuclear factor 1 A-type; NF1-A; Nuclear factor 1/A; CCAAT-box-binding transcription factor; CTF; Nuclear factor I/A; NF-I/A; NFI-A; TGGCA-binding protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MYSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKEEERAVKDEL
LSEKPEVKQKWASRLLAKLRKDIRPEYREDFVLTVTGKKPPCCVLSNPDQKGKMRRIDCL
RQADKVWRLDLVMVILFKGIPLESTDGERLVKSPQCSNPGLCVQPHHIGVSVKELDLYLA
YFVHAADSSQSESPSQPSDADIKDQPENGHLGFQDSFVTSGVFSVTELVRVSQTPIAAGT
GPNFSLSDLESSSYYSMSPGAMRRSLPSTSSTSSTKRLKSVEDEMDSPGEEPFYTGQGRS
PGSGSQSSGWHEVEPGMPSPTTLKKSEKSGFSSPSPSQTSSLGTAFTQHHRPVITGPRAS
PHATPSTLHFPTSPIIQQPGPYFSHPAIRYHPQETLKEFVQLVCPDAGQQAGQVGFLNPN
GSSQGKVHNPFLPTPMLPPPPPPPMARPVPLPVPDTKPPTTSTEGGAASPTSPTYSTPST
SPANRFVSVGPRDPSFVNIPQQTQSWYLG
Target Bioclass
Transcription factor
Family
CTF/NF-I family
Subcellular location
Nucleus
Function
Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
Uniprot ID
Q12857
Ensemble ID
ENST00000371187.7
HGNC ID
HGNC:7784

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 Deletion: p.F223LfsTer6 .
CMK SNV: p.S220Ter .
HT115 SNV: p.D176N .
IPC298 SNV: p.L79S .
JURKAT SNV: p.C6Y .
MOLT4 SNV: p.R47C .
NCIH1666 Insertion: p.Y2dup .
OVISE SNV: p.P141L .
RKO SNV: p.R492W .
RPMI7951 SNV: p.S220L .
SNU1 Insertion: p.G302RfsTer11 .
SW620 SNV: p.P101S .
TE11 SNV: p.Y390C .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		12.29  LDD0403  [1]
AHL-Pu-1
 Probe Info  		C404(8.15)  LDD0168  [2]
DBIA
 Probe Info  		C404(1.04)  LDD1492  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [4]
IPM
 Probe Info  		C119(0.00); C404(0.00)  LDD0147  [5]
TFBX
 Probe Info  		C119(0.00); C404(0.00)  LDD0148  [5]
AOyne
 Probe Info  		13.60  LDD0443  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C404(8.15)  LDD0168  [2]
 LDCM0026  4SU-RNA+native RNA HEK-293T C404(6.86)  LDD0169  [2]
 LDCM0215  AC10 HEK-293T C103(0.97)  LDD1508  [3]
 LDCM0277  AC18 HEK-293T C103(1.12)  LDD1516  [3]
 LDCM0279  AC2 HEK-293T C103(1.05)  LDD1518  [3]
 LDCM0281  AC21 HEK-293T C404(1.14)  LDD1520  [3]
 LDCM0286  AC26 HEK-293T C103(0.94)  LDD1525  [3]
 LDCM0289  AC29 HEK-293T C404(1.26)  LDD1528  [3]
 LDCM0295  AC34 HEK-293T C103(1.02)  LDD1534  [3]
 LDCM0298  AC37 HEK-293T C404(1.27)  LDD1537  [3]
 LDCM0304  AC42 HEK-293T C103(0.93)  LDD1543  [3]
 LDCM0307  AC45 HEK-293T C404(1.23)  LDD1546  [3]
 LDCM0312  AC5 HEK-293T C404(1.16)  LDD1551  [3]
 LDCM0313  AC50 HEK-293T C103(0.98)  LDD1552  [3]
 LDCM0316  AC53 HEK-293T C404(1.14)  LDD1555  [3]
 LDCM0321  AC58 HEK-293T C103(1.09)  LDD1560  [3]
 LDCM0325  AC61 HEK-293T C404(1.21)  LDD1564  [3]
 LDCM0248  AKOS034007472 HEK-293T C404(1.20)  LDD1511  [3]
 LDCM0156  Aniline NCI-H1299 12.29  LDD0403  [1]
 LDCM0371  CL102 HEK-293T C404(1.05)  LDD1575  [3]
 LDCM0375  CL106 HEK-293T C404(1.16)  LDD1579  [3]
 LDCM0380  CL110 HEK-293T C404(1.07)  LDD1584  [3]
 LDCM0384  CL114 HEK-293T C404(1.16)  LDD1588  [3]
 LDCM0388  CL118 HEK-293T C404(1.15)  LDD1592  [3]
 LDCM0393  CL122 HEK-293T C404(1.24)  LDD1597  [3]
 LDCM0397  CL126 HEK-293T C404(1.08)  LDD1601  [3]
 LDCM0401  CL14 HEK-293T C404(0.63)  LDD1605  [3]
 LDCM0405  CL18 HEK-293T C103(1.05)  LDD1609  [3]
 LDCM0407  CL2 HEK-293T C404(1.26)  LDD1611  [3]
 LDCM0409  CL21 HEK-293T C404(1.35)  LDD1613  [3]
 LDCM0414  CL26 HEK-293T C404(1.06)  LDD1618  [3]
 LDCM0419  CL30 HEK-293T C103(1.02)  LDD1623  [3]
 LDCM0422  CL33 HEK-293T C404(1.51)  LDD1626  [3]
 LDCM0432  CL42 HEK-293T C103(0.97)  LDD1636  [3]
 LDCM0435  CL45 HEK-293T C404(1.45)  LDD1639  [3]
 LDCM0441  CL50 HEK-293T C404(1.17)  LDD1645  [3]
 LDCM0445  CL54 HEK-293T C103(1.05)  LDD1648  [3]
 LDCM0448  CL57 HEK-293T C404(0.93)  LDD1651  [3]
 LDCM0451  CL6 HEK-293T C103(1.06)  LDD1654  [3]
 LDCM0454  CL62 HEK-293T C404(1.16)  LDD1657  [3]
 LDCM0458  CL66 HEK-293T C103(1.04)  LDD1661  [3]
 LDCM0461  CL69 HEK-293T C404(1.10)  LDD1664  [3]
 LDCM0467  CL74 HEK-293T C404(1.08)  LDD1670  [3]
 LDCM0471  CL78 HEK-293T C103(1.04)  LDD1674  [3]
 LDCM0475  CL81 HEK-293T C404(1.15)  LDD1678  [3]
 LDCM0480  CL86 HEK-293T C404(1.30)  LDD1683  [3]
 LDCM0484  CL9 HEK-293T C404(0.99)  LDD1687  [3]
 LDCM0485  CL90 HEK-293T C103(0.94)  LDD1688  [3]
 LDCM0488  CL93 HEK-293T C404(1.43)  LDD1691  [3]
 LDCM0493  CL98 HEK-293T C404(1.20)  LDD1696  [3]
 LDCM0427  Fragment51 HEK-293T C404(1.33)  LDD1631  [3]
 LDCM0022  KB02 HEK-293T C404(1.04)  LDD1492  [3]
 LDCM0023  KB03 HEK-293T C404(1.11)  LDD1497  [3]
 LDCM0024  KB05 HEK-293T C404(1.10)  LDD1502  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1) ATG8 family Q9H0R8
Pleckstrin homology domain-containing family F member 2 (PLEKHF2) . Q9H8W4

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
6 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.