General Information of Target

Target ID LDTP04920
Target Name Protein mago nashi homolog (MAGOH)
Gene Name MAGOH
Gene ID 4116
Synonyms
MAGOHA; Protein mago nashi homolog
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MESDFYLRYYVGHKGKFGHEFLEFEFRPDGKLRYANNSNYKNDVMIRKEAYVHKSVMEEL
KRIIDDSEITKEDDALWPPPDRVGRQELEIVIGDEHISFTTSKIGSLIDVNQSKDPEGLR
VFYYLVQDLKCLVFSLIGLHFKIKPI
Target Bioclass
Transporter and channel
Family
Mago nashi family
Subcellular location
Nucleus
Function
Required for pre-mRNA splicing as component of the spliceosome. Plays a redundant role with MAGOHB as core component of the exon junction complex (EJC) and in the nonsense-mediated decay (NMD) pathway. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly.
Uniprot ID
P61326
Ensemble ID
ENST00000371466.4
HGNC ID
HGNC:6815

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
SW403 SNV: p.L107F .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
CY-1
 Probe Info 
100.00  LDD0243  [1]
IPM
 Probe Info 
N.A.  LDD0241  [2]
Probe 1
 Probe Info 
Y10(17.98); Y40(344.17)  LDD3495  [3]
DBIA
 Probe Info 
C131(9.35)  LDD0204  [4]
HHS-475
 Probe Info 
Y40(0.96); Y34(1.84)  LDD0264  [5]
HHS-465
 Probe Info 
Y51(4.44)  LDD2237  [6]
ATP probe
 Probe Info 
K16(0.00); K41(0.00); K31(0.00)  LDD0199  [7]
4-Iodoacetamidophenylacetylene
 Probe Info 
N.A.  LDD0038  [8]
IA-alkyne
 Probe Info 
N.A.  LDD0036  [8]
Lodoacetamide azide
 Probe Info 
N.A.  LDD0037  [8]
SF
 Probe Info 
Y40(0.00); Y10(0.00); K14(0.00)  LDD0028  [9]
1c-yne
 Probe Info 
N.A.  LDD0228  [10]
NAIA_5
 Probe Info 
N.A.  LDD2223  [11]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0102  BDHI 8 Jurkat C131(9.35)  LDD0204  [4]
 LDCM0116  HHS-0101 DM93 Y40(0.96); Y34(1.84)  LDD0264  [5]
 LDCM0117  HHS-0201 DM93 Y40(0.87); Y34(11.65)  LDD0265  [5]
 LDCM0118  HHS-0301 DM93 Y40(0.93); Y34(1.57)  LDD0266  [5]
 LDCM0119  HHS-0401 DM93 Y40(0.74); Y34(0.97)  LDD0267  [5]
 LDCM0120  HHS-0701 DM93 Y40(1.00); Y34(1.81)  LDD0268  [5]
 LDCM0627  NUDT7-COV-1 HEK-293T C131(2.47)  LDD2206  [12]
 LDCM0628  OTUB2-COV-1 HEK-293T C131(1.33)  LDD2207  [12]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Eukaryotic initiation factor 4A-III (EIF4A3) DEAD box helicase family P38919
Ubiquitin thioesterase ZRANB1 (ZRANB1) Peptidase C64 family Q9UGI0
Transporter and channel
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein CASC3 (CASC3) CASC3 family O15234
Importin-13 (IPO13) Importin beta family O94829
RNA-binding protein 8A (RBM8A) RBM8A family Q9Y5S9
Transcription factor
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger protein with KRAB and SCAN domains 8 (ZKSCAN8) Krueppel C2H2-type zinc-finger protein family Q15776
Transcriptional adapter 2-alpha (TADA2A) . O75478
Other
Click To Hide/Show 7 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 (DPH2) DPH1/DPH2 family Q9BQC3
Golgin subfamily A member 2 (GOLGA2) GOLGA2 family Q08379
Partner of Y14 and mago (PYM1) Pym family Q9BRP8
Regulator of nonsense transcripts 3B (UPF3B) RENT3 family Q9BZI7
Centrosomal protein of 70 kDa (CEP70) . Q8NHQ1
Developmental pluripotency-associated protein 4 (DPPA4) . Q7L190
Protein phosphatase 1 regulatory inhibitor subunit 16B (PPP1R16B) . Q96T49

References

1 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
2 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
3 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
4 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
5 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
6 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
7 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
8 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
9 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
10 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
11 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
12 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.